Reviewed, UniProtKB/Swiss-Prot Q828M2 (SYC2_STRAW)

Names and origin

Protein names

Recommended name:
    Putative cysteinyl-tRNA synthetase 2
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase 2
      Short name=CysRS 2

Gene names

Name:	cysS2
Ordered Locus Names:	SAV_6647

Organism

Streptomyces avermitilis [Complete proteome] [HAMAP]

Taxonomic identifier

33903 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 409	409	Putative cysteinyl-tRNA synthetase 2 HAMAP MF_00041		PRO_0000159489
Regions
	Motif	286 – 290	5	"KMSKS" region HAMAP MF_00041
Sites
	Binding site	289	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q828M2-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 6D63CA8323100F67	FASTA	409	44,117
	10 20 30 40 50 60 MHAWPASEVP ALPGEGRDLR IHDTATGGVI PLHPGPVARI YVCGITPYDA THMGHAATYN 70 80 90 100 110 120 AFDLVQRVWL DTKRQVHYVQ NVTDVDDPLL ERAERDGVDW VGLAEKETAL FREDMTALRM 130 140 150 160 170 180 LPPQHYIGAV EAIPGIVPLV ERLRDAGAAY ELDGDVYFSV EADPHFGKVS NLDAAAMRLL 190 200 210 220 230 240 SAERGGDPDR PGKKNPLDPM LWMAAREGEP SWDGASLGRG RPGWHIECVA IALDHLGMGF 250 260 270 280 290 300 DVQGGGSDLA FPHHEMGASH AQALTGEFPM AKAYVHAGMV ALNGEKMSKS KGNLVFVSKL 310 320 330 340 350 360 RRDGVDPAAI RLALLAHHYR SDWEWTDAVL EEALARLGTW RAAVSRPDGP PAEALVEEIR 370 380 390 400 DALANDLDAP AALAAVDRWA ALQHERGGTD EGAPGVVSRA VDALLGVAL

References

[1]

"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites." Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed: 11572948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

[2]

"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis." Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. Nat. Biotechnol. 21:526-531(2003) [PubMed: 12692562] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000030 Genomic DNA. Translation: BAC74358.1.
RefSeq	NP_827823.1.
3D structure databases
SMR	Q828M2. Positions 20-397.
ModBase	Search...
Genome annotation databases
GeneID	1211523.
GenomeReviews	Gene locus SAV_6647 in contig BA000030_GR.
KEGG	sma:SAV_6647.
NMPDR	fig|227882.1.peg.6649.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG327651.
OMA	ALFREDM.
Enzyme and pathway databases
BioCyc	SAVE227882:SAV6647-MONOMER.
BRENDA	6.1.1.16. 140873.
Family and domain databases
HAMAP	MF_00041_B. Cys_tRNA_synth_B. Divergent sequence. [Tree]
InterPro	IPR015804. Cys-tRNA-synt_Ia_C. IPR015803. Cys-tRNA-synt_Ia_N. IPR017812. Mycothiol_biosynthesis_MshC. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR03447. mycothiol_MshC. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC2_STRAW

Accession

Primary (citable) accession number: Q828M2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)