Q82LG4 (METE_STRAW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	SAV_2046

Organism

Streptomyces avermitilis [Complete proteome] [HAMAP]

Taxonomic identifier

33903 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	772 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 772	772	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_0000098668
Sites
	Metal binding	656	1	Zinc By similarity
	Metal binding	658	1	Zinc By similarity
	Metal binding	741	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q82LG4 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 14251874DD7723EC	FASTA	772	83,151
	10 20 30 40 50 60 MTAKSAAAAA RATVYGYPRQ GRGRELKKAI EGYWKGRLDA DALRTTATGL RRDTWQQLAE 70 80 90 100 110 120 AGIHEVPTGD FSYYDHVLDT SVMVGAIPAR HRAAVEADAL DGYFAMARGT QDVAPLEMTK 130 140 150 160 170 180 WFDTNYHYLV PELGPDTVFS TDSAKQVAEL GEALALGLTA RPVLVGPVTY LLLAKPAPGV 190 200 210 220 230 240 AADFDPLTLL DRLLPVYAEV LADLRAAGAE WVQLDEPALV QDRTPAELNA AERAYRVLGG 250 260 270 280 290 300 LTDRPKLLVA SYFDRLGDAL PVLAEAPVEG LALDFAEGAA ANLDALAAVG GLPGKRLVAG 310 320 330 340 350 360 VVDGRNIWVN DLEKSLALLG TLLGLADRVD VAASCSLLHV PLDAAAERDI APQVLRWLAF 370 380 390 400 410 420 ARQKTAEIVT LAKGLGHGTD AIAAQLAANR ADLVSRADSA LTRDPAVRSR TAATTDADAR 430 440 450 460 470 480 RSGPYPERAA AQRARLGLPL LPTTTIGSFP QTAELRTARA DLRAGRIDSA GYEERIRAEI 490 500 510 520 530 540 GEVISFQEKA GLDVLVHGEA ERNDMVQYFA ERLTGYLTTQ HGWVQSYGTR YVRPPVLAGD 550 560 570 580 590 600 ISRPEPMTVP WTTYAQSLTD RPVKGMLTGP VTMLAWSFVR DDQPLGETAR QVALALRDEV 610 620 630 640 650 660 GDLEAAGTSV IQVDEPALRE TLPLRAADRP AYLAWATEAF RLTTGGVRPD TQIHTHMCYA 670 680 690 700 710 720 EFGDIVQAID DLDADVISLE AARSHMQVAR ELAEHAYPRE AGPGVYDIHS PRVPGVDETA 730 740 750 760 770 ALLRKGLEAI PAERLWVNPD CGLKTRGWPE TRASLENLVA AARTVRAEHA GS

References

[1]

"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites." Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed: 11572948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

[2]

"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis." Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. Nat. Biotechnol. 21:526-531(2003) [PubMed: 12692562] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000030 Genomic DNA. Translation: BAC69757.1.
RefSeq	NP_823222.1. NC_003155.4.
3D structure databases
ProteinModelPortal	Q82LG4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1210341.
GenomeReviews	Gene locus SAV_2046 in contig BA000030_GR.
KEGG	sma:SAV_2046.
NMPDR	fig|227882.1.peg.2048.
PATRIC	23717543. VBIStrAve112782_2185.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287495.
OMA	RNIWRAN.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	SAVE227882:SAV2046-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_STRAW

Accession

Primary (citable) accession number: Q82LG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program