Reviewed, UniProtKB/Swiss-Prot Q82ML4 (DXS1_STRAW)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose-5-phosphate synthase 1
    EC=2.2.1.7
Alternative name(s):
    1-deoxyxylulose-5-phosphate synthase 1
      Short name=DXP synthase 1
      Short name=DXPS 1

Gene names

Name:	dxs1
Ordered Locus Names:	SAV_1646

Organism

Streptomyces avermitilis [Complete proteome] [HAMAP]

Taxonomic identifier

33903 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	632 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity.
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 632	632	1-deoxy-D-xylulose-5-phosphate synthase 1 HAMAP MF_00315		PRO_0000189154

Sequences

	Sequence		Length	Mass (Da)
	Q82ML4-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 6BC5DF4412947086	FASTA	632	67,786
	10 20 30 40 50 60 MTMLENIRGP RDLKALSEAE LGELAEEIRE FLVHAVARTG GHLGPNLGVV ELSVALHRAF 70 80 90 100 110 120 ESPVDRILWD TGHQSYVHKL LTGRQDFSKL RSKGGLSGYP SREESEHDVI ENSHASTVLG 130 140 150 160 170 180 WADGLAKARQ VQGEKGHVVA VIGDGALTGG MAWEALNNIA AAKDRPLIIV VNDNERSYSP 190 200 210 220 230 240 TIGGLANHLA TLRTTDSYEK VLAWGKDVLL RTPVVGNTLY ESLHGAKKGF KDAFAPQGMF 250 260 270 280 290 300 EDLGLKYVGP IDGHDMKAME SAFRRAKRFH GPVLIHCLTE KGRGYEPALA HDEDRFHTVG 310 320 330 340 350 360 VMDPLTCEPL APSNGPSWTS VFGDEIVRIG EEREDVVAIT AAMLHPVGLN KFAEKFPGRV 370 380 390 400 410 420 WDVGIAEQHA AVSAAGLATG GLHPVVAVYA TFLNRAFDQL LMDVALHRCG VTFVLDRAGV 430 440 450 460 470 480 TGVDGASHNG MWDMSILQVV PGLRIAAPRD ADQLRAQLRE AVAVDDAPTL LRFPKESVGP 490 500 510 520 530 540 AIPAVDHVGG MDVLHRFPES PQVLLVAVGV MAPVCLQAAE LLEARGIGCT VVDPRWVKPV 550 560 570 580 590 600 DPSLPGLAAE HRLVAVVEDN SRAAGVGAAV ALALSDAEVD VPVRRFGIPE QFLAHAKRAE 610 620 630 VLADIGLTPV EIAGRISAGL ATKEELSKEK QE

References

[1]

"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites." Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed: 11572948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

[2]

"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis." Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. Nat. Biotechnol. 21:526-531(2003) [PubMed: 12692562] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000030 Genomic DNA. Translation: BAC69357.1.
RefSeq	NP_822822.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1210243.
GenomeReviews	Gene locus SAV_1646 in contig BA000030_GR.
KEGG	sma:SAV_1646.
NMPDR	fig|227882.1.peg.1648.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q82ML4.
OMA	MQELPHP.
Enzyme and pathway databases
BioCyc	SAVE227882:SAV1646-MON.
BRENDA	2.2.1.7. 140873.
Family and domain databases
HAMAP	MF_00315. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00204. dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS1_STRAW

Accession

Primary (citable) accession number: Q82ML4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)