Q82NI9 (GLSA_STRAW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutaminase
EC=3.5.1.2

Gene names

Name:	glsA
Ordered Locus Names:	SAV_1314

Organism

Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680) [Complete proteome] [HAMAP]

Taxonomic identifier

227882 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces › Streptomyces avermitilis

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-glutamine + H2O = L-glutamate + NH3. HAMAP-Rule MF_00313
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the glutaminase family.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutamine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	glutaminase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	Glutaminase HAMAP-Rule MF_00313		PRO_0000110626
Sites
	Binding site	73	1	Substrate By similarity
	Binding site	123	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	174	1	Substrate By similarity
	Binding site	198	1	Substrate By similarity
	Binding site	249	1	Substrate By similarity
	Binding site	267	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q82NI9 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: F77B2789DBC2781D	FASTA	313	33,299
	10 20 30 40 50 60 MVIMSASWSS HAFQPVLERI ADEIERTPGR GRPADYIPAL AACDPRRFGM AVAELDGTVY 70 80 90 100 110 120 GVGDWRQPFS TQSITKVFTL ALDLAREGDA LWEHVGREPS GNPFNSLVQL EYESGIPRNP 130 140 150 160 170 180 FINAGALVVT DRLQTQTGDA AGSLLEFLRA ESGNPRLTFD QDVAASEAAH GDRNAALGHF 190 200 210 220 230 240 MASYGNIDNS VPVLLDQYFR QCSIEASCAD LALATGFLAR HGIRADGSRL LTQSQAKQVN 250 260 270 280 290 300 AVMLTCGTYD AAGDFAYRVG LPGKSGVGGG IIAVVPGRCT LCVWSPGLDE RGNSVAGVAA 310 LDRFTTLTGV SVF

References

[1]

"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites." Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.

[2]

"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis." Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. Nat. Biotechnol. 21:526-531(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000030 Genomic DNA. Translation: BAC69024.1.
RefSeq	NP_822489.1. NC_003155.4.
3D structure databases
ProteinModelPortal	Q82NI9.
ModBase	Search...
Protein-protein interaction databases
STRING	227882.SAV_1314.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC69024; BAC69024; SAV_1314.
GeneID	1210180.
KEGG	sma:SAV_1314.
PATRIC	23715967. VBIStrAve112782_1401.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2066.
HOGENOM	HOG000216890.
KO	K01425.
OMA	EPSGNPF.
ProtClustDB	PRK00971.
Enzyme and pathway databases
BioCyc	SAVE227882:GJU1-1321-MONOMER.
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
HAMAP	MF_00313. Glutaminase.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR015868. Glutaminase. [Graphical view]
PANTHER	PTHR12544. PTHR12544. 1 hit.
Pfam	PF04960. Glutaminase. 1 hit. [Graphical view]
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
TIGRFAMs	TIGR03814. Gln_ase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLSA_STRAW

Accession

Primary (citable) accession number: Q82NI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program