Reviewed, UniProtKB/Swiss-Prot Q827H4 (MSOX_STRAW)

Names and origin

Protein names

Recommended name:
    Monomeric sarcosine oxidase
      Short name=MSOX
    EC=1.5.3.1

Gene names

Name:	soxA
Synonyms:	solA
Ordered Locus Names:	SAV_6950

Organism

Streptomyces avermitilis [Complete proteome] [HAMAP]

Taxonomic identifier

33903 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	384 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative demethylation of sarcosine By similarity.
Catalytic activity	Sarcosine + H2O + O2 = glycine + formaldehyde + H2O2. HAMAP MF_00516
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MSOX/MTOX family. MSOX subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sarcosine oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 384	384	Monomeric sarcosine oxidase HAMAP MF_00516		PRO_0000213763
Regions
	Nucleotide binding	6 – 36	31	FAD Potential
Amino acid modifications
	Modified residue	315	1	S-8alpha-FAD cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q827H4-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 28302788BB0DE585	FASTA	384	42,167
	10 20 30 40 50 60 MSPTYDVIVI GLGGMGSAAA HHLSARGARV LGLEKFGPVH NRGSSHGGSR ITRQSYFEDP 70 80 90 100 110 120 AYVPLLLRSY ELYEEVERST GREVATLSGG VMVGRPDSLT VAGSLRSATQ WDLPHEMLDA 130 140 150 160 170 180 KEIRRRFPTL NPSNDEVALY EKKAGLVRPE NMVAAHLQLA TRQGAELHFE EPMTRWEPYR 190 200 210 220 230 240 DGVRVHTAEN TYTAGQLVIC PGAWAPQLLT DLGVPFTIER QVMYWFQPRH GVGPFRPENH 250 260 270 280 290 300 PIYIWEDAEG VQVYGFPSID GPDLGAKVAF FRKGVVCTPE TIDRTVHDHE VQAMADHMSR 310 320 330 340 350 360 CIPDLPGTFL KAATCMYSNT PDEHFVIARH PAHPDSVTVA CGFSGHGFKF VPVVGEIVAD 370 380 LALTGTTAHP IGLFDPRRLA AAPA

References

[1]

"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites." Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed: 11572948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

[2]

"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis." Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. Nat. Biotechnol. 21:526-531(2003) [PubMed: 12692562] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000030 Genomic DNA. Translation: BAC74661.1.
RefSeq	NP_828126.1.
3D structure databases
HSSP	HSSP built from PDB template 1EL5 based on UniProtKB P40859.
ModBase	Search...
Genome annotation databases
GeneID	1211626.
GenomeReviews	Gene locus SAV_6950 in contig BA000030_GR.
NMPDR	fig|227882.1.peg.6952.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q827H4.
OMA	Q827H4. RIIRQAY.
Enzyme and pathway databases
BioCyc	SAVE227882:SAV6950-MON.
BRENDA	1.5.3.1. 140873.
Family and domain databases
HAMAP	MF_00516. [Tree]
InterPro	IPR006076. FAD-dep_OxRdtase. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MSOX_STRAW

Accession

Primary (citable) accession number: Q827H4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 4, 2003
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)