Alpha-1,3-galactosidase A precursor - Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Alpha-1,3-galactosidase A
EC=3.2.1.n1

Alternative name(s):
Exo-alpha-galactosidase A
EC=3.2.1.22
SaGal110A

Gene names

Name:	glaA
Ordered Locus Names:	SAV_7268

Organism

Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680) [Complete proteome] [HAMAP]

Taxonomic identifier

227882 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces ›

Protein attributes

Sequence length	625 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function

Alpha-galactosidase that specifically removes branched alpha-1,3-linked galactose residues present in blood group B antigens. Has no activity toward linear alpha-1,3-linked galactose residues.

Catalytic activity

Hydrolysis of terminal, non-reducing branched (1->3)-alpha-D-galactosidic residues, producing free D-galactose. Ref.4

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. Ref.4

Sequence similarities

Belongs to the glycosyl hydrolase 110 family. A subfamily.

Contains 5 PbH1 repeats.

Ontologies

Keywords
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular_function	raffinose alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 32

32

Potential

Chain

33 – 625

593

Alpha-1,3-galactosidase A

PRO_0000348474

Regions

Repeat

342 – 364

23

PbH1 1

Repeat

460 – 482

23

PbH1 2

Repeat

483 – 505

23

PbH1 3

Repeat

516 – 537

22

PbH1 4

Repeat

573 – 611

39

PbH1 5

Sequences

Sequence

Length

Mass (Da)

Tools

Q826C5 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 4B80A1A6C94D372E
Show »

FASTA

625

67,184

        10         20         30         40         50         60 
MAHGCSGGAM SRFVFLGVAL ALLGGATSPA AAAPRVTPVV VDVDDYGADP TGRTDSTPAV 

        70         80         90        100        110        120 
AAALRHAKSV DRPVRIVFSK GTYQLYPERA ETRELYMSNT VGADQRYRDK KIGLLVEDMH 

       130        140        150        160        170        180 
DVTVDGGGAK LVHHGLQTAF ASIRSTDVTF QNFSFDYAAP EVIDATVATT GVTDGHAYRV 

       190        200        210        220        230        240 
LKIPAGSPYR VNGTHITWLG ETSPATGQPY WSGVDGLQYT QIHDPEAQRT WRGDNPLFND 

       250        260        270        280        290        300 
VAAVTDLGGR RIRIDYTTAA RPADAGLVYQ MRLIERTEPG AFIWESKNVT MRSMNAYYLQ 

       310        320        330        340        350        360 
SFGVVGQFSE NISIDKVNFA PDPRSGRSTA SFADFVQMSG VKGKVSITRS LFDGPHDDPI 

       370        380        390        400        410        420 
NIHGTYLEVV GKPGPSTLTL AYKHPQTAGF PQFAPGDEVE FATKRTMTPL ADAHAQVTAV 

       430        440        450        460        470        480 
DGPSGMDHTK PLTTMTVTFD RPVPAGVETG GTVVENITAT PSVVISGNVF RNVPTRGILV 

       490        500        510        520        530        540 
TTRKPVLITG NRFDGMSMAS IYVSADAYQW YESGPVADLT IRGNSFTRPS GPVIFVEPTN 

       550        560        570        580        590        600 
QVIDPATPVH HNISVEHNSF DIGDVTVVNA KSVGGFAFTG NTVRRLDGAD HPPYTSPLFV 

       610        620 
FHGSSGIRIA RNHYDKGLNT SVVTD

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bacterial glycosidases for the production of universal red blood cells." Liu Q.P., Sulzenbacher G., Yuan H., Bennett E.P., Pietz G., Saunders K., Spence J., Nudelman E., Levery S.B., White T., Neveu J.M., Lane W.S., Bourne Y., Olsson M.L., Henrissat B., Clausen H. Nat. Biotechnol. 25:454-464(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.
[2]	"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites." Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.
[3]	"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis." Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. Nat. Biotechnol. 21:526-531(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.
[4]	"Identification of a GH110 subfamily of alpha1,3-galactosidases: novel enzymes for removal of the alpha3Gal xenotransplantation antigen." Liu Q.P., Yuan H., Bennett E.P., Levery S.B., Nudelman E., Spence J., Pietz G., Saunders K., White T., Olsson M.L., Henrissat B., Sulzenbacher G., Clausen H. J. Biol. Chem. 283:8545-8554(2008) [PubMed] [Europe PMC] [Abstract] Cited for: ENZYME ACTIVITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM109953 Genomic DNA. Translation: CAJ33349.1. BA000030 Genomic DNA. Translation: BAC74979.1.
RefSeq	NP_828444.1. NC_003155.4.
3D structure databases
ProteinModelPortal	Q826C5.
ModBase	Search...
Protein-protein interaction databases
STRING	227882.SAV_7268.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC74979; BAC74979; SAV_7268.
GeneID	1217418.
KEGG	sma:SAV_7268.
PATRIC	23728724. VBIStrAve112782_7700.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG77539.
HOGENOM	HOG000049734.
OMA	LKLRFMH.
ProtClustDB	CLSK823424.
Enzyme and pathway databases
BioCyc	SAVE227882:GJU1-7365-MONOMER.
Family and domain databases
Gene3D	2.160.20.10. 3 hits.
InterPro	IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
SMART	SM00710. PbH1. 5 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLAA_STRAW

Accession

Primary (citable) accession number: Q826C5
Secondary accession number(s): A4Q8G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents