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Q82JF0 (GLGB1_STRAW) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB 1

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 1
Alpha-(1->4)-glucan branching enzyme 1
Glycogen branching enzyme 1
Short name=BE 1
Gene names
Name:glgB1
Ordered Locus Names:SAV_2805
OrganismStreptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680) [Complete proteome] [HAMAP]
Taxonomic identifier227882 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length838 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 8388381,4-alpha-glucan branching enzyme GlgB 1 HAMAP-Rule MF_00685
PRO_0000188747

Sites

Active site5131Nucleophile By similarity
Active site5661Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q82JF0 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 3BFDF6B6AD3869CE

FASTA83892,740
        10         20         30         40         50         60 
MIPRPPSDDR ANQNGDGSKK TGAKKTGAKK AAAAKKTAGK KATPAAKATA AKGAVTKKTG 

        70         80         90        100        110        120 
KGKATAKKAV ADKAAAKATV PQQAVPKQAV SKRAVSKKAV PRKAVVKAAL DIPEAPVSPA 

       130        140        150        160        170        180 
VAPDDRDRLL SGTHHAPHSV LGAHPVPGGV AFRVLRPYAL SVTVVTDDLR TELHDDGAGF 

       190        200        210        220        230        240 
FTGLLPLRAV PDYRLHVAYE GTVHETEDAY RFLPALGELD LHLINEGRHE ELWTALGAEP 

       250        260        270        280        290        300 
MTHQGVPGTR FTVWAPNARG VRLAGTFNFW DATAFPLRSL GSSGVWELFV PGVGEGELYK 

       310        320        330        340        350        360 
FEITRPDGSK TLRADPVARR TEAPPRTSSI VHASHYAWAD EAWMAARGER PVHESPFSVY 

       370        380        390        400        410        420 
EVHLPSWRPG LTYRQLAEQL PAYVADLGFT HVELLPVAEH PFGGSWGYQV TGFYAPTARL 

       430        440        450        460        470        480 
GTPDDFKYLV DALHRAGVGV LMDWVPAHFP RDDWALAEFD GRPLYEHEDP LRAAHPDWGT 

       490        500        510        520        530        540 
LEFDYGRREV RNFLVANAVY WCEEFHIDGL RVDAVASMLY LDYSREEGQW SPNEFGGREN 

       550        560        570        580        590        600 
LDAVAFLQEM NATVYRRVPG VVTIAEESTA WEGVTRATHD NGLGFGLKWN MGWMHDSLGY 

       610        620        630        640        650        660 
VQHEPVHRRF HHHEMTFSMV YAYSENYVLP ISHDEVVHGK GSLVSKMPGD WWQQRATERA 

       670        680        690        700        710        720 
YLGFMWAHPG KQLLFMGQEF AQGAEWSETH GPDWWLLDPA YGAEPDHRGM RDLVRDLNTV 

       730        740        750        760        770        780 
YRHEPALWER DTDPSGFAWV TGDAVEDNVF AFLRHAADGT PLLAVSNFSP VVRHDYRLGV 

       790        800        810        820        830 
PDDIPAWHET LNTDGARYGG SDVTNPHPVK PEPQGRHGRP ASIRLTLPPL STLWLRPA 

« Hide

References

[1]"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites."
Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M.
Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.
[2]"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis."
Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S.
Nat. Biotechnol. 21:526-531(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000030 Genomic DNA. Translation: BAC70516.1.
RefSeqNP_823981.1. NC_003155.4.

3D structure databases

ProteinModelPortalQ82JF0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING227882.SAV_2805.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC70516; BAC70516; SAV_2805.
GeneID1210554.
KEGGsma:SAV_2805.
PATRIC23719187. VBIStrAve112782_3003.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMATEDAYRF.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycSAVE227882:GJU1-2826-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB1_STRAW
AccessionPrimary (citable) accession number: Q82JF0
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: June 1, 2003
Last modified: June 11, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries