Q82707 (L_IHNVO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Including the following 3 domains:

1. RNA-directed RNA polymerase
   EC=2.7.7.48
2. mRNA (guanine-N(7)-)-methyltransferase
   EC=2.1.1.56
3. mRNA guanylyltransferase
   EC=2.7.7.-

Gene names

Name:

L

Organism

Infectious hematopoietic necrosis virus (strain Oregon69) (IHNV) [Complete proteome]

Taxonomic identifier

429315 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Novirhabdovirus

Virus host

Salmo [TaxID: 8028]

Protein attributes

Sequence length	1986 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently six subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subcellular location	Virion Potential. Host cytoplasm By similarity.
Sequence similarities	Belongs to the rhabdoviridae protein L family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Host cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	RNA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1986	1986	RNA-directed RNA polymerase L		PRO_0000282897
Regions
	Domain	560 – 757	198	RdRp catalytic
	Nucleotide binding	1619 – 1628	10	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q82707 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 25FE029E810846F9	FASTA	1,986	225,021
	10 20 30 40 50 60 MDFFDLDIEI KQERLPAECS LNSPLNYSLS AQLTDRMTPR TENVRRQRER LRSHMREHFR 70 80 90 100 110 120 VKDLSTLDND STRLHARLTE DLITIQSPEI DSSVLENWTP LKSYYASLDY TLPEKTAFKW 130 140 150 160 170 180 EHAAPYWNLF AQLRAILLQS QKIKKQETGT RELYSCGPLQ IEFVEGVVLY FTDRGSKEEF 190 200 210 220 230 240 TKSGELPSVT PYADFLAWIK IISQRAQAVL MAVILRVTDK GLSPLPESLL AIYQNVDDIL 250 260 270 280 290 300 KRAGQPAIDL LKLWEPLVIT KLGELLGDRF GLEEDFRNTI RGEATKLAKN LFISRGLNRL 310 320 330 340 350 360 MDILDQQTDA QPLFQFFGLF KHFAYPRVFS RDTIQAIQEV SDRPSSISAV DFLHDQCEIR 370 380 390 400 410 420 KEFYVRYINA YHRAPGLDLS ALSPSSFLRD SLERGKIPNE RSPLYSNKEW YFVKFTKSIE 430 440 450 460 470 480 WPVSDTLSTF LSDKAITRDR PAWIEDGHSG RDMSEKRLLL KFIKENFSSV ADIVGAAEAI 490 500 510 520 530 540 YNNQEDRLIA LKVKEMELKI KGRGFGLMTF MPRLLQVLRE SIAKKTQKLF PEITMTSSDL 550 560 570 580 590 600 DMKKRKFMLS KKSDDRRGFI HVNKSLDINK FCTSQRQFNS NAVFSSLDEL MGTFPLFSRV 610 620 630 640 650 660 HEIFEKTWIV DGSSSDPPNL AHFTRILDEC TALGLDTPHI WADGVFSGLK GGIEGLCQYV 670 680 690 700 710 720 WTICLLLRVE RVMQKTALTH YILAQGDNVI ITIIVPVEIH RDGTIPEQES RRILALSRDI 730 740 750 760 770 780 DLSLESELEK SGLTLKIEET LTSENISIYG KDLHCPQHLT LAIKKAASAA IISSEQYQDV 790 800 810 820 830 840 PTFLSGLGTS LEALSECVNN KVGVHLFGVI MGVAGWRDLA THQTWRGWRY PYHKKAITGR 850 860 870 880 890 900 IRASEMKLSK GEPTELSISV LSKRRRETET LIELLSNSLL GSALGMLAFP TPLDLEKRGV 910 920 930 940 