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Q82C66

- SYL_STRAW

UniProt

Q82C66 - SYL_STRAW

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei736 – 7361ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSAVE227882:GJU1-5564-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
    Alternative name(s):
    Leucyl-tRNA synthetaseUniRule annotation
    Short name:
    LeuRSUniRule annotation
    Gene namesi
    Name:leuSUniRule annotation
    Ordered Locus Names:SAV_5488
    OrganismiStreptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680)
    Taxonomic identifieri227882 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces
    ProteomesiUP000000428: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 962962Leucine--tRNA ligasePRO_0000152092Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi227882.SAV_5488.

    Structurei

    3D structure databases

    ProteinModelPortaliQ82C66.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi68 – 7912"HIGH" regionAdd
    BLAST
    Motifi733 – 7375"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0495.
    HOGENOMiHOG000200748.
    KOiK01869.
    OMAiLGMFPYT.
    OrthoDBiEOG63Z74X.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 4 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_00049_B. Leu_tRNA_synth_B.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q82C66-1 [UniParc]FASTAAdd to Basket

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    MSETNPAAAS EVAAPHRYTA AVAAEIEARW QDFWDAEGTY EAPNPTGDLA    50
    GGPALVAKPK KFVMDMFPYP SGAGLHVGHP LGYIATDVFA RFQRMTGHNV 100
    LHTLGFDAFG LPAEQYAVQT GTHPRVSTEA NIENMKVQLR RLGLGHDKRR 150
    SFATIDPDYY KWTQWIFLQI FNSWYDEEAN KARPIAELVA QFESGERAVP 200
    RADGATRAWR ELTAAERADV LGEYRLAYAS EAPVNWSPGL GTVLANEEVT 250
    ADGRSERGNF PVFKAKLRQW NMRITAYADR LLDDLDALDW PEAIKLQQRN 300
    WIGRSEGARV DFPIDGEAVT VFTTRPDTLF GATYMVLAPE HPLVEKFTPA 350
    AWPEGTHDVW TGGHATPAEA VAAYRAQAAS KSDVERQAEA KDKTGVFTGA 400
    YATNPVSGEQ VPVFIADYVL MGYGTGAIMA VPAHDTRDFA FARAFELSMR 450
    CVVEPSDDRG TDPSTWDDAF ASYDAKIVNS SSGEISLDGL GVVEAKARIT 500
    EWLERKGLGE GTVNFRLRDW LFSRQRYWGE PFPIVYDEDG IAHALPESML 550
    PLELPEVEDY SPRTFDPDDA NTSPETPLSR NEDWVNVTLD LGDGRGPQKY 600
    RRETNTMPNW AGSCWYELRY LDPHNDQKLV DPAIEQYWMG PREGQPTGGV 650
    DLYVGGAEHA VLHLLYARFW SKVLFDLGHI SSAEPFHKLF NQGMIQAYVY 700
    RDSRGIAVPA AEVEERDGAY YYQGEKVSRL LGKMGKSLKN AVTPDEICAE 750
    YGADTLRLYE MAMGPLDVSR PWDTRAVVGQ FRLLQRLWRN VVDEATGEVT 800
    VVDAEPDEET LRALHKAIDG VRQDLEGMRF NTAIAKVTEL NNQLTKAGGP 850
    VPRTVAESLV LLVAPLAPHI AEELWRKLGH NDSVVHQDFP VADPAYVVDE 900
    AVTCVVQIKG KVKARLEVSP AISEEELEKV ALADEKVVAA LGGAGIRKVI 950
    VRAPKLVNIV TA 962
    Length:962
    Mass (Da):106,570
    Last modified:June 1, 2003 - v1
    Checksum:i5EB57AE371334BF8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000030 Genomic DNA. Translation: BAC73200.1.
    RefSeqiNP_826665.1. NC_003155.4.

    Genome annotation databases

    EnsemblBacteriaiBAC73200; BAC73200; SAV_5488.
    GeneIDi1211296.
    KEGGisma:SAV_5488.
    PATRICi23724970. VBIStrAve112782_5842.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000030 Genomic DNA. Translation: BAC73200.1 .
    RefSeqi NP_826665.1. NC_003155.4.

    3D structure databases

    ProteinModelPortali Q82C66.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 227882.SAV_5488.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC73200 ; BAC73200 ; SAV_5488 .
    GeneIDi 1211296.
    KEGGi sma:SAV_5488.
    PATRICi 23724970. VBIStrAve112782_5842.

    Phylogenomic databases

    eggNOGi COG0495.
    HOGENOMi HOG000200748.
    KOi K01869.
    OMAi LGMFPYT.
    OrthoDBi EOG63Z74X.

    Enzyme and pathway databases

    BioCyci SAVE227882:GJU1-5564-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 4 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_00049_B. Leu_tRNA_synth_B.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites."
      Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M.
      Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.
    2. "Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis."
      Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S.
      Nat. Biotechnol. 21:526-531(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.

    Entry informationi

    Entry nameiSYL_STRAW
    AccessioniPrimary (citable) accession number: Q82C66
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2004
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3