Reviewed, UniProtKB/Swiss-Prot Q82JV8 (SYP2_STRAW)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase 2
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase 2
      Short name=ProRS 2

Gene names

Name:	proS2
Ordered Locus Names:	SAV_2646

Organism

Streptomyces avermitilis [Complete proteome] [HAMAP]

Taxonomic identifier

33903 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity.
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	Prolyl-tRNA synthetase 2 HAMAP MF_01571		PRO_0000249151

Sequences

	Sequence		Length	Mass (Da)
	Q82JV8-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 5061F2E9DB52E331	FASTA	471	52,164
	10 20 30 40 50 60 MAKAPVLTPR ADDFPRWYQD LINKAELADN GPVRGTMVIR PYGYGLWERM QQEMDARIKE 70 80 90 100 110 120 TGTQNAYFPL LIPQSYLTKE ADHVEGFAPE LAVVTHGGGK ELEEPAVVRP TSEMIINDYF 130 140 150 160 170 180 SKWVQSYRDL PLLINQWANV VRWELRPRLF LRTTEFLWQE GHTAHATYEE ARDFAAHIHR 190 200 210 220 230 240 HVYADFMENV LAMDVVLGRK TAKERFAGAV NTLTLEGMMG DGKALQMGTS HELGQNFARA 250 260 270 280 290 300 FHTQYLSKEG KQELVWQTSW GSTTRMIGAL VMMHGDDNGL RVPPRLAQTQ VVVLAIKGDE 310 320 330 340 350 360 AVLAKVRETG DRLKAAGLRV QVDDRTDVPF GRRAVDWELK GVPVRVEVGP RDLENGTAMV 370 380 390 400 410 420 ARRIPGGKEP VALDALAALL PTALEEDQAL LLRQARERRA SRTSDVSTIE EAVEAAAGGG 430 440 450 460 470 WARIPWATLG ERGEAELAEH AASVRCLVAE DGSVPGADDA PGNVAVVARA Y

References

[1]

"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites." Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed: 11572948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

[2]

"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis." Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. Nat. Biotechnol. 21:526-531(2003) [PubMed: 12692562] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000030 Genomic DNA. Translation: BAC70357.1.
RefSeq	NP_823822.1.
3D structure databases
HSSP	HSSP built from PDB template 1HC7 based on UniProtKB Q93N97.
ModBase	Search...
Genome annotation databases
GeneID	1210491.
GenomeReviews	Gene locus SAV_2646 in contig BA000030_GR.
KEGG	sma:SAV_2646.
NMPDR	fig|227882.1.peg.2648.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q82JV8.
OMA	CIEAMMQ.
Enzyme and pathway databases
BioCyc	SAVE227882:SAV2646-MON.
BRENDA	6.1.1.15. 140873.
Family and domain databases
HAMAP	MF_01571. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR004499. Pro-tRNA-synth_IIa_pro-type. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00408. proS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP2_STRAW

Accession

Primary (citable) accession number: Q82JV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)