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Reviewed, UniProtKB/Swiss-Prot Q82EJ8 (HPPA_STRAW)

Last modified November 3, 2009. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Pyrophosphate-energized proton pump
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate-energized inorganic pyrophosphatase
      Short name=H(+)-PPase
    Membrane-bound proton-translocating pyrophosphatase
Gene names
Name: hppA
Ordered Locus Names: SAV_4616
OrganismStreptomyces avermitilis [Complete proteome] [HAMAP]
Taxonomic identifier33903 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

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Protein attributes

Sequence length801 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Generates a proton motive force; it probably catalyzes a fully reversible reaction, thus being able to synthesize pyrophosphate when the proton motive force is sufficient By similarity.

Catalytic activity

Diphosphate + H2O = 2 phosphate. HAMAP MF_01130

Cofactor

Magnesium By similarity.

Subunit structure

Homodimer Potential.

Subcellular location

Cell membrane; Multi-pass membrane protein Probable.

Sequence similarities

Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. Type 2 subfamily. [View classification]

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Ontologies

Keywords
   Biological processHydrogen ion transport
Ion transport
Transport
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
   LigandMagnesium
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processproton transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Inferred from electronic annotation. Source: HAMAP

   Molecular functionhydrogen-translocating pyrophosphatase activity

Inferred from electronic annotation. Source: InterPro

inorganic diphosphatase activity

Inferred from electronic annotation. Source: HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 801801Pyrophosphate-energized proton pump HAMAP MF_01130
PRO_0000217031

Regions

Transmembrane28 – 4720 Potential
Transmembrane81 – 9818 Potential
Transmembrane108 – 13023 Potential
Transmembrane171 – 19323 Potential
Transmembrane198 – 22023 Potential
Transmembrane267 – 28620 Potential
Transmembrane291 – 31323 Potential
Transmembrane326 – 34823 Potential
Transmembrane372 – 39423 Potential
Transmembrane414 – 43623 Potential
Transmembrane451 – 47323 Potential
Transmembrane569 – 59123 Potential
Transmembrane641 – 66323 Potential
Transmembrane722 – 74423 Potential
Transmembrane754 – 77320 Potential

Sites

Site5141Determinant of potassium independence By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q82EJ8-1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 3039F19A8AB6E289

FASTA80181,610
        10         20         30         40         50         60 
MAGLSTPHQF DHPTALAAAV LTDDNRVIVM VIGVVALAAL VVAGILVRQV LAAGEGTDSM 

        70         80         90        100        110        120 
KKIAAAVQEG ANAYLGRQMR TLGVFAVVVF FLLMLLPADD WNQRAGRSVF FLIGALFSAT 

       130        140        150        160        170        180 
TGYTGMWLAV RSNVRVAAAA REATPAEGEP EKDLTAVSHK AMKIAFRTGG VVGMFTVGLG 

       190        200        210        220        230        240 
LLGASCVVLV YAADAPKVLE GFGLGAALIA MFMRVGGGIF TKAADVGADL VGKVEQGIPE 

       250        260        270        280        290        300 
DDPRNAATIA DNVGDNVGDC AGMAADLFES YAVTLVAALI LGKAAFGDSG LAFPLIVPAI 

       310        320        330        340        350        360 
GVLTAMIGIF AVAPRRADRS GMSAINRGFF VSAVFSLALV AVAVYVYLPG KYADLDGVTD 

       370        380        390        400        410        420 
VAIRAKDGDP RILAMVAVAI GIVLAALIQQ LTGYFTETTR RPVRDIGKSS LTGAATVVLA 

       430        440        450        460        470        480 
GISVGLESAV YTALLIGLGV YGAFLLGGTS IMLALFAVAL AGTGLLTTVG VIVAMDTFGP 

       490        500        510        520        530        540 
VSDNAQGIAE MSGDVTGAGA QVLTDLDAVG NTTKAITKGI AIATAVLAAS ALFGSYRDAI 

       550        560        570        580        590        600 
TTAANDVGEK VSGAGAPMNL MMDISQPNNL VGLIAGAAVV FLFSGLAINA VSRSAGAVVY 

       610        620        630        640        650        660 
EVRRQFREHP GIMDYTEQPE YGRVVDICTK DALRELATPG LLAVLAPIAI GFTLGVGALG 

       670        680        690        700        710        720 
AYLAGAIGTG TLMAVFLANS GGAWDNAKKL VEDGHHGGKG SEAHAATVIG DTVGDPFKDT 

       730        740        750        760        770        780 
AGPAINPLLK VMNLVALLIA PAVVKFSYGE DKNLGVRIAI AVLSILVIVG AVYISKRRGI 

       790        800 
AVGDEGNAER VTKSADPAVV S

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References

[1]"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites."
Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M.
Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed: 11572948] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.
[2]"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis."
Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S.
Nat. Biotechnol. 21:526-531(2003) [PubMed: 12692562] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

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Cross-references

Sequence databases

BA000030 Genomic DNA. Translation: BAC72328.1.
RefSeqNP_825793.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID1215410.
GenomeReviewsGene locus SAV_4616 in contig BA000030_GR.
KEGGsma:SAV_4616.
NMPDRfig|227882.1.peg.4619.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ82EJ8.
OMAPYLFGGI.

Enzyme and pathway databases

BioCycSAVE227882:SAV4616-MON.
BRENDA3.6.1.1. 140873.

Family and domain databases

HAMAPMF_01130.
[Tree]
InterProIPR004131. H_PPase.
[Graphical view]
PfamPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsTIGR01104. V_PPase. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameHPPA_STRAW
AccessionPrimary (citable) accession number: Q82EJ8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: June 1, 2003
Last modified: November 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents