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Protein

Protein Tat

Gene

tat

Organism
Jembrana disease virus (JDV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication. Acts as a sequence-specific molecular adapter, directing components of the cellular transcription machinery to the viral RNA to promote processive transcription elongation by the RNA polymerase II (RNA pol II) complex, thereby increasing the level of full-length transcripts. Tat binds to a hairpin structure at the 5'-end of all nascent viral mRNAs referred to as the transactivation responsive RNA element (TAR RNA) in a CCNT1-independent mode. Tat then recruits the CCNT1/cyclin-T1 component of the P-TEFb complex (CDK9 and CCNT1), which promotes RNA chain elongation. The CDK9 component of P-TEFb hyperphosphorylates the C-terminus of RNA Pol II that becomes stabilized and much more processive (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Host-virus interaction, Transcription, Transcription regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Tat
Alternative name(s):
Transactivating regulatory protein
jTat
Gene namesi
Name:tat
OrganismiJembrana disease virus (JDV)
Taxonomic identifieri36370 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeLentivirusBovine lentivirus group
Virus hostiBos javanicus (Wild banteng) [TaxID: 9906]

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 114114Protein TatPRO_0000272361Add
BLAST

Interactioni

Subunit structurei

Binds to bovine CCNT1/cyclin-T1.By similarity

Structurei

Secondary structure

1
114
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi72 – 765Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZBNNMR-B65-81[»]
ProteinModelPortaliQ82854.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ82854.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni38 – 5316Cysteine-richBy similarityAdd
BLAST
Regioni54 – 6512CoreBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi66 – 8318Nuclear localization signal and RNA-binding (TAR)By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the lentiviruses Tat family.Curated

Family and domain databases

Gene3Di4.10.20.10. 1 hit.
InterProiIPR001831. IV_Tat.
[Graphical view]
PfamiPF00539. Tat. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q82854-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPGPWATTLT FPGHNGGFGG GPKCWLFWNT CAGPRRVCPK CSCPICVWHC
60 70 80 90 100
QLCFLQKGLG IRHDGRRKKR GTRGKGRKIH YARSITESGG QRAPNCASSS
110
ASCQTWALKH GINC
Length:114
Mass (Da):12,457
Last modified:November 1, 1996 - v1
Checksum:i77A7CB6FAF128D5A
GO
Isoform Short (identifier: Q82854-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     98-114: Missing.

Show »
Length:97
Mass (Da):10,682
Checksum:i4E5AAAB97D3FD8C2
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei98 – 11417Missing in isoform Short. CuratedVSP_022398Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21603 Genomic RNA. Translation: AAA64392.1.
U21603 Genomic RNA. Translation: AAA64395.1.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21603 Genomic RNA. Translation: AAA64392.1.
U21603 Genomic RNA. Translation: AAA64395.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZBNNMR-B65-81[»]
ProteinModelPortaliQ82854.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ82854.

Family and domain databases

Gene3Di4.10.20.10. 1 hit.
InterProiIPR001831. IV_Tat.
[Graphical view]
PfamiPF00539. Tat. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence analysis of Jembrana disease virus: a bovine lentivirus associated with an acute disease syndrome."
    Chadwick B.J., Coelen R.J., Wilcox G.E., Sammels L.M., Kertayadnya G.
    J. Gen. Virol. 76:1637-1650(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] (ISOFORMS LONG AND SHORT).
    Strain: Tabanan/87.
  2. "Characterization of the Jembrana disease virus tat gene and the cis- and trans-regulatory elements in its long terminal repeats."
    Chen H., Wilcox G., Kertayadnya G., Wood C.
    J. Virol. 73:658-666(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  3. "Jembrana disease virus Tat can regulate human immunodeficiency virus (HIV) long terminal repeat-directed gene expression and can substitute for HIV Tat in viral replication."
    Chen H., He J., Fong S., Wilcox G., Wood C.
    J. Virol. 74:2703-2713(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: BINDING TO HIV TAR RNA.
  4. Cited for: BINDING TO HIV AND BIV TAR RNA.
  5. "A single intermolecular contact mediates intramolecular stabilization of both RNA and protein."
    Calabro V., Daugherty M.D., Frankel A.D.
    Proc. Natl. Acad. Sci. U.S.A. 102:6849-6854(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 65-81 IN COMPLEX WITH BIV TAR RNA.

Entry informationi

Entry nameiTAT_JEMBR
AccessioniPrimary (citable) accession number: Q82854
Secondary accession number(s): Q82853
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: November 1, 1996
Last modified: October 14, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds also to BIV and HIV TAR RNAs.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.