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Q82KE2

- NRDJ_STRAW

UniProt

Q82KE2 - NRDJ_STRAW

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Protein

Vitamin B12-dependent ribonucleotide reductase

Gene

nrdJ

Organism
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactori

5'-deoxyadenosylcobalamine (coenzyme B12).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei142 – 1421SubstrateBy similarity
Binding sitei187 – 1871Substrate; via amide nitrogenBy similarity
Active sitei363 – 3631Proton acceptorBy similarity
Active sitei365 – 3651Cysteine radical intermediateBy similarity
Active sitei367 – 3671Proton acceptorBy similarity

GO - Molecular functioni

  1. cobalamin binding Source: UniProtKB-KW
  2. cobalt ion binding Source: InterPro
  3. nucleotide binding Source: UniProtKB-KW
  4. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

Cobalamin, Cobalt, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAVE227882:GJU1-2473-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin B12-dependent ribonucleotide reductase (EC:1.17.4.1)
Alternative name(s):
Ribonucleoside-diphosphate reductase NrdJ
Gene namesi
Name:nrdJ
Ordered Locus Names:SAV_2461
OrganismiStreptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680)
Taxonomic identifieri227882 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces
ProteomesiUP000000428: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 964964Vitamin B12-dependent ribonucleotide reductasePRO_0000231661Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi159 ↔ 376Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi227882.SAV_2461.

Structurei

3D structure databases

ProteinModelPortaliQ82KE2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni158 – 1592Substrate bindingBy similarity
Regioni363 – 3675Substrate bindingBy similarity
Regioni553 – 5575Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0209.
HOGENOMiHOG000021771.
KOiK00525.
OMAiGASMRIV.
OrthoDBiEOG6H4K5F.

Family and domain databases

InterProiIPR013678. RNR_2_N.
IPR000788. RNR_lg_C.
IPR013344. RNR_NrdJ/NrdZ.
IPR024434. TSCPD_dom.
[Graphical view]
PfamiPF08471. Ribonuc_red_2_N. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF12637. TSCPD. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02504. NrdJ_Z. 1 hit.

Sequencei

Sequence statusi: Complete.

Q82KE2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTETASGPAR GSRAKGTKAK GLRIERIHTT PGVHPYDEVE WARRDVVMTN
60 70 80 90 100
WRDGSVNFEQ RGVEFPDFWS VNAVNIVTSK YFRGAVGTPQ REVSLRQLID
110 120 130 140 150
RIVKTYRKAG EDYKYFASPA DAEIFEHELA YALLHQIFSF NSPVWFNVGT
160 170 180 190 200
PQPQQVSACF ILAVDDSMES ILDWYKEEGM IFKGGSGAGL NLSRIRSSKE
210 220 230 240 250
LLSSGGNASG PVSFMRGADA SAGTIKSGGA TRRAAKMVIL DVDHPDIEDF
260 270 280 290 300
IETKVKEEEK IRALRDAGFD MDLGGDDITS VQYQNANNSV RVNDTFMKAV
310 320 330 340 350
EEGGKFGLTS RMTGEVIEEV DAKSLFRKMA EAAWACADPG IQYDDTINHW
360 370 380 390 400
HTCPESGRIN GSNPCSEYMH LDNTSCNLAS LNLMKFLKDD GKGRQSFEVE
410 420 430 440 450
RFAKVVELVI TAMDISICFA DFPTQKIGEN TRAFRQLGIG YANLGALLMA
460 470 480 490 500
TGHAYDSDGG RALAGAITSL MTGTSYKRSA ELAAVVGPYD GYARNAQPHQ
510 520 530 540 550
RVMKQHSDAN GVAVRVDDLD TPIWAAATEA WQDVLHLGEK NGFRNAQASV
560 570 580 590 600
IAPTGTIGLA MSCDTTGLEP DLALVKFKKL VGGGSMQIVN GTVPQALRRL
610 620 630 640 650
GYQEEQIEAI VAHIAENGNV IDAPGLKHEH YEVFDCAMGE RSISAMGHVR
660 670 680 690 700
MMAAIQPWIS GALSKTVNLP ETATVEDVEE VYFEAWKMGV KALAIYRDNC
710 720 730 740 750
KVGQPLSAKK KETEKAEVTA KTEATIREAV EKVVEYRPVR KRLPKGRPGI
760 770 780 790 800
TTSFTVGGAE GYMTANSYPD DGLGEVFLKM SKQGSTLAGM MDAFSIAVSV
810 820 830 840 850
GLQYGVPLET YVSKFTNMRF EPAGMTDDPD VRMAQSIVDY IFRRLALDFL
860 870 880 890 900
PFETRSALGI HSAEERQRHL ETGSYEPSDD VDMDVEGLAQ SAPRAQELKA
910 920 930 940 950
VATPKAEVAA AVPAPKQAHT SAELVEMQLG IQADAPLCFS CGTKMQRAGS
960
CYICEGCGST SGCS
Length:964
Mass (Da):105,012
Last modified:June 1, 2003 - v1
Checksum:i2C634475A49C6B42
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000030 Genomic DNA. Translation: BAC70172.1.
RefSeqiNP_823637.1. NC_003155.4.

Genome annotation databases

EnsemblBacteriaiBAC70172; BAC70172; SAV_2461.
GeneIDi1210434.
KEGGisma:SAV_2461.
PATRICi23718421. VBIStrAve112782_2628.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000030 Genomic DNA. Translation: BAC70172.1 .
RefSeqi NP_823637.1. NC_003155.4.

3D structure databases

ProteinModelPortali Q82KE2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 227882.SAV_2461.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC70172 ; BAC70172 ; SAV_2461 .
GeneIDi 1210434.
KEGGi sma:SAV_2461.
PATRICi 23718421. VBIStrAve112782_2628.

Phylogenomic databases

eggNOGi COG0209.
HOGENOMi HOG000021771.
KOi K00525.
OMAi GASMRIV.
OrthoDBi EOG6H4K5F.

Enzyme and pathway databases

BioCyci SAVE227882:GJU1-2473-MONOMER.

Family and domain databases

InterProi IPR013678. RNR_2_N.
IPR000788. RNR_lg_C.
IPR013344. RNR_NrdJ/NrdZ.
IPR024434. TSCPD_dom.
[Graphical view ]
Pfami PF08471. Ribonuc_red_2_N. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF12637. TSCPD. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
TIGRFAMsi TIGR02504. NrdJ_Z. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites."
    Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M.
    Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.
  2. "Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis."
    Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S.
    Nat. Biotechnol. 21:526-531(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.

Entry informationi

Entry nameiNRDJ_STRAW
AccessioniPrimary (citable) accession number: Q82KE2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: June 1, 2003
Last modified: October 1, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3