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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei80SubstrateUniRule annotation1
Metal bindingi231ManganeseUniRule annotation1
Metal bindingi251Manganese; via tele nitrogenUniRule annotation1
Binding sitei273SubstrateUniRule annotation1
Active sitei275UniRule annotation1
Metal bindingi298ManganeseUniRule annotation1
Binding sitei390GTPUniRule annotation1
Binding sitei421GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi274 – 279GTPUniRule annotation6
Nucleotide bindingi516 – 519GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processGluconeogenesis
LigandGTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAVE227882:G1G23-3458-MONOMER
UniPathwayiUPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Synonyms:pck
Ordered Locus Names:SAV_3287
OrganismiStreptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
Taxonomic identifieri227882 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces
Proteomesi
  • UP000000428 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001036141 – 607Phosphoenolpyruvate carboxykinase [GTP]Add BLAST607

Proteomic databases

PRIDEiQ82I71

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi227882.SAV_3287

Structurei

3D structure databases

ProteinModelPortaliQ82I71
SMRiQ82I71
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni222 – 224Substrate bindingUniRule annotation3
Regioni388 – 390Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0M Bacteria
COG1274 LUCA
HOGENOMiHOG000191700
KOiK01596
OMAiCWLALTD
OrthoDBiPOG091H0B4F

Family and domain databases

CDDicd00819 PEPCK_GTP, 1 hit
Gene3Di3.40.449.10, 2 hits
3.90.228.20, 2 hits
HAMAPiMF_00452 PEPCK_GTP, 1 hit
InterProiView protein in InterPro
IPR018091 PEP_carboxykin_GTP_CS
IPR013035 PEP_carboxykinase_C
IPR008209 PEP_carboxykinase_GTP
IPR035077 PEP_carboxykinase_GTP_C
IPR035078 PEP_carboxykinase_GTP_N
IPR008210 PEP_carboxykinase_N
PANTHERiPTHR11561 PTHR11561, 1 hit
PfamiView protein in Pfam
PF00821 PEPCK_C, 1 hit
PF17297 PEPCK_N, 1 hit
PIRSFiPIRSF001348 PEP_carboxykinase_GTP, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit
PROSITEiView protein in PROSITE
PS00505 PEPCK_GTP, 1 hit

Sequencei

Sequence statusi: Complete.

Q82I71-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARDIAAPSS VPTQHKELIS WVNEIAELTQ PDSVVWCDGS EAEYERLSEE
60 70 80 90 100
LVRKGTFRKL DPIKRPNSYY AASDPTDVAR VEDRTFICSE KEEDAGPTNH
110 120 130 140 150
WKDPAEMREI FAGEQGVFRG SMRGRTMYVV PFCMGPLGSD LSAIGVEITD
160 170 180 190 200
SAYVAVSMRT MTRMGQPVLD ELGTDGFFVR AVHTLGAPLE AGEADVPWPC
210 220 230 240 250
NSTKYISHFP ESREIWSYGS GYGGNALLGK KCYALRIASV MARDEGWLAE
260 270 280 290 300
HMLILKLTPP QGESKYVAAA FPSACGKTNL AMLEPTVGGW TVETIGDDIA
310 320 330 340 350
WMRFGEDGRL YAINPEAGFF GVAPGTGEHT NANAMKTLWG NSVFTNVALT
360 370 380 390 400
DDNDIWWEGM TEETPAHLTD WKGNDWTPES GTPAAHPNAR FTVPASQCPI
410 420 430 440 450
IAPEWEDPKG VPISAILFGG RRATAVPLVT ESFDWNHGVF LGANVASEKT
460 470 480 490 500
AAAEGKVGEL RRDPFAMLPF CGYNMGDYMG HWVDVAKDKD QAKLPKIYYV
510 520 530 540 550
NWFRKNDEGK FVWPGFGENS RVLKWIVERL EGRAEGVETP IGILPTREAL
560 570 580 590 600
DTAGLDLSAS DLDFLLTVDK EVWREEAALV PEHLNTFGEH TPKELWDEYR

ALVQRLG
Length:607
Mass (Da):67,145
Last modified:June 1, 2003 - v1
Checksum:iB27FE1BFD41D50A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000030 Genomic DNA Translation: BAC70998.1
RefSeqiWP_010984717.1, NZ_JZJK01000090.1

Genome annotation databases

EnsemblBacteriaiBAC70998; BAC70998; SAVERM_3287
KEGGisma:SAVERM_3287

Similar proteinsi

Entry informationi

Entry nameiPCKG_STRAW
AccessioniPrimary (citable) accession number: Q82I71
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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