Q82F68 (SYD_STRAW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	SAV_4395

Organism

Streptomyces avermitilis [Complete proteome] [HAMAP]

Taxonomic identifier

33903 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	587 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 587	587	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_0000110951

Sequences

	Sequence		Length	Mass (Da)
	Q82F68 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 377A27DAF9EC9823	FASTA	587	65,725
	10 20 30 40 50 60 MHRYRSHTCG ELRASDVGSD VRLSGWLHNR RDLGGILFID LRDHYGITQL VARPGTPAYE 70 80 90 100 110 120 ALDKVSKEST VRVDGKVVSR GTENVNPDLP TGEIEVEVSE VELLGAAAPL PFTINAEDGV 130 140 150 160 170 180 NEERRLEYRF LDLRRERMHR NILLRTAVIS AIRHKMTALG FNEMATPILS ATSPEGARDF 190 200 210 220 230 240 VVPSRLHPGK FYALPQAPQQ FKQLLMISGF DRYFQIAPCF RDEDARADRS PGEFYQLDVE 250 260 270 280 290 300 MSFVEQEDVF QPIEKLMTEL FEEFGGGRHV TSPFPRIPFR EAMLKYGSDK PDLRAQLELV 310 320 330 340 350 360 DITDIFEGSE FKAFAGKHVR ALPVPDVSGQ TRKFFDGLGD YAVEQGAKGL AWVRVGEDGS 370 380 390 400 410 420 LTGPIAKFLT EENVAELTKR LSLAPGHAVF FGAGEFDEVS KIMGAVRVEA AKRSGNFEEN 430 440 450 460 470 480 VFRFCWIVDF PMYEKDEDTG KIDFSHNPFS MPQGGLEALE TQDPLDILGW QYDIVCNGVE 490 500 510 520 530 540 LSSGAIRNHE PEIMLKAFEI AGYDRDTVEE QFAGMLRAFR FGAPPHGGIA PGVDRIVMLL 550 560 570 580 ADEPNIRETI AFPLNGNAQD LMMGAPTELD ETRLRELHLS VRKPQPK

References

[1]

"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites." Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed: 11572948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

[2]

"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis." Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. Nat. Biotechnol. 21:526-531(2003) [PubMed: 12692562] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000030 Genomic DNA. Translation: BAC72107.1.
RefSeq	NP_825572.1. NC_003155.4.
3D structure databases
ProteinModelPortal	Q82F68.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1210986.
GenomeReviews	Gene locus SAV_4395 in contig BA000030_GR.
KEGG	sma:SAV_4395.
NMPDR	fig|227882.1.peg.4398.
PATRIC	23722595. VBIStrAve112782_4679.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG396032.
OMA	YQLDVEM.
ProtClustDB	PRK00476.
Enzyme and pathway databases
BioCyc	SAVE227882:SAV4395-MONOMER.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_STRAW

Accession

Primary (citable) accession number: Q82F68

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program