Q82JI2 (GCST_STRAW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminomethyltransferase
EC=2.1.2.10

Alternative name(s):
Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	SAV_2773

Organism

Streptomyces avermitilis [Complete proteome] [HAMAP]

Taxonomic identifier

33903 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity. HAMAP MF_00259
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the GcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: EC transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Aminomethyltransferase HAMAP MF_00259		PRO_0000122603

Sequences

	Sequence		Length	Mass (Da)
	Q82JI2 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: DAAEC8E02547176F	FASTA	372	39,087
	10 20 30 40 50 60 MSSNAPRHTA LDALHRSLGA TMTDFAGWDM PLRYGSERDE HLAVRSKAGL FDLSHMGEIT 70 80 90 100 110 120 VTGPGAAALL NYALVGNIAS VGVGRARYTM ICRADGGILD DLIVYRLQEQ TYLVVANASN 130 140 150 160 170 180 AQVVLDALTE RAGGFDAVVR DDRDAYALIA VQGPESPGIL KSLTDADLDG LKYYAGLPGT 190 200 210 220 230 240 VAGVPALIAR TGYTGEDGFE LFVDPADAEK LWQALTEAGA PAGLVPCGLS CRDTLRLEAG 250 260 270 280 290 300 MPLYGHELST SLTPFDAGLG RVVKFEKEGD FVGREALTEA AALAEKNPPR VLVGLIAEGR 310 320 330 340 350 360 RVPRAGYPVV VGGEVIGEVT SGAPSPTLGR PIAMAYVDAA HAAPGTAGVG VDIRGSHEPY 370 EVVALPFYRR QK

References

[1]

"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites." Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed: 11572948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

[2]

"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis." Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. Nat. Biotechnol. 21:526-531(2003) [PubMed: 12692562] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000030 Genomic DNA. Translation: BAC70484.1.
RefSeq	NP_823949.1. NC_003155.4.
3D structure databases
ProteinModelPortal	Q82JI2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1210547.
GenomeReviews	Gene locus SAV_2773 in contig BA000030_GR.
KEGG	sma:SAV_2773.
NMPDR	fig|227882.1.peg.2775.
PATRIC	23719117. VBIStrAve112782_2968.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG299834.
OMA	KALYGGM.
ProtClustDB	PRK00389.
Enzyme and pathway databases
BioCyc	SAVE227882:SAV2773-MONOMER.
Family and domain databases
HAMAP	MF_00259. GcvT. [Tree]
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. IPR022903. NH2_Me_Trfase. [Graphical view]
KO	K00605.
PANTHER	PTHR13847:SF5. PTHR13847:SF5. 1 hit.
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. GcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_STRAW

Accession

Primary (citable) accession number: Q82JI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program