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Q828I8

- PSB2_STRAW

UniProt

Q828I8 - PSB2_STRAW

Protein

Proteasome subunit beta 2

Gene

prcB2

Organism
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.UniRule annotation

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.UniRule annotation

    Enzyme regulationi

    The formation of the proteasomal ATPase ARC-20S proteasome complex, likely via the docking of the C-termini of ARC into the intersubunit pockets in the alpha-rings, may trigger opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei54 – 541NucleophileUniRule annotation

    GO - Molecular functioni

    1. threonine-type endopeptidase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. modification-dependent protein catabolic process Source: UniProtKB-HAMAP
    2. proteasomal protein catabolic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Enzyme and pathway databases

    BioCyciSAVE227882:GJU1-6767-MONOMER.
    UniPathwayiUPA00997.

    Protein family/group databases

    MEROPSiT01.005.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit beta 2UniRule annotation (EC:3.4.25.1UniRule annotation)
    Alternative name(s):
    20S proteasome beta subunit 2UniRule annotation
    Proteasome core protein PrcB 2UniRule annotation
    Gene namesi
    Name:prcB2UniRule annotation
    Synonyms:prcB1
    Ordered Locus Names:SAV_6681
    OrganismiStreptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680)
    Taxonomic identifieri227882 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces
    ProteomesiUP000000428: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. proteasome core complex, beta-subunit complex Source: UniProtKB-HAMAP

    Keywords - Cellular componenti

    Cytoplasm, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 5353Removed in mature form; by autocatalysisUniRule annotationPRO_0000397584Add
    BLAST
    Chaini54 – 281228Proteasome subunit beta 2PRO_0000397585Add
    BLAST

    Keywords - PTMi

    Autocatalytic cleavage, Zymogen

    Interactioni

    Subunit structurei

    The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped by the proteasome-associated ATPase, ARC.UniRule annotation

    Protein-protein interaction databases

    STRINGi227882.SAV_6681.

    Structurei

    3D structure databases

    ProteinModelPortaliQ828I8.
    SMRiQ828I8. Positions 54-272.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1B family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0638.
    HOGENOMiHOG000245308.
    KOiK03433.
    OMAiFQVELEH.
    OrthoDBiEOG6XM79W.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    HAMAPiMF_02113_B. Proteasome_B_B.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR022483. Pept_T1A_Psome_suB_actinobac.
    IPR000243. Pept_T1A_subB.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    [Graphical view]
    PRINTSiPR00141. PROTEASOME.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR03690. 20S_bact_beta. 1 hit.
    PROSITEiPS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q828I8-1 [UniParc]FASTAAdd to Basket

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    MEANTRSTGR LPAAFLTPGS SSFMDFLSEH QPEILPGNRQ LPPTQGVIEA    50
    PHGTTIVATT FPGGVVLAGD RRATMGNVIA QRDIEKVFPA DEYSAVGIAG 100
    TAGLAVEMVK LFQLELEHFE KVEGATLSLE GKANRLSTMI RSNLAMAMQG 150
    LAVVPLFAGY DVDREKGRIF SYDVTGGRSE EHGYASTGSG SIFARGAMKK 200
    LYRDDLTEQQ ATTLVIQALY DAADDDSATG GPDVARRIYP IVTVITEDGF 250
    RRLTDEESSE IARAILERRL EQPDGPRAAL L 281
    Length:281
    Mass (Da):30,292
    Last modified:June 1, 2003 - v1
    Checksum:i9503B362CB551433
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000030 Genomic DNA. Translation: BAC74392.1.
    RefSeqiNP_827857.1. NC_003155.4.
    WP_010988081.1. NC_003155.4.

    Genome annotation databases

    EnsemblBacteriaiBAC74392; BAC74392; SAV_6681.
    GeneIDi1211534.
    KEGGisma:SAV_6681.
    PATRICi23727472. VBIStrAve112782_7084.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000030 Genomic DNA. Translation: BAC74392.1 .
    RefSeqi NP_827857.1. NC_003155.4.
    WP_010988081.1. NC_003155.4.

    3D structure databases

    ProteinModelPortali Q828I8.
    SMRi Q828I8. Positions 54-272.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 227882.SAV_6681.

    Protein family/group databases

    MEROPSi T01.005.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC74392 ; BAC74392 ; SAV_6681 .
    GeneIDi 1211534.
    KEGGi sma:SAV_6681.
    PATRICi 23727472. VBIStrAve112782_7084.

    Phylogenomic databases

    eggNOGi COG0638.
    HOGENOMi HOG000245308.
    KOi K03433.
    OMAi FQVELEH.
    OrthoDBi EOG6XM79W.

    Enzyme and pathway databases

    UniPathwayi UPA00997 .
    BioCyci SAVE227882:GJU1-6767-MONOMER.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    HAMAPi MF_02113_B. Proteasome_B_B.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR022483. Pept_T1A_Psome_suB_actinobac.
    IPR000243. Pept_T1A_subB.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    [Graphical view ]
    PRINTSi PR00141. PROTEASOME.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR03690. 20S_bact_beta. 1 hit.
    PROSITEi PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites."
      Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M.
      Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.
    2. "Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis."
      Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S.
      Nat. Biotechnol. 21:526-531(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.

    Entry informationi

    Entry nameiPSB2_STRAW
    AccessioniPrimary (citable) accession number: Q828I8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 10, 2010
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3