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Q82C66 (SYL_STRAW) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:SAV_5488
OrganismStreptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680) [Complete proteome] [HAMAP]
Taxonomic identifier227882 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length962 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 962962Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152092

Regions

Motif68 – 7912"HIGH" region HAMAP-Rule MF_00049
Motif733 – 7375"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site7361ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q82C66 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 5EB57AE371334BF8

FASTA962106,570
        10         20         30         40         50         60 
MSETNPAAAS EVAAPHRYTA AVAAEIEARW QDFWDAEGTY EAPNPTGDLA GGPALVAKPK 

        70         80         90        100        110        120 
KFVMDMFPYP SGAGLHVGHP LGYIATDVFA RFQRMTGHNV LHTLGFDAFG LPAEQYAVQT 

       130        140        150        160        170        180 
GTHPRVSTEA NIENMKVQLR RLGLGHDKRR SFATIDPDYY KWTQWIFLQI FNSWYDEEAN 

       190        200        210        220        230        240 
KARPIAELVA QFESGERAVP RADGATRAWR ELTAAERADV LGEYRLAYAS EAPVNWSPGL 

       250        260        270        280        290        300 
GTVLANEEVT ADGRSERGNF PVFKAKLRQW NMRITAYADR LLDDLDALDW PEAIKLQQRN 

       310        320        330        340        350        360 
WIGRSEGARV DFPIDGEAVT VFTTRPDTLF GATYMVLAPE HPLVEKFTPA AWPEGTHDVW 

       370        380        390        400        410        420 
TGGHATPAEA VAAYRAQAAS KSDVERQAEA KDKTGVFTGA YATNPVSGEQ VPVFIADYVL 

       430        440        450        460        470        480 
MGYGTGAIMA VPAHDTRDFA FARAFELSMR CVVEPSDDRG TDPSTWDDAF ASYDAKIVNS 

       490        500        510        520        530        540 
SSGEISLDGL GVVEAKARIT EWLERKGLGE GTVNFRLRDW LFSRQRYWGE PFPIVYDEDG 

       550        560        570        580        590        600 
IAHALPESML PLELPEVEDY SPRTFDPDDA NTSPETPLSR NEDWVNVTLD LGDGRGPQKY 

       610        620        630        640        650        660 
RRETNTMPNW AGSCWYELRY LDPHNDQKLV DPAIEQYWMG PREGQPTGGV DLYVGGAEHA 

       670        680        690        700        710        720 
VLHLLYARFW SKVLFDLGHI SSAEPFHKLF NQGMIQAYVY RDSRGIAVPA AEVEERDGAY 

       730        740        750        760        770        780 
YYQGEKVSRL LGKMGKSLKN AVTPDEICAE YGADTLRLYE MAMGPLDVSR PWDTRAVVGQ 

       790        800        810        820        830        840 
FRLLQRLWRN VVDEATGEVT VVDAEPDEET LRALHKAIDG VRQDLEGMRF NTAIAKVTEL 

       850        860        870        880        890        900 
NNQLTKAGGP VPRTVAESLV LLVAPLAPHI AEELWRKLGH NDSVVHQDFP VADPAYVVDE 

       910        920        930        940        950        960 
AVTCVVQIKG KVKARLEVSP AISEEELEKV ALADEKVVAA LGGAGIRKVI VRAPKLVNIV 


TA 

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References

[1]"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites."
Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M.
Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.
[2]"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis."
Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S.
Nat. Biotechnol. 21:526-531(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000030 Genomic DNA. Translation: BAC73200.1.
RefSeqNP_826665.1. NC_003155.4.

3D structure databases

ProteinModelPortalQ82C66.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING227882.SAV_5488.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC73200; BAC73200; SAV_5488.
GeneID1211296.
KEGGsma:SAV_5488.
PATRIC23724970. VBIStrAve112782_5842.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycSAVE227882:GJU1-5564-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_STRAW
AccessionPrimary (citable) accession number: Q82C66
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 1, 2003
Last modified: April 16, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries