Reviewed, UniProtKB/Swiss-Prot Q82JF0 (GLGB1_STRAW)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme 1
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme 1
      Short name=BE 1
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 1

Gene names

Name:	glgB1
Ordered Locus Names:	SAV_2805

Organism

Streptomyces avermitilis [Complete proteome] [HAMAP]

Taxonomic identifier

33903 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	838 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 838	838	1,4-alpha-glucan-branching enzyme 1 HAMAP MF_00685		PRO_0000188747
Sites
	Active site	408	1	By similarity
	Active site	443	1	By similarity
	Active site	448	1	By similarity
	Active site	511	1	By similarity
	Active site	513	1	By similarity
	Active site	566	1	By similarity
	Active site	633	1	By similarity
	Active site	634	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q82JF0-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 3BFDF6B6AD3869CE	FASTA	838	92,740
	10 20 30 40 50 60 MIPRPPSDDR ANQNGDGSKK TGAKKTGAKK AAAAKKTAGK KATPAAKATA AKGAVTKKTG 70 80 90 100 110 120 KGKATAKKAV ADKAAAKATV PQQAVPKQAV SKRAVSKKAV PRKAVVKAAL DIPEAPVSPA 130 140 150 160 170 180 VAPDDRDRLL SGTHHAPHSV LGAHPVPGGV AFRVLRPYAL SVTVVTDDLR TELHDDGAGF 190 200 210 220 230 240 FTGLLPLRAV PDYRLHVAYE GTVHETEDAY RFLPALGELD LHLINEGRHE ELWTALGAEP 250 260 270 280 290 300 MTHQGVPGTR FTVWAPNARG VRLAGTFNFW DATAFPLRSL GSSGVWELFV PGVGEGELYK 310 320 330 340 350 360 FEITRPDGSK TLRADPVARR TEAPPRTSSI VHASHYAWAD EAWMAARGER PVHESPFSVY 370 380 390 400 410 420 EVHLPSWRPG LTYRQLAEQL PAYVADLGFT HVELLPVAEH PFGGSWGYQV TGFYAPTARL 430 440 450 460 470 480 GTPDDFKYLV DALHRAGVGV LMDWVPAHFP RDDWALAEFD GRPLYEHEDP LRAAHPDWGT 490 500 510 520 530 540 LEFDYGRREV RNFLVANAVY WCEEFHIDGL RVDAVASMLY LDYSREEGQW SPNEFGGREN 550 560 570 580 590 600 LDAVAFLQEM NATVYRRVPG VVTIAEESTA WEGVTRATHD NGLGFGLKWN MGWMHDSLGY 610 620 630 640 650 660 VQHEPVHRRF HHHEMTFSMV YAYSENYVLP ISHDEVVHGK GSLVSKMPGD WWQQRATERA 670 680 690 700 710 720 YLGFMWAHPG KQLLFMGQEF AQGAEWSETH GPDWWLLDPA YGAEPDHRGM RDLVRDLNTV 730 740 750 760 770 780 YRHEPALWER DTDPSGFAWV TGDAVEDNVF AFLRHAADGT PLLAVSNFSP VVRHDYRLGV 790 800 810 820 830 PDDIPAWHET LNTDGARYGG SDVTNPHPVK PEPQGRHGRP ASIRLTLPPL STLWLRPA

References

[1]

"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites." Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed: 11572948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

[2]

"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis." Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. Nat. Biotechnol. 21:526-531(2003) [PubMed: 12692562] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000030 Genomic DNA. Translation: BAC70516.1.
RefSeq	NP_823981.1.
3D structure databases
SMR	Q82JF0. Positions 229-837.
ModBase	Search...
Genome annotation databases
GeneID	1210554.
GenomeReviews	Gene locus SAV_2805 in contig BA000030_GR.
KEGG	sma:SAV_2805.
NMPDR	fig|227882.1.peg.2807.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287139.
OMA	NIRSEAC.
Enzyme and pathway databases
BioCyc	SAVE227882:SAV2805-MONOMER.
BRENDA	2.4.1.18. 140873.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB1_STRAW

Accession

Primary (citable) accession number: Q82JF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)