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Q82AC9

- SYI_STRAW

UniProt

Q82AC9 - SYI_STRAW

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei603 – 6031ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSAVE227882:GJU1-6210-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:SAV_6130
OrganismiStreptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680)
Taxonomic identifieri227882 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces
ProteomesiUP000000428: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10471047Isoleucine--tRNA ligasePRO_0000098563Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi227882.SAV_6130.

Structurei

3D structure databases

ProteinModelPortaliQ82AC9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi52 – 6211"HIGH" regionAdd
BLAST
Motifi600 – 6045"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiPRFKTMK.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q82AC9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKPPQYRQVP AQVDLPALEH AVLDFWREQK IFAKSLEQSE GRPEWVFYEG
60 70 80 90 100
PPTANGMPGA HHIEARVFKD VFPRFRTMRG YHVARKAGWD CHGLPVELAV
110 120 130 140 150
EKELGFSGKQ DIEAYGIAEF NAKCRESVTR HTDAFEELTS RMGYWTDLND
160 170 180 190 200
PYRTMDPEYI ESVWWSLKEI FNKGLLVQDY RVAPWCPQDE TGLSDHELAQ
210 220 230 240 250
GYETIVDPSV YVRFPLTSGP LAGRAALLVW TTTPWTLVSN TAVAAHPDVT
260 270 280 290 300
YVVATNGEEK LVVAEPLVEK ALGEGWETTG ETFTGAEMER WTYQRPFELV
310 320 330 340 350
EFPAPAHYVV NAEYVTTEDG TGLVHQSPAF GEDDLKVCRE YGLPVVNPVR
360 370 380 390 400
TNGTFEEDVP LVGGVFFKKA DEKLTEDLQN RGLLFKHIPY EHSYPHCWRC
410 420 430 440 450
HTALLYYAQP SWYIRTTAVK DRLLQENEKT NWFPESVKHG RFGDWLNNNV
460 470 480 490 500
DWALSRSRFW GTPLPLWTCE EGHLTCVGSR AELSELTGTD QSNLDPHRPF
510 520 530 540 550
IDAVTFACPQ DGCGRTATRV PEVIDAWYDS GSMPFAQWGY PYKNKELFES
560 570 580 590 600
RYPAQFISEA IDQTRGWFYT LMAVGTLVFD KSSYENVVCL GHILAEDGRK
610 620 630 640 650
MSKHLGNILQ PIPLMDQHGA DAVRWFMAAG GSPWAARRVG HGTIQEVVRK
660 670 680 690 700
TLLTYWNTVA FQALYARTSG WAPSEADPAP ADRPVLDRWL LSELHALTDQ
710 720 730 740 750
VTQALESYDT QRAGKLLSAF VDDLSNWYVR RSRRRFWQGD KAALRTLHEV
760 770 780 790 800
VETVTRLMAP LTPFITERVW QDLVFPVTPG APESVHLASW PEADLSAIDP
810 820 830 840 850
ELSKQMVLVR RLVELGRATR AESGVKTRQP LSRALVAVAG FETLDRELHA
860 870 880 890 900
QITEELNVTS LAALSEVGGS LVDTTAKANF RALGKRFGKG VQAVAKAVAG
910 920 930 940 950
ADAAALSLAL REGTASVEVD GETVTLAPDE VIITETPREG WSVASDSGAT
960 970 980 990 1000
VALDLEITEE LRQAGLARDA IRLIQEARKN SGLDVADRIA LRWTSTDPEV
1010 1020 1030 1040
IAALSEHSEL IADEVLATDF AQGEADDTYG EPFTDESLSL TFRLRKA
Length:1,047
Mass (Da):117,130
Last modified:June 1, 2003 - v1
Checksum:iB08ACA994C0BA6BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000030 Genomic DNA. Translation: BAC73841.1.
RefSeqiNP_827306.1. NC_003155.4.
WP_010987531.1. NC_003155.4.

Genome annotation databases

EnsemblBacteriaiBAC73841; BAC73841; SAV_6130.
GeneIDi1211413.
KEGGisma:SAV_6130.
PATRICi23726294. VBIStrAve112782_6500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000030 Genomic DNA. Translation: BAC73841.1 .
RefSeqi NP_827306.1. NC_003155.4.
WP_010987531.1. NC_003155.4.

3D structure databases

ProteinModelPortali Q82AC9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 227882.SAV_6130.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC73841 ; BAC73841 ; SAV_6130 .
GeneIDi 1211413.
KEGGi sma:SAV_6130.
PATRICi 23726294. VBIStrAve112782_6500.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi PRFKTMK.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SAVE227882:GJU1-6210-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites."
    Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M.
    Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.
  2. "Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis."
    Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S.
    Nat. Biotechnol. 21:526-531(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.

Entry informationi

Entry nameiSYI_STRAW
AccessioniPrimary (citable) accession number: Q82AC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2003
Last modified: November 26, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3