Reviewed, UniProtKB/Swiss-Prot Q826J2 (GLPK3_STRAW)

Names and origin

Protein names

Recommended name:
    Glycerol kinase 3
    EC=2.7.1.30
Alternative name(s):
    ATP:glycerol 3-phosphotransferase 3
    Glycerokinase 3
      Short name=GK 3

Gene names

Name:	glpK3
Ordered Locus Names:	SAV_7201

Organism

Streptomyces avermitilis [Complete proteome] [HAMAP]

Taxonomic identifier

33903 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glycerol kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 505	505	Glycerol kinase 3 HAMAP MF_00186		PRO_0000059501
Regions
	Nucleotide binding	416 – 420	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	249	1	Substrate By similarity
	Binding site	271	1	ATP By similarity
	Binding site	315	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q826J2-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2AD9A335522CA323	FASTA	505	55,291
	10 20 30 40 50 60 MADFVGAVDQ GTTSTRFMIF DHAGNEVAKH QLEHAQILPR SGWVEHDPVE IWERTNSVIQ 70 80 90 100 110 120 NALRHGNLSP DDLAAIGITN QRETTVVWDP RNGRPYYNAI VWQDTRTDSI AAALERSGQG 130 140 150 160 170 180 DVIRRKAGLP PATYFSGGKI QWILENVDGV REAAEQGHAV FGNTDCWVLW NLTGGPDGGI 190 200 210 220 230 240 HATDVTNASR TMLMDLETLD WDDELLGLFD IPRSMLPTIN PSSHPEAYGQ TRTSRPLRAA 250 260 270 280 290 300 IPIAGVLGDQ QAATVGQVCY APGEAKNTYG TGNFLVLNTG TELVRSQHGL LTTVAYQFGD 310 320 330 340 350 360 SPAIYALEGS IAVTGSAVQW LRDQMKIIKS AAESETLART VEDNGGIYFV PAFSGLFAPY 370 380 390 400 410 420 WRSDARGAIV GLARYHDNGH LARATLEAIC YQSRDVVEAM EQDSGVHLDV LKVDGGVTAN 430 440 450 460 470 480 DLCMQIQADI LGVPVSRPVV AETTALGAAY AAGLATGFWQ DTDELRTHWQ ESKRWEPQWS 490 500 EDRRAEGYAG WKKAVERTLD WSKVE

References

[1]

"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites." Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed: 11572948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

[2]

"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis." Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. Nat. Biotechnol. 21:526-531(2003) [PubMed: 12692562] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000030 Genomic DNA. Translation: BAC74912.1.
RefSeq	NP_828377.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1211688.
GenomeReviews	Gene locus SAV_7201 in contig BA000030_GR.
KEGG	sma:SAV_7201.
NMPDR	fig|227882.1.peg.7203.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q826J2.
OMA	CYFIPAF.
Enzyme and pathway databases
BioCyc	SAVE227882:SAV7201-MON.
BRENDA	2.7.1.30. 140873.
Family and domain databases
HAMAP	MF_00186. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK3_STRAW

Accession

Primary (citable) accession number: Q826J2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)