Q82M83 (AROE_STRAW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shikimate dehydrogenase
EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	SAV_1777

Organism

Streptomyces avermitilis [Complete proteome] [HAMAP]

Taxonomic identifier

33903 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	nucleotide binding Inferred from electronic annotation. Source: InterPro shikimate 3-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	Shikimate dehydrogenase HAMAP MF_00222		PRO_0000325173
Regions
	Nucleotide binding	134 – 138	5	NADP By similarity
Sites
	Active site	74	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	Q82M83 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 1FEFEAE9E69D803F	FASTA	291	30,129
	10 20 30 40 50 60 MTQDSYLVGL IGSGIGPSLS PALHEREAGR QGLRYHYRLI DIDRLGVGPE AVGGLVRAAR 70 80 90 100 110 120 DLGFDGLNIT HPCKQLVISH LDTLAPQAEA LGAVNTVVFE DGRAVGHNTD VTGFAASFAR 130 140 150 160 170 180 GLPDARLERV VQLGAGGAGA AVAHALLTLG AGHVTVVDAL PERAAALAVA LNRHFGDGRA 190 200 210 220 230 240 AAASPDTLPK LLTDADGIVH ATPTGMAAHP GIPLPAELLH PGLWVAEVVY RPLETELLRT 250 260 270 280 290 ARALGCATLD GGGMAVFQAV DAFRLFTGRE PDSMRMLADI AELAGTAAVR H

References

[1]

"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis." Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. Nat. Biotechnol. 21:526-531(2003) [PubMed: 12692562] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

[2]

"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites." Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed: 11572948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000030 Genomic DNA. Translation: BAC69488.1.
RefSeq	NP_822953.1. NC_003155.4.
3D structure databases
HSSP	HSSP built from PDB template 1NVT based on UniProtKB Q58484.
ProteinModelPortal	Q82M83.
SMR	Q82M83. Positions 4-278.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1210282.
GenomeReviews	Gene locus SAV_1777 in contig BA000030_GR.
KEGG	sma:SAV_1777.
NMPDR	fig|227882.1.peg.1779.
PATRIC	23716955. VBIStrAve112782_1892.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG553408.
OMA	HEHEGDA.
ProtClustDB	PRK12549.
Enzyme and pathway databases
BioCyc	SAVE227882:SAV1777-MONOMER.
Family and domain databases
HAMAP	MF_00222. Shikimate_DH_AroE. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR013708. Shikimate_DH-bd_N. IPR022893. Shikimate_quinate_DH. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00014.
Pfam	PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROE_STRAW

Accession

Primary (citable) accession number: Q82M83

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program