Reviewed, UniProtKB/Swiss-Prot Q82GD0 (SYC1_STRAW)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase 1
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase 1
      Short name=CysRS 1

Gene names

Name:	cysS1
Ordered Locus Names:	SAV_3967

Organism

Streptomyces avermitilis [Complete proteome] [HAMAP]

Taxonomic identifier

33903 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00041
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Cysteinyl-tRNA synthetase 1 HAMAP MF_00041		PRO_0000159488
Regions
	Motif	31 – 41	11	"HIGH" region HAMAP MF_00041
	Motif	264 – 268	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	29	1	Zinc By similarity
	Metal binding	208	1	Zinc By similarity
	Metal binding	233	1	Zinc By similarity
	Metal binding	237	1	Zinc By similarity
	Binding site	267	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q82GD0-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 64B00ECC32961E23	FASTA	466	52,242
	10 20 30 40 50 60 MTIRLYDTSA RQIRDFTPLL PGCVSIYLCG ATVQAAPHIG HIRSGLNFDI MRRWFEYRGY 70 80 90 100 110 120 DVTFIRNVTD IDDKIIAKSA DHGRPWWAIG YENERAFNDG YDVLGCLPPT YEPRATGHIT 130 140 150 160 170 180 EMVEMMRTLI ERGHAYEADG NVYFDVRSFP EYLQLSNQEL DNLLQPSGEG ETGKRDPRDF 190 200 210 220 230 240 AMWKAAKPGE PTWETPWGRG RPGWHLECSA MAHKYLGSAF DIHGGGLDLI FPHHENEIAQ 250 260 270 280 290 300 AKAFGDEFAR YWVHNAWVTM SGEKMSKSLG NSVLVSEMVK QWRPIVLRYY LGTPHYRSMI 310 320 330 340 350 360 EYSEEALREA ESAFARIEGF VQRVVEKAGG VVEPSPEVPP AFAEAMDDDL GVPQALAIVH 370 380 390 400 410 420 TTVRQGNSAL AADDKEAAVA RLAEVRAMLG VLGLDPLDPQ WAGEGDRGED LHGVVDTLVR 430 440 450 460 MVLDQREAAR ARKDWATADA IRDQLNQSGL VIEDSPQGPR WTLGPR

References

[1]

"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites." Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed: 11572948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

[2]

"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis." Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. Nat. Biotechnol. 21:526-531(2003) [PubMed: 12692562] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000030 Genomic DNA. Translation: BAC71679.1.
RefSeq	NP_825144.1.
3D structure databases
HSSP	HSSP built from PDB template 1LI5 based on UniProtKB P21888.
ModBase	Search...
Genome annotation databases
GeneID	1210850.
GenomeReviews	Gene locus SAV_3967 in contig BA000030_GR.
KEGG	sma:SAV_3967.
NMPDR	fig|227882.1.peg.3970.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q82GD0.
OMA	VLWKAAK.
Enzyme and pathway databases
BioCyc	SAVE227882:SAV3967-MON.
BRENDA	6.1.1.16. 140873.
Family and domain databases
HAMAP	MF_00041. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC1_STRAW

Accession

Primary (citable) accession number: Q82GD0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)