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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei603ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSAVE227882:G1G23-6428-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:SAV_6130
OrganismiStreptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
Taxonomic identifieri227882 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces
Proteomesi
  • UP000000428 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000985631 – 1047Isoleucine--tRNA ligaseAdd BLAST1047

Proteomic databases

PRIDEiQ82AC9

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi227882.SAV_6130

Structurei

3D structure databases

ProteinModelPortaliQ82AC9
SMRiQ82AC9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi52 – 62"HIGH" regionAdd BLAST11
Motifi600 – 604"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246403
KOiK01870
OMAiPSWYIRT
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit

Sequencei

Sequence statusi: Complete.

Q82AC9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPPQYRQVP AQVDLPALEH AVLDFWREQK IFAKSLEQSE GRPEWVFYEG
60 70 80 90 100
PPTANGMPGA HHIEARVFKD VFPRFRTMRG YHVARKAGWD CHGLPVELAV
110 120 130 140 150
EKELGFSGKQ DIEAYGIAEF NAKCRESVTR HTDAFEELTS RMGYWTDLND
160 170 180 190 200
PYRTMDPEYI ESVWWSLKEI FNKGLLVQDY RVAPWCPQDE TGLSDHELAQ
210 220 230 240 250
GYETIVDPSV YVRFPLTSGP LAGRAALLVW TTTPWTLVSN TAVAAHPDVT
260 270 280 290 300
YVVATNGEEK LVVAEPLVEK ALGEGWETTG ETFTGAEMER WTYQRPFELV
310 320 330 340 350
EFPAPAHYVV NAEYVTTEDG TGLVHQSPAF GEDDLKVCRE YGLPVVNPVR
360 370 380 390 400
TNGTFEEDVP LVGGVFFKKA DEKLTEDLQN RGLLFKHIPY EHSYPHCWRC
410 420 430 440 450
HTALLYYAQP SWYIRTTAVK DRLLQENEKT NWFPESVKHG RFGDWLNNNV
460 470 480 490 500
DWALSRSRFW GTPLPLWTCE EGHLTCVGSR AELSELTGTD QSNLDPHRPF
510 520 530 540 550
IDAVTFACPQ DGCGRTATRV PEVIDAWYDS GSMPFAQWGY PYKNKELFES
560 570 580 590 600
RYPAQFISEA IDQTRGWFYT LMAVGTLVFD KSSYENVVCL GHILAEDGRK
610 620 630 640 650
MSKHLGNILQ PIPLMDQHGA DAVRWFMAAG GSPWAARRVG HGTIQEVVRK
660 670 680 690 700
TLLTYWNTVA FQALYARTSG WAPSEADPAP ADRPVLDRWL LSELHALTDQ
710 720 730 740 750
VTQALESYDT QRAGKLLSAF VDDLSNWYVR RSRRRFWQGD KAALRTLHEV
760 770 780 790 800
VETVTRLMAP LTPFITERVW QDLVFPVTPG APESVHLASW PEADLSAIDP
810 820 830 840 850
ELSKQMVLVR RLVELGRATR AESGVKTRQP LSRALVAVAG FETLDRELHA
860 870 880 890 900
QITEELNVTS LAALSEVGGS LVDTTAKANF RALGKRFGKG VQAVAKAVAG
910 920 930 940 950
ADAAALSLAL REGTASVEVD GETVTLAPDE VIITETPREG WSVASDSGAT
960 970 980 990 1000
VALDLEITEE LRQAGLARDA IRLIQEARKN SGLDVADRIA LRWTSTDPEV
1010 1020 1030 1040
IAALSEHSEL IADEVLATDF AQGEADDTYG EPFTDESLSL TFRLRKA
Length:1,047
Mass (Da):117,130
Last modified:June 1, 2003 - v1
Checksum:iB08ACA994C0BA6BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000030 Genomic DNA Translation: BAC73841.1
RefSeqiWP_010987531.1, NZ_JZJK01000089.1

Genome annotation databases

EnsemblBacteriaiBAC73841; BAC73841; SAVERM_6130
KEGGisma:SAVERM_6130

Similar proteinsi

Entry informationi

Entry nameiSYI_STRAW
AccessioniPrimary (citable) accession number: Q82AC9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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