Reviewed,
UniProtKB/Swiss-Prot Q82KE2 (NRDJ_STRAW)
Last modified
November 3, 2009.
Version 43.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Vitamin B12-dependent ribonucleotide reductase EC=1.17.4.1 Alternative name(s): Ribonucleoside-diphosphate reductase nrdJ | ||||
| Gene names |
| ||||
| Organism | Streptomyces avermitilis [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 33903 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces |
Protein attributes
| Sequence length | 964 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen By similarity. |
| Catalytic activity | 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin. |
| Cofactor | 5'-deoxyadenosylcobalamine (coenzyme B12) By similarity. |
| Sequence similarities | Belongs to the ribonucleoside diphosphate reductase class-2 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | DNA synthesis |
| Ligand | Cobalamin Cobalt Nucleotide-binding |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | DNA replication Inferred from electronic annotation. Source: UniProtKB-KW oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro |
| Molecular function | class II ribonucleotide reductase activity Inferred from electronic annotation. Source: InterPro cobalamin bindingInferred from electronic annotation. Source: UniProtKB-KW cobalt ion bindingInferred from electronic annotation. Source: UniProtKB-KW nucleotide bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 964 | 964 | Vitamin B12-dependent ribonucleotide reductase | PRO_0000231661 | |||||
Sites | |||||||||
| Active site | 159 | 1 | By similarity | ||||||
| Active site | 365 | 1 | By similarity | ||||||
| Active site | 376 | 1 | By similarity | ||||||
| Active site | 957 | 1 | By similarity | ||||||
| Active site | 963 | 1 | By similarity | ||||||
Sequences
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References
| [1] | "Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites." Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed: 11572948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165. |
| [2] | "Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis." Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. Nat. Biotechnol. 21:526-531(2003) [PubMed: 12692562] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165. |
Cross-references
Sequence databases | |
|---|---|
| BA000030 Genomic DNA. Translation: BAC70172.1. | |
| RefSeq | NP_823637.1. |
3D structure databases | |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 1210434. |
| GenomeReviews | Gene locus SAV_2461 in contig BA000030_GR. |
| KEGG | sma:SAV_2461. |
| NMPDR | fig|227882.1.peg.2463. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | Q82KE2. |
| OMA | ARNAEAH. |
Enzyme and pathway databases | |
| BioCyc | SAVE227882:SAV2461-MON. |
| BRENDA | 1.17.4.1. 140873. |
Family and domain databases | |
| InterPro | IPR013344. RDP_Rdtase_AdoCbl-dep. IPR000788. Ribncl_red_lg_C. IPR013678. Ribonuc_red_2_N. [Graphical view] |
| PANTHER | PTHR11573. Ribncl_red_lg_C. 1 hit. |
| Pfam | PF08471. Ribonuc_red_2_N. 1 hit. PF02867. Ribonuc_red_lgC. 1 hit. [Graphical view] |
| PRINTS | PR01183. RIBORDTASEM1. |
| TIGRFAMs | TIGR02504. NrdJ_Z. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | NRDJ_STRAW | ||||||||
| Accession | Primary (citable) accession number: Q82KE2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||

Clusters with


