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Protein

Argininosuccinate synthase

Gene

argG

Organism
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG), Argininosuccinate synthase (argG1)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei94CitrullineUniRule annotation1
Binding sitei124ATP; via amide nitrogenUniRule annotation1
Binding sitei126AspartateUniRule annotation1
Binding sitei130AspartateUniRule annotation1
Binding sitei130CitrullineUniRule annotation1
Binding sitei131AspartateUniRule annotation1
Binding sitei134CitrullineUniRule annotation1
Binding sitei182CitrullineUniRule annotation1
Binding sitei266CitrullineUniRule annotation1
Binding sitei278CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 23ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Synonyms:argG2
Ordered Locus Names:SAV_6778
OrganismiStreptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
Taxonomic identifieri227882 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces
Proteomesi
  • UP000000428 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001486451 – 404Argininosuccinate synthaseAdd BLAST404

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi227882.SAV_6778.

Structurei

3D structure databases

ProteinModelPortaliQ827Z1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CDH. Bacteria.
COG0137. LUCA.
HOGENOMiHOG000230093.
KOiK01940.
OMAiQCEVVTF.
OrthoDBiPOG091H01U0.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q827Z1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQCEEKPVTE RVVLAYSGGL DTSVAIGWIA EETGAEVIAV AVDVGQGGED
60 70 80 90 100
LDVIRKRALA CGAVEAEVAD AKDEFADEYC LPAVKANALY MDRYPLVSAL
110 120 130 140 150
SRPTIVKHLV AAAQKHGATT VAHGCTGKGN DQVRFEAGIV ALAPGLKCIA
160 170 180 190 200
PVRDYAMTRD KAIAFCEEKR LPIATTKKSP YSIDQNVFGR AVETGFLEDI
210 220 230 240 250
WNAPIEDIYE YTSNPAEPRE ADEVVISFKE GVPVAIDGRP VTVLQAIQQL
260 270 280 290 300
NERAGAQGVG RIDMVEDRLV GIKSREVYEA PGAIALITAH QELENVTVER
310 320 330 340 350
ELARYKRQVE QRWGELVYDG QWFSPLKRAL EGFIDEANQH VNGDIRMTLH
360 370 380 390 400
GGRAVVTGRR SETSLYDFNL ATYDTGDSFD QAAAKGFIDI YSLSSKIAAK

RDLA
Length:404
Mass (Da):44,066
Last modified:June 1, 2003 - v1
Checksum:i16FFAC320B810D8B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000030 Genomic DNA. Translation: BAC74489.1.

Genome annotation databases

EnsemblBacteriaiBAC74489; BAC74489; SAVERM_6778.
KEGGisma:SAV_6778.
PATRICi23727670. VBIStrAve112782_7184.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000030 Genomic DNA. Translation: BAC74489.1.

3D structure databases

ProteinModelPortaliQ827Z1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi227882.SAV_6778.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC74489; BAC74489; SAVERM_6778.
KEGGisma:SAV_6778.
PATRICi23727670. VBIStrAve112782_7184.

Phylogenomic databases

eggNOGiENOG4105CDH. Bacteria.
COG0137. LUCA.
HOGENOMiHOG000230093.
KOiK01940.
OMAiQCEVVTF.
OrthoDBiPOG091H01U0.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASSY_STRAW
AccessioniPrimary (citable) accession number: Q827Z1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.