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Protein

Malate dehydrogenase

Gene

mdh

Organism
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate. Exhibits remarkably higher catalytic efficiency for oxaloacetate reduction than for malate oxidation in vitro. Highly specific for NAD(H). Can also use NADPH for oxaloacetate reduction, but catalytic efficiency is 97-fold higher with NADH. No activity detected with NADP+ and malate.1 Publication

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation1 Publication

Enzyme regulationi

Strongly inhibited by Hg2+ and Zn2+. Activated by Na+, NH4+, Ca2+, Cu2+ and Mg2+.1 Publication

Kineticsi

kcat is 1181.6 sec(-1) for NADH-dependent reduction of oxaloacetate. kcat is 67.4 sec(-1) for NADPH-dependent reduction of oxaloacetate. kcat is 4.88 sec(-1) for NAD+-dependent oxidation of malate.1 Publication

Manual assertion based on experiment ini

  1. KM=75.5 µM for oxaloacetate (in the presence of NADH)1 Publication
  2. KM=433.6 µM for oxaloacetate (in the presence of NADPH)1 Publication
  3. KM=36.8 µM for NADH1 Publication
  4. KM=374.1 µM for NADPH1 Publication
  5. KM=386 µM for malate1 Publication
  6. KM=592 µM for NAD+1 Publication

    pH dependencei

    Optimum pH is 8.0.1 Publication

    Temperature dependencei

    Optimum temperature is 42 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei93SubstrateUniRule annotation1
    Binding sitei99SubstrateUniRule annotation1
    Binding sitei106NADUniRule annotation1
    Binding sitei113NADUniRule annotation1
    Binding sitei132SubstrateUniRule annotation1
    Binding sitei163SubstrateUniRule annotation1
    Active sitei188Proton acceptorUniRule annotation1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi12 – 18NADUniRule annotation7
    Nucleotide bindingi130 – 132NADUniRule annotation3

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase1 PublicationUniRule annotation (EC:1.1.1.37UniRule annotation1 Publication)
    Gene namesi
    Name:mdhUniRule annotation
    Ordered Locus Names:SAV_3436
    OrganismiStreptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
    Taxonomic identifieri227882 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces
    Proteomesi
    • UP000000428 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001133931 – 329Malate dehydrogenaseAdd BLAST329

    Interactioni

    Protein-protein interaction databases

    STRINGi227882.SAV_3436.

    Structurei

    3D structure databases

    ProteinModelPortaliQ82HS2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105D9Z. Bacteria.
    COG0039. LUCA.
    HOGENOMiHOG000220953.
    KOiK00024.
    OMAiQYPDAGQ.
    OrthoDBiPOG091H03R4.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_01517. Malate_dehydrog_2. 1 hit.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23382. PTHR23382. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF51735. SSF51735. 1 hit.
    SSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q82HS2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTRTPVNVTV TGAAGQIGYA LLFRIASGQL LGADVPVKLR LLEITPALKA
    60 70 80 90 100
    AEGTAMELDD CAFPLLQGID ITDDPNVAFD GTNVGLLVGA RPRTKGMERG
    110 120 130 140 150
    DLLSANGGIF KPQGKAINDN AADDVKILVV GNPANTNALI AQAAAPDVPA
    160 170 180 190 200
    ERFTAMTRLD HNRALTQLAK KTGSTVADIK RLTIWGNHSA TQYPDIFHAS
    210 220 230 240 250
    VAGKNAAEVV NDEKWLAEDF IPTVAKRGAA IIEARGASSA ASAANAAIDH
    260 270 280 290 300
    VYTWVNGTAD GDWTSMGIPS DGSYGVPEGL ISSFPVTTKD GRYEIVQGLE
    310 320
    INEFSRARID ASVKELEEER EAVRALGLI
    Length:329
    Mass (Da):34,689
    Last modified:June 1, 2003 - v1
    Checksum:iD68E4B4720651655
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BA000030 Genomic DNA. Translation: BAC71148.1.
    RefSeqiWP_010984867.1. NZ_JZJK01000090.1.

    Genome annotation databases

    EnsemblBacteriaiBAC71148; BAC71148; SAVERM_3436.
    KEGGisma:SAV_3436.
    PATRICi23720533. VBIStrAve112782_3673.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BA000030 Genomic DNA. Translation: BAC71148.1.
    RefSeqiWP_010984867.1. NZ_JZJK01000090.1.

    3D structure databases

    ProteinModelPortaliQ82HS2.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi227882.SAV_3436.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiBAC71148; BAC71148; SAVERM_3436.
    KEGGisma:SAV_3436.
    PATRICi23720533. VBIStrAve112782_3673.

    Phylogenomic databases

    eggNOGiENOG4105D9Z. Bacteria.
    COG0039. LUCA.
    HOGENOMiHOG000220953.
    KOiK00024.
    OMAiQYPDAGQ.
    OrthoDBiPOG091H03R4.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_01517. Malate_dehydrog_2. 1 hit.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23382. PTHR23382. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF51735. SSF51735. 1 hit.
    SSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMDH_STRAW
    AccessioniPrimary (citable) accession number: Q82HS2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2004
    Last sequence update: June 1, 2003
    Last modified: November 2, 2016
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.