Q82EL5 (ACSA_STRAW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-coenzyme A synthetase
EC=6.2.1.1

Alternative name(s):
Acetate--CoA ligase
Acyl-activating enzyme

Gene names

Name:	acsA
Ordered Locus Names:	SAV_4599

Organism

Streptomyces avermitilis [Complete proteome] [HAMAP]

Taxonomic identifier

33903 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	652 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity. HAMAP MF_01123
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 652	652	Acetyl-coenzyme A synthetase HAMAP MF_01123		PRO_0000208388
Sites
	Active site	518	1	By similarity
Amino acid modifications
	Modified residue	611	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q82EL5 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: F9834F4332A3279A	FASTA	652	71,091
	10 20 30 40 50 60 MSNESLANLL KEERRFAPPA DLAANANVTA EAYEQAKADR LGFWAEQARR LTWATEPTET 70 80 90 100 110 120 LDWSNPPFAK WFKDGKLNVA YNCVDRHVEA GHGDRVAIHF EGEPGDSRAI TYAELKDEVS 130 140 150 160 170 180 KAANALTELG VQKGDRVAVY LPMIPEAVVA MLACARIGAA HSVVFGGFSA DAIAARIKDA 190 200 210 220 230 240 DAKLVITADG GYRRGKPSAL KPAVDDAVSR GDGVEKVLVV RRTGQEVAWT EGRDVWWHEI 250 260 270 280 290 300 TAKQSAEHTP EAFDAEHPLF ILYTSGTTGK PKGILHTSGG YLTQTSYTHH AVFDLKPETD 310 320 330 340 350 360 VYWCTADIGW VTGHSYITYG PLSNGATQVM YEGTPDTPHQ GRFWEIVQKY GVTILYTAPT 370 380 390 400 410 420 AIRTFMKWGD DIPAKFDLSS LRVLGSVGEP INPEAWIWYR KHIGGDRTPI VDTWWQTETG 430 440 450 460 470 480 AMMISPLPGV TETKPGSAQR PLPGISATVV DDEAREVPNG GGGYLVLTEP WPSMLRTIWG 490 500 510 520 530 540 DDQRFLDTYW SRFEGKYFAG DGAKKDEDGD IWLLGRVDDV MLVSGHNIST TEVESALVSH 550 560 570 580 590 600 PSVAEAAVVG AADETTGQAI VAFVILRGTA NAEDDNLVAD LRNHVGTTLG PIAKPKRILP 610 620 630 640 650 VAELPKTRSG KIMRRLLRDV AENRALGDVT TLTDSSVMDL IQSKLPAAPS ED

References

[1]

"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites." Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed: 11572948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

[2]

"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis." Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. Nat. Biotechnol. 21:526-531(2003) [PubMed: 12692562] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000030 Genomic DNA. Translation: BAC72311.1.
RefSeq	NP_825776.1. NC_003155.4.
3D structure databases
ProteinModelPortal	Q82EL5.
SMR	Q82EL5. Positions 15-644.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1211019.
GenomeReviews	Gene locus SAV_4599 in contig BA000030_GR.
KEGG	sma:SAV_4599.
NMPDR	fig|227882.1.peg.4602.
PATRIC	23723073. VBIStrAve112782_4916.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG547964.
OMA	VISVSGH.
ProtClustDB	PRK00174.
Enzyme and pathway databases
BioCyc	SAVE227882:SAV4599-MONOMER.
Family and domain databases
HAMAP	MF_01123. Ac_CoA_synth. [Tree]
InterPro	IPR011904. Ac_CoA_lig. IPR024597. Acyl-CoA_synth_DUF3448. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
KO	K01895.
PANTHER	PTHR24095:SF42. PTHR24095:SF42. 1 hit.
Pfam	PF00501. AMP-binding. 1 hit. PF11930. DUF3448. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA_STRAW

Accession

Primary (citable) accession number: Q82EL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program