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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 5311D-inositol 3-phosphateUniRule annotation
Binding sitei67 – 671UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei122 – 12211D-inositol 3-phosphateUniRule annotation
Binding sitei155 – 15511D-inositol 3-phosphateUniRule annotation
Binding sitei179 – 17911D-inositol 3-phosphateUniRule annotation
Binding sitei199 – 19911D-inositol 3-phosphateUniRule annotation
Binding sitei274 – 2741UDP-GlcNAcUniRule annotation
Binding sitei279 – 2791UDP-GlcNAcUniRule annotation
Binding sitei340 – 3401UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation
Metal bindingi349 – 3491Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi350 – 3501Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi352 – 3521Magnesium; via carbonyl oxygenUniRule annotation
Binding sitei362 – 3621UDP-GlcNAcUniRule annotation
Binding sitei370 – 3701UDP-GlcNAcUniRule annotation
Metal bindingi376 – 3761MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSAVE227882:GJU1-4036-MONOMER.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Synonyms:glgA1
Ordered Locus Names:SAV_4006
OrganismiStreptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
Taxonomic identifieri227882 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces
Proteomesi
  • UP000000428 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 464464D-inositol 3-phosphate glycosyltransferasePRO_0000400160Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi227882.SAV_4006.

Structurei

3D structure databases

ProteinModelPortaliQ82G92.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni59 – 602UDP-GlcNAc bindingUniRule annotation
Regioni64 – 6961D-inositol 3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q82G92-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGAPRRPDR HARSEEERHV SQYASRLGRR SPAAPTRRRM LRKPRRVAML
60 70 80 90 100
SVHTSPLHQP GTGDAGGMNV YIVELAQRLA AINIEVEIFT RATTAALRPT
110 120 130 140 150
VELSPGVLVR HVDAGPYEGL AKEDLPAQLC AFTHGVMQAW AGHRPGYYDL
160 170 180 190 200
VHSHYWLSGH VGWLAAQRWG TPLVHAMHTM AKVKNAALAE GDTPEPAARV
210 220 230 240 250
IGEMQIVAAA DRLIANTSEE ADELVRHYEA ERGKVAVVHP GVNLDRFRPA
260 270 280 290 300
DGRAAARARL GLPQDALIPL FAGRIQPLKA PDVLLRAVAV LLDERPELRS
310 320 330 340 350
NLVVPVVGGP SGSGLAKPEG LQKLAARLGI ADVVRFRPPV GQEQLADWFR
360 370 380 390 400
AASVLVMPSY NESFGLVAIE AQAAGTPVLA ASVGGLPVAV ADGRTGFLVQ
410 420 430 440 450
GHDPAAYARV LRDFADDPAL SARMGRAAAR HAECFGWDTA ASATADVYTA
460
AMQAHRRRVR SHHG
Length:464
Mass (Da):49,980
Last modified:June 1, 2003 - v1
Checksum:iF99139F1B4E01239
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000030 Genomic DNA. Translation: BAC71718.1.

Genome annotation databases

EnsemblBacteriaiBAC71718; BAC71718; SAV_4006.
KEGGisma:SAV_4006.
PATRICi23721731. VBIStrAve112782_4267.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000030 Genomic DNA. Translation: BAC71718.1.

3D structure databases

ProteinModelPortaliQ82G92.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi227882.SAV_4006.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC71718; BAC71718; SAV_4006.
KEGGisma:SAV_4006.
PATRICi23721731. VBIStrAve112782_4267.

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Enzyme and pathway databases

BioCyciSAVE227882:GJU1-4036-MONOMER.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHA_STRAW
AccessioniPrimary (citable) accession number: Q82G92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: June 1, 2003
Last modified: September 7, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.