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Protein

Glyceraldehyde-3-phosphate dehydrogenase 1

Gene

gap1

Organism
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.1 Publication

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.1 Publication

Enzyme regulationi

Resistant to pentalenolactone.1 Publication

Kineticsi

kcat is 33 sec(-1) with D-glyceraldehyde 3-phosphate.1 Publication

Manual assertion based on experiment ini

  1. KM=0.33 mM for D-glyceraldehyde 3-phosphate1 Publication

    Pathwayi: glycolysis

    This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.Curated
    Proteins known to be involved in the 5 steps of the subpathway in this organism are:
    1. Glyceraldehyde-3-phosphate dehydrogenase 1 (gap1), Glyceraldehyde-3-phosphate dehydrogenase 2 (gap2)
    2. Phosphoglycerate kinase (pgk)
    3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
    4. Enolase (eno)
    5. Pyruvate kinase (pykA2), Pyruvate kinase (pykA1)
    This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei35NADBy similarity1
    Binding sitei79NAD; via carbonyl oxygenBy similarity1
    Active sitei153NucleophileBy similarity1
    Sitei180Activates thiol group during catalysisBy similarity1
    Binding sitei183Glyceraldehyde 3-phosphateBy similarity1
    Binding sitei198Glyceraldehyde 3-phosphateBy similarity1
    Binding sitei234Glyceraldehyde 3-phosphateBy similarity1
    Binding sitei315NADBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi12 – 13NADBy similarity2

    GO - Molecular functioni

    • glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity Source: UniProtKB
    • NAD binding Source: UniProtKB
    • NADP binding Source: InterPro

    GO - Biological processi

    • glucose metabolic process Source: InterPro
    • glycolytic process Source: UniProtKB-UniPathway
    • oxidation-reduction process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    NAD, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00184.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase 11 Publication (EC:1.2.1.121 Publication)
    Short name:
    GAPDH 11 Publication
    Alternative name(s):
    NAD-dependent glyceraldehyde-3-phosphate dehydrogenase1 Publication
    PL-insensitive glyceraldehyde-3-phosphate dehydrogenase1 Publication
    Gene namesi
    Name:gap1
    Ordered Locus Names:SAV_2990
    OrganismiStreptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
    Taxonomic identifieri227882 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces
    Proteomesi
    • UP000000428 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004220141 – 334Glyceraldehyde-3-phosphate dehydrogenase 1Add BLAST334

    Proteomic databases

    PRIDEiQ82IZ2.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi227882.SAV_2990.

    Structurei

    3D structure databases

    ProteinModelPortaliQ82IZ2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni152 – 154Glyceraldehyde 3-phosphate bindingBy similarity3
    Regioni211 – 212Glyceraldehyde 3-phosphate bindingBy similarity2

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105C17. Bacteria.
    COG0057. LUCA.
    HOGENOMiHOG000071679.
    KOiK00134.
    OMAiMAHLLAY.
    OrthoDBiPOG091H02BL.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR020831. GlycerAld/Erythrose_P_DH.
    IPR020830. GlycerAld_3-P_DH_AS.
    IPR020829. GlycerAld_3-P_DH_cat.
    IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
    IPR006424. Glyceraldehyde-3-P_DH_1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR10836. PTHR10836. 1 hit.
    PfamiPF02800. Gp_dh_C. 1 hit.
    PF00044. Gp_dh_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000149. GAP_DH. 1 hit.
    PRINTSiPR00078. G3PDHDRGNASE.
    SMARTiSM00846. Gp_dh_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51735. SSF51735. 1 hit.
    TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
    PROSITEiPS00071. GAPDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q82IZ2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTVRVGINGF GRIGRNVFRA AATRGADLEI VAVNDLGDVA TMAHLLAYDS
    60 70 80 90 100
    ILGRFPEEVT AEPGAIRAGD TTVKVLAERD PAALPWGDLG VDVVIESTGI
    110 120 130 140 150
    FTDAAKARAH VDGGAKKVII AAPASNEDVT VVLGVNQDAY DPERHTIISN
    160 170 180 190 200
    ASCTTNCLGV LAKVLHDAVG IESGMMTTVH AYTQDQNLQD APHKDLRRAR
    210 220 230 240 250
    AAGLNIVPTS SGAAKAIGLV LPELQGRLDA FALRVPVPTG SVTDLTVTAS
    260 270 280 290 300
    RSTTVEEVKE AYAKAAAGAY KGLLSYTEAP IVSTDIAGDP ASCVFDAELT
    310 320 330
    RVLGSQVKVV GWYDNEWGYS NRLIDLALLV GDTL
    Length:334
    Mass (Da):35,005
    Last modified:June 1, 2003 - v1
    Checksum:iCB7733D023257571
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BA000030 Genomic DNA. Translation: BAC70701.1.
    RefSeqiWP_010984421.1. NZ_JZJK01000090.1.

    Genome annotation databases

    EnsemblBacteriaiBAC70701; BAC70701; SAVERM_2990.
    KEGGisma:SAV_2990.
    PATRICi23719575. VBIStrAve112782_3196.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BA000030 Genomic DNA. Translation: BAC70701.1.
    RefSeqiWP_010984421.1. NZ_JZJK01000090.1.

    3D structure databases

    ProteinModelPortaliQ82IZ2.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi227882.SAV_2990.

    Proteomic databases

    PRIDEiQ82IZ2.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiBAC70701; BAC70701; SAVERM_2990.
    KEGGisma:SAV_2990.
    PATRICi23719575. VBIStrAve112782_3196.

    Phylogenomic databases

    eggNOGiENOG4105C17. Bacteria.
    COG0057. LUCA.
    HOGENOMiHOG000071679.
    KOiK00134.
    OMAiMAHLLAY.
    OrthoDBiPOG091H02BL.

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00184.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR020831. GlycerAld/Erythrose_P_DH.
    IPR020830. GlycerAld_3-P_DH_AS.
    IPR020829. GlycerAld_3-P_DH_cat.
    IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
    IPR006424. Glyceraldehyde-3-P_DH_1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR10836. PTHR10836. 1 hit.
    PfamiPF02800. Gp_dh_C. 1 hit.
    PF00044. Gp_dh_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000149. GAP_DH. 1 hit.
    PRINTSiPR00078. G3PDHDRGNASE.
    SMARTiSM00846. Gp_dh_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51735. SSF51735. 1 hit.
    TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
    PROSITEiPS00071. GAPDH. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiG3P1_STRAW
    AccessioniPrimary (citable) accession number: Q82IZ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2013
    Last sequence update: June 1, 2003
    Last modified: November 2, 2016
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.