Q82AE4 (RSMH_STRAW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	SAV_6114

Organism

Streptomyces avermitilis [Complete proteome] [HAMAP]

Taxonomic identifier

33903 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP MF_01007
Subcellular location	Cytoplasm By similarity HAMAP MF_01007.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Ribosomal RNA small subunit methyltransferase H HAMAP MF_01007		PRO_0000108716
Regions
	Region	37 – 39	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	56	1	S-adenosyl-L-methionine By similarity
	Binding site	83	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	104	1	S-adenosyl-L-methionine By similarity
	Binding site	111	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q82AE4 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 081CD421891D8283	FASTA	318	34,704
	10 20 30 40 50 60 MSQSRHVPVM LQRCLDMLAP ALTEPGAVVV DCTLGLGGHS EALLTQFPEA RLVALDRDKE 70 80 90 100 110 120 ALRLSGERLA PFGDRATLVH AVYDELPDVL DRLGVPRVQG VLFDLGVSSM QLDEADRGFA 130 140 150 160 170 180 YAQDAPLDMR MDQTTGISAA EVLNTYPPGE IVRILRAYGE EKQAKRIVSA IVREREKEPF 190 200 210 220 230 240 SNSARLVELI RDSLPQAAKR TGGNPAKRTF QALRIEVNGE LSVLERAIPA AVKALAVGGR 250 260 270 280 290 300 IAVLSYHSLE DRLVKQVFAA GAATTAPPGL PVVPERYQPR LKLLTRGAEL PTEEEIAENR 310 RAAPARLRGA QRIREDAE

References

[1]

"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites." Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed: 11572948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

[2]

"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis." Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. Nat. Biotechnol. 21:526-531(2003) [PubMed: 12692562] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000030 Genomic DNA. Translation: BAC73825.1.
RefSeq	NP_827290.1. NC_003155.4.
3D structure databases
ProteinModelPortal	Q82AE4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1216552.
GenomeReviews	Gene locus SAV_6114 in contig BA000030_GR.
KEGG	sma:SAV_6114.
NMPDR	fig|227882.1.peg.6116.
PATRIC	23726260. VBIStrAve112782_6483.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG302779.
OMA	NPATRAF.
ProtClustDB	PRK00050.
Enzyme and pathway databases
BioCyc	SAVE227882:SAV6114-MONOMER.
Family and domain databases
HAMAP	MF_01007. 16SrRNA_methyltr_H. [Tree]
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
KO	K03438.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_STRAW

Accession

Primary (citable) accession number: Q82AE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program