Q82HU1 (SYW1_STRAW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan--tRNA ligase 1
EC=6.1.1.2

Alternative name(s):
Tryptophanyl-tRNA synthetase 1
Short name=TrpRS 1

Gene names

Name:	trpS1
Ordered Locus Names:	SAV_3417

Organism

Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680) [Complete proteome] [HAMAP]

Taxonomic identifier

227882 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces › Streptomyces avermitilis

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tryptophanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP tryptophan-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Tryptophan--tRNA ligase 1 HAMAP-Rule MF_00140		PRO_0000136685
Regions
	Motif	14 – 23	10	"HIGH" region HAMAP-Rule MF_00140
	Motif	199 – 203	5	"KMSKS" region HAMAP-Rule MF_00140
Sites
	Binding site	202	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q82HU1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 23DAF4D121131864	FASTA	337	37,096
	10 20 30 40 50 60 MASDRPRVLS GIQPTAGSFH LGNYLGAVRQ WVALQESHDA FYMVVDLHAI TVPQDPADLR 70 80 90 100 110 120 ANTRLAAAQL LAAGLDPERC TLFVQSHVPE HAQLAWIMNC LTGFGEASRM TQFKDKSAKQ 130 140 150 160 170 180 GADRASVGLF TYPVLQVADI LLYQANEVPV GEDQRQHIEL TRDLAERFNG RFGETFTVPK 190 200 210 220 230 240 PYILKETAKI FDLQDPSIKM SKSASTPKGL INLLDEPKAT AKKVKSAVTD TDTVIRYDAE 250 260 270 280 290 300 HKPGISNLLT IYSTLTGTGI PELEEKYTGK GYGALKTDLA EVMVDFVTPF RERTQQYLDD 310 320 330 PETLDSILAK GAEKARAVAA ETLSQAYDRV GFLPAKH

References

[1]

"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites." Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.

[2]

"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis." Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. Nat. Biotechnol. 21:526-531(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000030 Genomic DNA. Translation: BAC71129.1.
RefSeq	NP_824594.1. NC_003155.4.
3D structure databases
ProteinModelPortal	Q82HU1.
SMR	Q82HU1. Positions 6-333.
ModBase	Search...
Protein-protein interaction databases
STRING	227882.SAV_3417.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC71129; BAC71129; SAV_3417.
GeneID	1210736.
KEGG	sma:SAV_3417.
PATRIC	23720487. VBIStrAve112782_3650.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0180.
HOGENOM	HOG000059940.
KO	K01867.
OMA	HDCLYCV.
ProtClustDB	PRK00927.
Enzyme and pathway databases
BioCyc	SAVE227882:GJU1-3442-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00140_B. Trp_tRNA_synth_B.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002306. Trp-tRNA-ligase. IPR024109. Trp-tRNA-ligase_bac-type. [Graphical view]
PANTHER	PTHR10055. PTHR10055. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01039. TRNASYNTHTRP.
TIGRFAMs	TIGR00233. trpS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYW1_STRAW

Accession

Primary (citable) accession number: Q82HU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program