Q82KE2 (NRDJ_STRAW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vitamin B12-dependent ribonucleotide reductase
EC=1.17.4.1

Alternative name(s):
Ribonucleoside-diphosphate reductase NrdJ

Gene names

Name:	nrdJ
Ordered Locus Names:	SAV_2461

Organism

Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680) [Complete proteome] [HAMAP]

Taxonomic identifier

227882 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces › Streptomyces avermitilis

Protein attributes

Sequence length	964 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Cofactor	5'-deoxyadenosylcobalamine (coenzyme B12) By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase class-2 family.

Ontologies

Keywords
Biological process	DNA synthesis
Ligand	Cobalamin Cobalt Nucleotide-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	cobalamin binding Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 964	964	Vitamin B12-dependent ribonucleotide reductase		PRO_0000231661
Sites
	Active site	159	1	By similarity
	Active site	365	1	By similarity
	Active site	376	1	By similarity
	Active site	957	1	By similarity
	Active site	963	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q82KE2 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2C634475A49C6B42	FASTA	964	105,012
	10 20 30 40 50 60 MTETASGPAR GSRAKGTKAK GLRIERIHTT PGVHPYDEVE WARRDVVMTN WRDGSVNFEQ 70 80 90 100 110 120 RGVEFPDFWS VNAVNIVTSK YFRGAVGTPQ REVSLRQLID RIVKTYRKAG EDYKYFASPA 130 140 150 160 170 180 DAEIFEHELA YALLHQIFSF NSPVWFNVGT PQPQQVSACF ILAVDDSMES ILDWYKEEGM 190 200 210 220 230 240 IFKGGSGAGL NLSRIRSSKE LLSSGGNASG PVSFMRGADA SAGTIKSGGA TRRAAKMVIL 250 260 270 280 290 300 DVDHPDIEDF IETKVKEEEK IRALRDAGFD MDLGGDDITS VQYQNANNSV RVNDTFMKAV 310 320 330 340 350 360 EEGGKFGLTS RMTGEVIEEV DAKSLFRKMA EAAWACADPG IQYDDTINHW HTCPESGRIN 370 380 390 400 410 420 GSNPCSEYMH LDNTSCNLAS LNLMKFLKDD GKGRQSFEVE RFAKVVELVI TAMDISICFA 430 440 450 460 470 480 DFPTQKIGEN TRAFRQLGIG YANLGALLMA TGHAYDSDGG RALAGAITSL MTGTSYKRSA 490 500 510 520 530 540 ELAAVVGPYD GYARNAQPHQ RVMKQHSDAN GVAVRVDDLD TPIWAAATEA WQDVLHLGEK 550 560 570 580 590 600 NGFRNAQASV IAPTGTIGLA MSCDTTGLEP DLALVKFKKL VGGGSMQIVN GTVPQALRRL 610 620 630 640 650 660 GYQEEQIEAI VAHIAENGNV IDAPGLKHEH YEVFDCAMGE RSISAMGHVR MMAAIQPWIS 670 680 690 700 710 720 GALSKTVNLP ETATVEDVEE VYFEAWKMGV KALAIYRDNC KVGQPLSAKK KETEKAEVTA 730 740 750 760 770 780 KTEATIREAV EKVVEYRPVR KRLPKGRPGI TTSFTVGGAE GYMTANSYPD DGLGEVFLKM 790 800 810 820 830 840 SKQGSTLAGM MDAFSIAVSV GLQYGVPLET YVSKFTNMRF EPAGMTDDPD VRMAQSIVDY 850 860 870 880 890 900 IFRRLALDFL PFETRSALGI HSAEERQRHL ETGSYEPSDD VDMDVEGLAQ SAPRAQELKA 910 920 930 940 950 960 VATPKAEVAA AVPAPKQAHT SAELVEMQLG IQADAPLCFS CGTKMQRAGS CYICEGCGST SGCS

References

[1]

"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites." Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.

[2]

"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis." Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. Nat. Biotechnol. 21:526-531(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000030 Genomic DNA. Translation: BAC70172.1.
RefSeq	NP_823637.1. NC_003155.4.
3D structure databases
ProteinModelPortal	Q82KE2.
ModBase	Search...
Protein-protein interaction databases
STRING	227882.SAV_2461.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC70172; BAC70172; SAV_2461.
GeneID	1210434.
KEGG	sma:SAV_2461.
PATRIC	23718421. VBIStrAve112782_2628.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0209.
HOGENOM	HOG000021771.
KO	K00525.
OMA	GVEHGYF.
ProtClustDB	PRK06556.
Enzyme and pathway databases
BioCyc	SAVE227882:GJU1-2473-MONOMER.
Family and domain databases
InterPro	IPR013678. RNR_2_N. IPR000788. RNR_lg_C. IPR013344. RNR_NrdJ/NrdZ. IPR024434. TSCPD_dom. [Graphical view]
Pfam	PF08471. Ribonuc_red_2_N. 1 hit. PF02867. Ribonuc_red_lgC. 1 hit. PF12637. TSCPD. 1 hit. [Graphical view]
PRINTS	PR01183. RIBORDTASEM1.
TIGRFAMs	TIGR02504. NrdJ_Z. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NRDJ_STRAW

Accession

Primary (citable) accession number: Q82KE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program