950 960 GDYITHRLTI ARKALLSGHL DPRIGRKVES ACNIPLSSRT DLSKLFDSPF SLNIATEEDA 970 980 990 1000 1010 1020 TAVIKRQATK ILRLQEIKNE KLKAQIDNMD KGIATLDAAL AGATNINPRL NYMIRSITDE 1030 1040 1050 1060 1070 1080 KESEMFVTKF ASARTMRTLA MNHSSELPIV ILLEMKSQQK ETYTIWRTKR PPVTMWKCST 1090 1100 1110 1120 1130 1140 VLAKELRDTS WGKNIIGGTS PSPIEAMETI QIDPTEWEDR RSQDAMSINY YLSRAGMDEQ 1150 1160 1170 1180 1190 1200 TAKLTRGFLV PYYGTQTKPL VAKAYLELKG NPRTNKALLL LSVRESLVKT GSNLDKLIIK 1210 1220 1230 1240 1250 1260 LCSHALDIDV ASLPALRAQE EAAAGEGLRG GIKESMSPVG PDNFYTNITH KVFNRKWATP 1270 1280 1290 1300 1310 1320 YHVNIADFII QGLIETRRHL LVNERMNGLL PVSSVKCTSC FRKKEREFFD IPEEFTWKNE 1330 1340 1350 1360 1370 1380 SKTSDPAYTY FTTWCDLPRV SNLPEMDQRS ATRLLGRGLA LNRSSSGEII TKFYSMPMES 1390 1400 1410 1420 1430 1440 QRLLHPVELL LGYGEGVIFG YLRSQHINHG ALFHIGDESL AKKLRRYVLD TKTQHAKQIG 1450 1460 1470 1480 1490 1500 YLFQDEDSLH ELLGQGLCPY IPRSIPLTIT ELTNACAITT IRATEVILST KARIHHMPVQ 1510 1520 1530 1540 1550 1560 AIDESDVDTS RLAANTMQTI LGDPRPMNLV HLDCDLTHNM VAWESEVELD ILKSENFHID 1570 1580 1590 1600 1610 1620 GLLVELTARE LPIGDTPWKQ RDWTCSNDPK IIAKGIKTKS LFIHQGVTGA INLIPDLLVV 1630 1640 1650 1660 1670 1680 IGGGLGGCAV PYLQEWPDTP IIFATLFDER ERISEDGDLI VPPELLVRGM APRMIEREIL 1690 1700 1710 1720 1730 1740 EAELCDITNE GNRRLLIRLV TKNKGGGKVV LIDEIENRGA PESLLQSSLQ DLFEKLDKVC 1750 1760 1770 1780 1790 1800 KLTSVHTVRE STVEQFSQRV NSIKRSRKAV TLHWNRYNRR DQFEALVIVK GEEAKSDYRI 1810 1820 1830 1840 1850 1860 STITSAKAFR KIDEQLEIDG RLSSTQWSLP ALPSREKNIL FGYVSSVFLK MNLALSANDM 1870 1880 1890 1900 1910 1920 DRERLIETIE GTAPGLISWK EKLEHRDHAH RSDIEEKGIT QDKIFNLICL SWVLKGLRYG 1930 1940 1950 1960 1970 1980 VWDTDAQSIV AQTVYITRGP KLCPLGEKPK RIFASFKLQS GKRVEDAKGF LSALLHLEGF FPLGEQ

References

[1]	"Identification of the non-virion (NV) protein of fish rhabdoviruses viral haemorrhagic septicaemia virus and infectious haematopoietic necrosis virus." Schutze H., Enzmann P.J., Mundt E., Mettenleiter T.C. J. Gen. Virol. 77:1259-1263(1996) [PubMed: 8683214] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Complete genomic sequence of the fish rhabdovirus infectious haematopoietic necrosis virus." Schutze H., Enzmann P.J., Kuchling R., Mundt E., Niemann H., Mettenleiter T.C. J. Gen. Virol. 76:2519-2527(1995) [PubMed: 7595355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X89213 Genomic RNA. Translation: CAA61500.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR014023. RNA-dir_pol_cat. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_IHNVO

Accession

Primary (citable) accession number: Q82707

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program