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Q82I71

- PCKG_STRAW

UniProt

Q82I71 - PCKG_STRAW

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Protein
Phosphoenolpyruvate carboxykinase [GTP]
Gene
pckG, pck, SAV_3287
Organism
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei80 – 801Substrate By similarity
Binding sitei224 – 2241Substrate; via amide nitrogen By similarity
Metal bindingi231 – 2311Manganese By similarity
Binding sitei231 – 2311Substrate By similarity
Metal bindingi251 – 2511Manganese; via tele nitrogen By similarity
Binding sitei273 – 2731Substrate By similarity
Active sitei275 – 2751 By similarity
Metal bindingi298 – 2981Manganese By similarity
Binding sitei390 – 3901GTP By similarity
Binding sitei421 – 4211GTP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi274 – 2796GTP By similarity
Nucleotide bindingi516 – 5194GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAVE227882:GJU1-3311-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
Short name:
PEP carboxykinase
Short name:
PEPCK
Gene namesi
Name:pckG
Synonyms:pck
Ordered Locus Names:SAV_3287
OrganismiStreptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680)
Taxonomic identifieri227882 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces
ProteomesiUP000000428: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 607607Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation
PRO_0000103614Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi227882.SAV_3287.

Structurei

3D structure databases

ProteinModelPortaliQ82I71.
SMRiQ82I71. Positions 11-607.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni388 – 3903Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiGNNIFTN.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q82I71-1 [UniParc]FASTAAdd to Basket

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MARDIAAPSS VPTQHKELIS WVNEIAELTQ PDSVVWCDGS EAEYERLSEE    50
LVRKGTFRKL DPIKRPNSYY AASDPTDVAR VEDRTFICSE KEEDAGPTNH 100
WKDPAEMREI FAGEQGVFRG SMRGRTMYVV PFCMGPLGSD LSAIGVEITD 150
SAYVAVSMRT MTRMGQPVLD ELGTDGFFVR AVHTLGAPLE AGEADVPWPC 200
NSTKYISHFP ESREIWSYGS GYGGNALLGK KCYALRIASV MARDEGWLAE 250
HMLILKLTPP QGESKYVAAA FPSACGKTNL AMLEPTVGGW TVETIGDDIA 300
WMRFGEDGRL YAINPEAGFF GVAPGTGEHT NANAMKTLWG NSVFTNVALT 350
DDNDIWWEGM TEETPAHLTD WKGNDWTPES GTPAAHPNAR FTVPASQCPI 400
IAPEWEDPKG VPISAILFGG RRATAVPLVT ESFDWNHGVF LGANVASEKT 450
AAAEGKVGEL RRDPFAMLPF CGYNMGDYMG HWVDVAKDKD QAKLPKIYYV 500
NWFRKNDEGK FVWPGFGENS RVLKWIVERL EGRAEGVETP IGILPTREAL 550
DTAGLDLSAS DLDFLLTVDK EVWREEAALV PEHLNTFGEH TPKELWDEYR 600
ALVQRLG 607
Length:607
Mass (Da):67,145
Last modified:June 1, 2003 - v1
Checksum:iB27FE1BFD41D50A5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000030 Genomic DNA. Translation: BAC70998.1.
RefSeqiNP_824463.1. NC_003155.4.

Genome annotation databases

EnsemblBacteriaiBAC70998; BAC70998; SAV_3287.
GeneIDi1210703.
KEGGisma:SAV_3287.
PATRICi23720211. VBIStrAve112782_3512.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000030 Genomic DNA. Translation: BAC70998.1 .
RefSeqi NP_824463.1. NC_003155.4.

3D structure databases

ProteinModelPortali Q82I71.
SMRi Q82I71. Positions 11-607.
ModBasei Search...

Protein-protein interaction databases

STRINGi 227882.SAV_3287.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC70998 ; BAC70998 ; SAV_3287 .
GeneIDi 1210703.
KEGGi sma:SAV_3287.
PATRICi 23720211. VBIStrAve112782_3512.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
KOi K01596.
OMAi GNNIFTN.
OrthoDBi EOG6MPWQS.

Enzyme and pathway databases

UniPathwayi UPA00138 .
BioCyci SAVE227882:GJU1-3311-MONOMER.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites."
    Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M.
    Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.
  2. "Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis."
    Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S.
    Nat. Biotechnol. 21:526-531(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.

Entry informationi

Entry nameiPCKG_STRAW
AccessioniPrimary (citable) accession number: Q82I71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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