Reviewed, UniProtKB/Swiss-Prot Q828K5 (GLPK1_STRAW)

Names and origin

Protein names

Recommended name:
    Glycerol kinase 1
    EC=2.7.1.30
Alternative name(s):
    ATP:glycerol 3-phosphotransferase 1
    Glycerokinase 1
      Short name=GK 1

Gene names

Name:	glpK1
Ordered Locus Names:	SAV_6664

Organism

Streptomyces avermitilis [Complete proteome] [HAMAP]

Taxonomic identifier

33903 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glycerol kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 512	512	Glycerol kinase 1 HAMAP MF_00186		PRO_0000059499
Regions
	Nucleotide binding	422 – 426	5	ATP By similarity
Sites
	Binding site	18	1	Substrate By similarity
	Binding site	22	1	ATP By similarity
	Binding site	88	1	Substrate By similarity
	Binding site	140	1	Substrate By similarity
	Binding site	255	1	Substrate By similarity
	Binding site	277	1	ATP By similarity
	Binding site	321	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q828K5-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 5ABBFA23151391A2	FASTA	512	55,846
	10 20 30 40 50 60 MTDAHTAGPF IAAIDQGTTS SRCIVFDRDG RIVSVDQKEH EQIFPKPGWV EHNAAEIWTN 70 80 90 100 110 120 VQEVVAGAVE KAGITRDDIK AIGITNQRET TLLWDKNTGE PVHNALVWQD TRTDALCKEL 130 140 150 160 170 180 GRNVGQDRFR RETGLPLASY FAGPKARWLL DNVEGLRERA EAGDILFGTM DTWVIWNLTG 190 200 210 220 230 240 GVNGGKHVTD VTNASRTMLM NLHTMQWDDK IAESIGVPLA MLPEIRSSAE VYGEITGGKL 250 260 270 280 290 300 GDLLGGIPVA SALGDQQAAL FGQTCFSEGE AKSTYGTGTF MLMNTGDKII NSYSGLLTTV 310 320 330 340 350 360 GYQIGDQKPV YALEGSIAVT GSLVQWMRDQ MGLIKSAAEI ETLASSVEDN GGAYFVPAFS 370 380 390 400 410 420 GLFAPYWRSD ARGVIAGLTR YVTKAHIARA VLEATAWQTR EITDAMTKDS GVELAALKVD 430 440 450 460 470 480 GGMTSNNLLM QTLSDFLDAP VVRPMVAETT CLGAAYAAGL AVGFWTNTED LRANWRRAAE 490 500 510 WTPNMAAETR DREYKSWLKA VERTMGWIED EE

References

[1]

"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites." Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed: 11572948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

[2]

"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis." Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. Nat. Biotechnol. 21:526-531(2003) [PubMed: 12692562] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000030 Genomic DNA. Translation: BAC74375.1.
RefSeq	NP_827840.1.
3D structure databases
SMR	Q828K5. Positions 10-511.
ModBase	Search...
Genome annotation databases
GeneID	1211529.
GenomeReviews	Gene locus SAV_6664 in contig BA000030_GR.
KEGG	sma:SAV_6664.
NMPDR	fig|227882.1.peg.6666.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG511469.
OMA	LMERYEP.
Enzyme and pathway databases
BioCyc	SAVE227882:SAV6664-MONOMER.
BRENDA	2.7.1.30. 140873.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. False negative. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK1_STRAW

Accession

Primary (citable) accession number: Q828K5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)