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Protein

Exo-beta-D-glucosaminidase

Gene

csxA

Organism
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes chitosan and chitooligosaccharides with retention of anomeric configuration. Has no beta-mannosidase activity.By similarity1 Publication

Catalytic activityi

Hydrolysis of chitosan or chitosan oligosaccharides to remove successive D-glucosamine residues from the non-reducing termini.1 Publication

pH dependencei

Optimum pH is 5.3.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei476Proton donorBy similarity1
Active sitei545NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

  • chitin catabolic process Source: UniProtKB-KW
  • polysaccharide catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Exo-beta-D-glucosaminidaseImported (EC:3.2.1.165)
Gene namesi
Name:csxAImported
Ordered Locus Names:SAV_1223
OrganismiStreptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
Taxonomic identifieri227882 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces
Proteomesi
  • UP000000428 Componenti: Chromosome

Subcellular locationi

  • Secretedextracellular space By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Sequence analysisAdd BLAST32
ChainiPRO_000039904733 – 904Exo-beta-D-glucosaminidaseSequence analysisAdd BLAST872

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi227882.SAV_1223.

Structurei

3D structure databases

ProteinModelPortaliQ82NR8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Sequence analysis

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3250. LUCA.
HOGENOMiHOG000200333.
KOiK15855.
OMAiPWLYREE.
OrthoDBiPOG091H06CS.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR028829. Exo-b-D-glucosamin.
IPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10066:SF66. PTHR10066:SF66. 1 hit.
PfamiPF00703. Glyco_hydro_2. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 3 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q82NR8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFHRPASVRR FVTTAVALGL LSTLSTGARA GARTHEPPPR PTTVSSTAGS
60 70 80 90 100
TTALTGYAIQ STAKVTDPAA AVSSPGYPAS GWYPAGARST VLAALLAGGK
110 120 130 140 150
YADPFYSTNQ QKIPKADFQV PWWYRSDFTV ADTSARTYLD FSGVISAADV
160 170 180 190 200
FVNGRQIARS ADVAGAYTRH ELDVTSLVRE GANTVAFRIQ PNNPNKNLTM
210 220 230 240 250
GWIDWLEPPP DQNMGIVRDV LVRRGGPVAL RDAHVITRLD VPSLATADLT
260 270 280 290 300
VKARARNDSD AAITATVSGS VGATSFRRSV ALAAHETKTV TFTPADTPGL
310 320 330 340 350
HLTSPRVWWP AGMGGQPLYA LDLSASVSET VSDTVHESFG IRDVKAPLNS
360 370 380 390 400
DGARQYSVNG RRLLIKGGGW SPDEFLRWDS TYVEDRLRYA LDLGLNTIRL
410 420 430 440 450
EGHIEPDEFF DLADRYGILT LPGWECCNKW EGNVNGSGSG DEWTAADYPV
460 470 480 490 500
AKASMAAEAA RLRDHPSVVS FLIGSDFAPD AKIEKTYLDA LKAADWPTPV
510 520 530 540 550
VAAASDKSSP VSGSSGMKMT GPYDWIPPNY WYAKREGGAT GFNSETSAGP
560 570 580 590 600
DIPTLDTLRR MMTPAELDTL WKNPGAKQYH RSPSSVFGTL KIYDAALAGR
610 620 630 640 650
YGAPTGLTDY VRKAQLAQYE NVRAQFEAYG RGATDASKPA TGVIYWMFNS
660 670 680 690 700
GWTSLHWQLL DRYLDQGGAY FGAKKANEPL HVQYSYDDRS VVVVNNRPAA
710 720 730 740 750
VSGLTARVTL FNTDGTQKYD KSATGLSVAG DGAHSTALTL PSSVSGLSTT
760 770 780 790 800
YLARLVLTDS AGKEVSRNVY WLSTRPDTLD WAHTDWYYTP TTSYADLKGL
810 820 830 840 850
GSMARVPVSA TASTTAGTDG ASTTTVTVRN TGSGRTPSLF TDVHLVDSKG
860 870 880 890 900
KPVLPVQWSD NEVSLWPGES ATLTVTYRTA DLHGSAPRVR VSGWNTAEQT

VPAA
Length:904
Mass (Da):97,183
Last modified:June 1, 2003 - v1
Checksum:iC68F8507993D4C18
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000030 Genomic DNA. Translation: BAC68933.1.
RefSeqiWP_010982661.1. NZ_JZJK01000078.1.

Genome annotation databases

EnsemblBacteriaiBAC68933; BAC68933; SAVERM_1223.
KEGGisma:SAV_1223.
PATRICi23715777. VBIStrAve112782_1306.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000030 Genomic DNA. Translation: BAC68933.1.
RefSeqiWP_010982661.1. NZ_JZJK01000078.1.

3D structure databases

ProteinModelPortaliQ82NR8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi227882.SAV_1223.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC68933; BAC68933; SAVERM_1223.
KEGGisma:SAV_1223.
PATRICi23715777. VBIStrAve112782_1306.

Phylogenomic databases

eggNOGiCOG3250. LUCA.
HOGENOMiHOG000200333.
KOiK15855.
OMAiPWLYREE.
OrthoDBiPOG091H06CS.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR028829. Exo-b-D-glucosamin.
IPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10066:SF66. PTHR10066:SF66. 1 hit.
PfamiPF00703. Glyco_hydro_2. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 3 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEBDG_STRAW
AccessioniPrimary (citable) accession number: Q82NR8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.