Q82E74 (Q82E74_STRAW) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Delta-aminolevulinic acid dehydratase RuleBase RU000515
EC=4.2.1.24 RuleBase RU000515

Gene names

Name:	hemB EMBL BAC72454.1
Ordered Locus Names:	SAV_4742 EMBL BAC72454.1

Organism

Streptomyces avermitilis [Complete proteome] [HAMAP]

Taxonomic identifier

33903 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 5-aminolevulinate = porphobilinogen + 2 H2O. RuleBase RU000515
Subunit structure	Homooctamer By similarity. RuleBase RU000515
Sequence similarities	Belongs to the ALADH family. RuleBase RU004161

Ontologies

Keywords
Biological process	Porphyrin biosynthesis RuleBase RU000515
Ligand	Magnesium PIRSR PIRSR001415-5 Metal-binding PIRSR PIRSR001415-5
Molecular function	Lyase RuleBase RU000515
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW porphobilinogen synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	203	1	Schiff-base intermediate with substrate By similarity PIRSR PIRSR001415-1
	Active site	255	1	Schiff-base intermediate with substrate By similarity PIRSR PIRSR001415-1
	Metal binding	240	1	Magnesium By similarity PIRSR PIRSR001415-5
	Binding site	213	1	Substrate 1 By similarity PIRSR PIRSR001415-2
	Binding site	224	1	Substrate 1 By similarity PIRSR PIRSR001415-2
	Binding site	281	1	Substrate 2 By similarity PIRSR PIRSR001415-2
	Binding site	320	1	Substrate 2 By similarity PIRSR PIRSR001415-2

Sequences

	Sequence		Length	Mass (Da)
	Q82E74 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 654A9DBD23EFD2DB	FASTA	330	35,618
	10 20 30 40 50 60 MTKYGSFPGT RPRRLRTTPA LRRMVAETRL HPADFILPAF VREGVSEPVP ITAMPGVVQH 70 80 90 100 110 120 TRDSLKKAAL EALEAGVSGI MLFGVPEDAK KDAQGSAGTD PDGILQVALR DVRAEVGDEL 130 140 150 160 170 180 IVMSDLCLDE FTDHGHCGVL DGQGRVDNDA TLERYAEMAQ VQADAGAHVV GPSGMMDGQI 190 200 210 220 230 240 GVVRDALDQI GREDVSILAY TAKYSSAFYG PFREAVGSSL KGDRKTYQQD PANARESLRE 250 260 270 280 290 300 LALDLEEGAD MVMVKPAGPY LDILARVADA VDVPLAAYQI SGEYSMIEAA AEKGWIDRDK 310 320 330 AILETLTGIK RAGAQNILTY WATEVAQKLR

References

[1]

"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis." Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. Nat. Biotechnol. 21:526-531(2003) [PubMed: 12692562] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites." Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed: 11572948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000030 Genomic DNA. Translation: BAC72454.1.
RefSeq	NP_825919.1. NC_003155.4.
3D structure databases
HSSP	HSSP built from PDB template 1B4K based on UniProtKB Q59643.
ProteinModelPortal	Q82E74.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1211065.
GenomeReviews	Gene locus SAV4742 in contig BA000030_GR.
KEGG	sma:SAV_4742.
NMPDR	fig|227882.1.peg.4745.
PATRIC	23723367. VBIStrAve112782_5059.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285270.
OMA	FTSHGHD.
ProtClustDB	PRK09283.
Enzyme and pathway databases
BioCyc	SAVE227882:SAV4742-MONOMER.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR001731. Porphobilinogen_synth. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01698.
PANTHER	PTHR11458. AlaD_dehydratase. 1 hit.
Pfam	PF00490. ALAD. 1 hit. [Graphical view]
PIRSF	PIRSF001415. Porphbilin_synth. 1 hit.
PRINTS	PR00144. DALDHYDRTASE.
SMART	SM01004. ALAD. 1 hit. [Graphical view]
PROSITE	PS00169. D_ALA_DEHYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q82E74_STRAW

Accession

Primary (citable) accession number: Q82E74

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)