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Q82KE2 (NRDJ_STRAW) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vitamin B12-dependent ribonucleotide reductase

EC=1.17.4.1
Alternative name(s):
Ribonucleoside-diphosphate reductase NrdJ
Gene names
Name:nrdJ
Ordered Locus Names:SAV_2461
OrganismStreptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680) [Complete proteome] [HAMAP]
Taxonomic identifier227882 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length964 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen By similarity.

Catalytic activity

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactor

5'-deoxyadenosylcobalamine (coenzyme B12) By similarity.

Sequence similarities

Belongs to the ribonucleoside diphosphate reductase class-2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 964964Vitamin B12-dependent ribonucleotide reductase
PRO_0000231661

Regions

Region158 – 1592Substrate binding By similarity
Region363 – 3675Substrate binding By similarity
Region553 – 5575Substrate binding By similarity

Sites

Active site3631Proton acceptor By similarity
Active site3651Cysteine radical intermediate By similarity
Active site3671Proton acceptor By similarity
Binding site1421Substrate By similarity
Binding site1871Substrate; via amide nitrogen By similarity

Amino acid modifications

Disulfide bond159 ↔ 376Redox-active By similarity

Sequences

Sequence LengthMass (Da)Tools
Q82KE2 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 2C634475A49C6B42

FASTA964105,012
        10         20         30         40         50         60 
MTETASGPAR GSRAKGTKAK GLRIERIHTT PGVHPYDEVE WARRDVVMTN WRDGSVNFEQ 

        70         80         90        100        110        120 
RGVEFPDFWS VNAVNIVTSK YFRGAVGTPQ REVSLRQLID RIVKTYRKAG EDYKYFASPA 

       130        140        150        160        170        180 
DAEIFEHELA YALLHQIFSF NSPVWFNVGT PQPQQVSACF ILAVDDSMES ILDWYKEEGM 

       190        200        210        220        230        240 
IFKGGSGAGL NLSRIRSSKE LLSSGGNASG PVSFMRGADA SAGTIKSGGA TRRAAKMVIL 

       250        260        270        280        290        300 
DVDHPDIEDF IETKVKEEEK IRALRDAGFD MDLGGDDITS VQYQNANNSV RVNDTFMKAV 

       310        320        330        340        350        360 
EEGGKFGLTS RMTGEVIEEV DAKSLFRKMA EAAWACADPG IQYDDTINHW HTCPESGRIN 

       370        380        390        400        410        420 
GSNPCSEYMH LDNTSCNLAS LNLMKFLKDD GKGRQSFEVE RFAKVVELVI TAMDISICFA 

       430        440        450        460        470        480 
DFPTQKIGEN TRAFRQLGIG YANLGALLMA TGHAYDSDGG RALAGAITSL MTGTSYKRSA 

       490        500        510        520        530        540 
ELAAVVGPYD GYARNAQPHQ RVMKQHSDAN GVAVRVDDLD TPIWAAATEA WQDVLHLGEK 

       550        560        570        580        590        600 
NGFRNAQASV IAPTGTIGLA MSCDTTGLEP DLALVKFKKL VGGGSMQIVN GTVPQALRRL 

       610        620        630        640        650        660 
GYQEEQIEAI VAHIAENGNV IDAPGLKHEH YEVFDCAMGE RSISAMGHVR MMAAIQPWIS 

       670        680        690        700        710        720 
GALSKTVNLP ETATVEDVEE VYFEAWKMGV KALAIYRDNC KVGQPLSAKK KETEKAEVTA 

       730        740        750        760        770        780 
KTEATIREAV EKVVEYRPVR KRLPKGRPGI TTSFTVGGAE GYMTANSYPD DGLGEVFLKM 

       790        800        810        820        830        840 
SKQGSTLAGM MDAFSIAVSV GLQYGVPLET YVSKFTNMRF EPAGMTDDPD VRMAQSIVDY 

       850        860        870        880        890        900 
IFRRLALDFL PFETRSALGI HSAEERQRHL ETGSYEPSDD VDMDVEGLAQ SAPRAQELKA 

       910        920        930        940        950        960 
VATPKAEVAA AVPAPKQAHT SAELVEMQLG IQADAPLCFS CGTKMQRAGS CYICEGCGST 


SGCS 

« Hide

References

[1]"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites."
Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M.
Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.
[2]"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis."
Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S.
Nat. Biotechnol. 21:526-531(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000030 Genomic DNA. Translation: BAC70172.1.
RefSeqNP_823637.1. NC_003155.4.

3D structure databases

ProteinModelPortalQ82KE2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING227882.SAV_2461.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC70172; BAC70172; SAV_2461.
GeneID1210434.
KEGGsma:SAV_2461.
PATRIC23718421. VBIStrAve112782_2628.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0209.
HOGENOMHOG000021771.
KOK00525.
OMAGASMRIV.
OrthoDBEOG6H4K5F.

Enzyme and pathway databases

BioCycSAVE227882:GJU1-2473-MONOMER.

Family and domain databases

InterProIPR013678. RNR_2_N.
IPR000788. RNR_lg_C.
IPR013344. RNR_NrdJ/NrdZ.
IPR024434. TSCPD_dom.
[Graphical view]
PfamPF08471. Ribonuc_red_2_N. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF12637. TSCPD. 1 hit.
[Graphical view]
PRINTSPR01183. RIBORDTASEM1.
TIGRFAMsTIGR02504. NrdJ_Z. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNRDJ_STRAW
AccessionPrimary (citable) accession number: Q82KE2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: June 1, 2003
Last modified: May 14, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families