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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei736ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:SAV_5488
OrganismiStreptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
Taxonomic identifieri227882 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces
Proteomesi
  • UP000000428 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001520921 – 962Leucine--tRNA ligaseAdd BLAST962

Interactioni

Protein-protein interaction databases

STRINGi227882.SAV_5488.

Structurei

3D structure databases

ProteinModelPortaliQ82C66.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi68 – 79"HIGH" regionAdd BLAST12
Motifi733 – 737"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8T. Bacteria.
COG0495. LUCA.
HOGENOMiHOG000200748.
KOiK01869.
OMAiHRFIKRL.
OrthoDBiPOG091H01JZ.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 6 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q82C66-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSETNPAAAS EVAAPHRYTA AVAAEIEARW QDFWDAEGTY EAPNPTGDLA
60 70 80 90 100
GGPALVAKPK KFVMDMFPYP SGAGLHVGHP LGYIATDVFA RFQRMTGHNV
110 120 130 140 150
LHTLGFDAFG LPAEQYAVQT GTHPRVSTEA NIENMKVQLR RLGLGHDKRR
160 170 180 190 200
SFATIDPDYY KWTQWIFLQI FNSWYDEEAN KARPIAELVA QFESGERAVP
210 220 230 240 250
RADGATRAWR ELTAAERADV LGEYRLAYAS EAPVNWSPGL GTVLANEEVT
260 270 280 290 300
ADGRSERGNF PVFKAKLRQW NMRITAYADR LLDDLDALDW PEAIKLQQRN
310 320 330 340 350
WIGRSEGARV DFPIDGEAVT VFTTRPDTLF GATYMVLAPE HPLVEKFTPA
360 370 380 390 400
AWPEGTHDVW TGGHATPAEA VAAYRAQAAS KSDVERQAEA KDKTGVFTGA
410 420 430 440 450
YATNPVSGEQ VPVFIADYVL MGYGTGAIMA VPAHDTRDFA FARAFELSMR
460 470 480 490 500
CVVEPSDDRG TDPSTWDDAF ASYDAKIVNS SSGEISLDGL GVVEAKARIT
510 520 530 540 550
EWLERKGLGE GTVNFRLRDW LFSRQRYWGE PFPIVYDEDG IAHALPESML
560 570 580 590 600
PLELPEVEDY SPRTFDPDDA NTSPETPLSR NEDWVNVTLD LGDGRGPQKY
610 620 630 640 650
RRETNTMPNW AGSCWYELRY LDPHNDQKLV DPAIEQYWMG PREGQPTGGV
660 670 680 690 700
DLYVGGAEHA VLHLLYARFW SKVLFDLGHI SSAEPFHKLF NQGMIQAYVY
710 720 730 740 750
RDSRGIAVPA AEVEERDGAY YYQGEKVSRL LGKMGKSLKN AVTPDEICAE
760 770 780 790 800
YGADTLRLYE MAMGPLDVSR PWDTRAVVGQ FRLLQRLWRN VVDEATGEVT
810 820 830 840 850
VVDAEPDEET LRALHKAIDG VRQDLEGMRF NTAIAKVTEL NNQLTKAGGP
860 870 880 890 900
VPRTVAESLV LLVAPLAPHI AEELWRKLGH NDSVVHQDFP VADPAYVVDE
910 920 930 940 950
AVTCVVQIKG KVKARLEVSP AISEEELEKV ALADEKVVAA LGGAGIRKVI
960
VRAPKLVNIV TA
Length:962
Mass (Da):106,570
Last modified:June 1, 2003 - v1
Checksum:i5EB57AE371334BF8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000030 Genomic DNA. Translation: BAC73200.1.
RefSeqiWP_010986890.1. NZ_JZJK01000066.1.

Genome annotation databases

EnsemblBacteriaiBAC73200; BAC73200; SAVERM_5488.
KEGGisma:SAV_5488.
PATRICi23724970. VBIStrAve112782_5842.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000030 Genomic DNA. Translation: BAC73200.1.
RefSeqiWP_010986890.1. NZ_JZJK01000066.1.

3D structure databases

ProteinModelPortaliQ82C66.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi227882.SAV_5488.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC73200; BAC73200; SAVERM_5488.
KEGGisma:SAV_5488.
PATRICi23724970. VBIStrAve112782_5842.

Phylogenomic databases

eggNOGiENOG4105C8T. Bacteria.
COG0495. LUCA.
HOGENOMiHOG000200748.
KOiK01869.
OMAiHRFIKRL.
OrthoDBiPOG091H01JZ.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 6 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYL_STRAW
AccessioniPrimary (citable) accession number: Q82C66
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.