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Protein

Probable DNA ligase

Gene

lig

Organism
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei208 – 2081ATPUniRule annotation
Active sitei210 – 2101N6-AMP-lysine intermediateUniRule annotation
Binding sitei215 – 2151ATPUniRule annotation
Binding sitei230 – 2301ATPUniRule annotation
Binding sitei259 – 2591ATPUniRule annotation
Binding sitei299 – 2991ATPUniRule annotation
Binding sitei374 – 3741ATPUniRule annotation
Binding sitei380 – 3801ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAVE227882:GJU1-7232-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]UniRule annotation
Gene namesi
Name:ligUniRule annotation
Ordered Locus Names:SAV_7136
OrganismiStreptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680)
Taxonomic identifieri227882 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces
ProteomesiUP000000428 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 512512Probable DNA ligasePRO_0000365240Add
BLAST

Proteomic databases

PRIDEiQ826P6.

Interactioni

Protein-protein interaction databases

STRINGi227882.SAV_7136.

Structurei

3D structure databases

ProteinModelPortaliQ826P6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1793.
HOGENOMiHOG000036008.
KOiK10747.
OMAiGRPRPFQ.
OrthoDBiEOG6Q8HXT.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 2 hits.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q826P6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLSRLAHVS REVAATSARS RKIALLAELF RDADADDVPI VIPYLAGRLP
60 70 80 90 100
QGRLGIGWKL LGRPVTPASE PSLTVRDVDA RLTAVGAVTG TGSQAGRGRL
110 120 130 140 150
MGELMAAATD VEQAFLRGLL TGEVRQGALD AVAVEGLAEA TGAPPADVRR
160 170 180 190 200
AVMLAGSLQT VARALLAEGP GALDGFRLTV GRPVLPMLAH TAGSVAEAVG
210 220 230 240 250
KLGACAVEEK LDGIRVQLHR DGDHVRIYTR TLDDITDRLP ELTAAALELK
260 270 280 290 300
GDRFILDGEV IALDGNGRPR SFQEIAGRVG SRVDVAAAAA SVPVSPVFFD
310 320 330 340 350
ALSVDGRDLL DLPFTERHAE LARLVPEPLR VRRALVSGPD DGAAAETFLA
360 370 380 390 400
DTLARGHEGV VVKALDAPYS AGRRGASWLK VKPVHTLDLV VLAAEWGHGR
410 420 430 440 450
RTGKLSNLHL GARAADGSFA MLGKTFKGMT DAMLTWQTER LTELAVEENG
460 470 480 490 500
SVVTVRPELV VEIAYDGLQK STRYPAGVTL RFARVVRYRE DKTPAEADTV
510
ETLLAAHPEV TR
Length:512
Mass (Da):54,366
Last modified:June 1, 2003 - v1
Checksum:i83BCF5CCA086779F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000030 Genomic DNA. Translation: BAC74847.1.
RefSeqiNP_828312.1. NC_003155.4.
WP_010988531.1. NZ_BAVY01000018.1.

Genome annotation databases

EnsemblBacteriaiBAC74847; BAC74847; SAV_7136.
KEGGisma:SAV_7136.
PATRICi23728432. VBIStrAve112782_7555.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000030 Genomic DNA. Translation: BAC74847.1.
RefSeqiNP_828312.1. NC_003155.4.
WP_010988531.1. NZ_BAVY01000018.1.

3D structure databases

ProteinModelPortaliQ826P6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi227882.SAV_7136.

Proteomic databases

PRIDEiQ826P6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC74847; BAC74847; SAV_7136.
KEGGisma:SAV_7136.
PATRICi23728432. VBIStrAve112782_7555.

Phylogenomic databases

eggNOGiCOG1793.
HOGENOMiHOG000036008.
KOiK10747.
OMAiGRPRPFQ.
OrthoDBiEOG6Q8HXT.

Enzyme and pathway databases

BioCyciSAVE227882:GJU1-7232-MONOMER.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 2 hits.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites."
    Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M.
    Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.
  2. "Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis."
    Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S.
    Nat. Biotechnol. 21:526-531(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.

Entry informationi

Entry nameiDNLI_STRAW
AccessioniPrimary (citable) accession number: Q826P6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: June 1, 2003
Last modified: May 27, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.