Reviewed, UniProtKB/Swiss-Prot Q82KW8 (DXS2_STRAW)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose-5-phosphate synthase 2
    EC=2.2.1.7
Alternative name(s):
    1-deoxyxylulose-5-phosphate synthase 2
      Short name=DXP synthase 2
      Short name=DXPS 2

Gene names

Name:	dxs2
Ordered Locus Names:	SAV_2244

Organism

Streptomyces avermitilis [Complete proteome] [HAMAP]

Taxonomic identifier

33903 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	642 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity.
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 642	642	1-deoxy-D-xylulose-5-phosphate synthase 2 HAMAP MF_00315		PRO_0000189155

Sequences

	Sequence		Length	Mass (Da)
	Q82KW8-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: ABB834405185B7A3	FASTA	642	68,726
	10 20 30 40 50 60 MPLLTRIRGP RDLDRLSLEQ LDQLAEEIRT FLVDAVSKTG GHLGPNLGVV ELTIALHRVF 70 80 90 100 110 120 DSPKDRVLFD TGHQSYVHKL LTGRQDFSKL KMKGGLSGYP SQAESEHDVI ENSHASTVLG 130 140 150 160 170 180 WADGLAKANQ VLERDDHVVA VIGDGALTGG MAWEALNNIA AAKDRPLVIV VNDNERSYAP 190 200 210 220 230 240 TIGGLANHLA TLRTTDGYER FLARGKDLLE RTPVVGRPLY ETLHGAKKGL KDFIAPQGMF 250 260 270 280 290 300 EDLGLKYVGP IDGHDIEALE SALARAKRFG GPVIVHCLTE KGRGYQPALQ DEADRFHAVG 310 320 330 340 350 360 KIHPDTGLPI ASSGADWTSV FGEEMVKLGQ ERKDIVAITA AMLQPVGLDK FAKVFPKRVY 370 380 390 400 410 420 DVGIAEQHAA VSAAGLATGG LHPVFAVYAT FLNRAFDQVL MDVALHKCGV TFVLDRAGVT 430 440 450 460 470 480 GTDGASHNGM WDMSILQVVP GLRLAAPRDA DQVRAQLREA VDVDDAPTVV RFSKGAVGPA 490 500 510 520 530 540 VPAVGRIGGM DVLREPGTDA PDVLLVSVGA LAPMCLEIAG LLDKQGISAT VVDPRWVKPV 550 560 570 580 590 600 DEAMAPLAER HRVVVTVEDN SRVGGVGSAI AQALRDAGVD VPLRDFGIPP RFLDHASRGE 610 620 630 640 VMAEIGLTAP DIARQVTGLV SKLDGRFERP TAEIDSVEPA RD

References

[1]

"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites." Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed: 11572948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

[2]

"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis." Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. Nat. Biotechnol. 21:526-531(2003) [PubMed: 12692562] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000030 Genomic DNA. Translation: BAC69955.1.
RefSeq	NP_823420.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1210381.
GenomeReviews	Gene locus SAV_2244 in contig BA000030_GR.
KEGG	sma:SAV_2244.
NMPDR	fig|227882.1.peg.2246.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q82KW8.
OMA	QRFPDRY.
Enzyme and pathway databases
BioCyc	SAVE227882:SAV2244-MON.
BRENDA	2.2.1.7. 140873.
Family and domain databases
HAMAP	MF_00315. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00204. dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS2_STRAW

Accession

Primary (citable) accession number: Q82KW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)