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Protein

NAD kinase 1

Gene

nadK1

Organism
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei68 – 681Proton acceptorUniRule annotation
Binding sitei73 – 731NADUniRule annotation
Binding sitei186 – 1861NADUniRule annotation
Binding sitei205 – 2051NADUniRule annotation
Binding sitei240 – 2401NAD; via carbonyl oxygenUniRule annotation
Binding sitei275 – 2751NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi68 – 692NADUniRule annotation
Nucleotide bindingi175 – 1762NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAVE227882:GJU1-1042-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinase 1UniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinase 1UniRule annotation
Gene namesi
Name:nadK1UniRule annotation
Ordered Locus Names:SAV_1035
OrganismiStreptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680)
Taxonomic identifieri227882 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces
ProteomesiUP000000428 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 357357NAD kinase 1PRO_0000229698Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi227882.SAV_1035.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227223.
KOiK00858.
OMAiVLDPGDW.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.

Sequencei

Sequence statusi: Complete.

Q82P98-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVTRVGLVM HGGRAQAGDA ARIVRGWCAE RGVHCADIDV WRDDTRHSAR
60 70 80 90 100
EEVAAAGNPD LIVTLGGDGT FLRGARLAAE NDALVLGVDL GRVGFLTEVP
110 120 130 140 150
AAAVCEALEA VQEDRITVES RMLLTLRASR RLQVPTGMEA LLRYGRGPLL
160 170 180 190 200
PPPRVRTDCA EGDDWGIALH VTALNDIVLE KLARDRQVSV GVYLAGRLLA
210 220 230 240 250
SYSADALLVA TPTGSTAYSF AAGGPVVSPR AEALIFTPVA PHMAFNRSVV
260 270 280 290 300
AAPDEPIALR VLDRSGPAAV SVDGQLRGVL DPGDWIGVYA APRRLKAVRL
310 320 330 340 350
GPMDFYGRLR ERMNLTDAPA AVADGQAAPL WPVTSAPPAD LAHLTLPPGP

GDTPSAS
Length:357
Mass (Da):37,612
Last modified:June 1, 2003 - v1
Checksum:i4A17FAC7731B5C4D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000030 Genomic DNA. Translation: BAC68745.1.
RefSeqiNP_822210.1. NC_003155.4.
WP_010982473.1. NZ_BAVY01000030.1.

Genome annotation databases

EnsemblBacteriaiBAC68745; BAC68745; SAV_1035.
KEGGisma:SAV_1035.
PATRICi23715381. VBIStrAve112782_1108.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000030 Genomic DNA. Translation: BAC68745.1.
RefSeqiNP_822210.1. NC_003155.4.
WP_010982473.1. NZ_BAVY01000030.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi227882.SAV_1035.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC68745; BAC68745; SAV_1035.
KEGGisma:SAV_1035.
PATRICi23715381. VBIStrAve112782_1108.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227223.
KOiK00858.
OMAiVLDPGDW.
OrthoDBiEOG6PZXDR.

Enzyme and pathway databases

BioCyciSAVE227882:GJU1-1042-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites."
    Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M.
    Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.
  2. "Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis."
    Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S.
    Nat. Biotechnol. 21:526-531(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.

Entry informationi

Entry nameiNADK1_STRAW
AccessioniPrimary (citable) accession number: Q82P98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: June 1, 2003
Last modified: April 1, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.