Q826C5 (GLAA_STRAW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,3-galactosidase A
EC=3.2.1.n1

Alternative name(s):
Exo-alpha-galactosidase A
EC=3.2.1.22
SaGal110A

Gene names

Name:	glaA
Ordered Locus Names:	SAV_7268

Organism

Streptomyces avermitilis [Complete proteome] [HAMAP]

Taxonomic identifier

33903 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	625 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Alpha-galactosidase that specifically removes branched alpha-1,3-linked galactose residues present in blood group B antigens. Has no activity toward linear alpha-1,3-linked galactose residues.
Catalytic activity	Hydrolysis of terminal, non-reducing branched (1->3)-alpha-D-galactosidic residues, producing free D-galactose. Ref.4 Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. Ref.4
Sequence similarities	Belongs to the glycosyl hydrolase 110 family. A subfamily. Contains 5 PbH1 repeats.

Ontologies

Keywords
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	raffinose alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Potential
	Chain	33 – 625	593	Alpha-1,3-galactosidase A		PRO_0000348474
Regions
	Repeat	342 – 364	23	PbH1 1
	Repeat	460 – 482	23	PbH1 2
	Repeat	483 – 505	23	PbH1 3
	Repeat	516 – 537	22	PbH1 4
	Repeat	573 – 611	39	PbH1 5

Sequences

	Sequence		Length	Mass (Da)
	Q826C5 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 4B80A1A6C94D372E	FASTA	625	67,184
	10 20 30 40 50 60 MAHGCSGGAM SRFVFLGVAL ALLGGATSPA AAAPRVTPVV VDVDDYGADP TGRTDSTPAV 70 80 90 100 110 120 AAALRHAKSV DRPVRIVFSK GTYQLYPERA ETRELYMSNT VGADQRYRDK KIGLLVEDMH 130 140 150 160 170 180 DVTVDGGGAK LVHHGLQTAF ASIRSTDVTF QNFSFDYAAP EVIDATVATT GVTDGHAYRV 190 200 210 220 230 240 LKIPAGSPYR VNGTHITWLG ETSPATGQPY WSGVDGLQYT QIHDPEAQRT WRGDNPLFND 250 260 270 280 290 300 VAAVTDLGGR RIRIDYTTAA RPADAGLVYQ MRLIERTEPG AFIWESKNVT MRSMNAYYLQ 310 320 330 340 350 360 SFGVVGQFSE NISIDKVNFA PDPRSGRSTA SFADFVQMSG VKGKVSITRS LFDGPHDDPI 370 380 390 400 410 420 NIHGTYLEVV GKPGPSTLTL AYKHPQTAGF PQFAPGDEVE FATKRTMTPL ADAHAQVTAV 430 440 450 460 470 480 DGPSGMDHTK PLTTMTVTFD RPVPAGVETG GTVVENITAT PSVVISGNVF RNVPTRGILV 490 500 510 520 530 540 TTRKPVLITG NRFDGMSMAS IYVSADAYQW YESGPVADLT IRGNSFTRPS GPVIFVEPTN 550 560 570 580 590 600 QVIDPATPVH HNISVEHNSF DIGDVTVVNA KSVGGFAFTG NTVRRLDGAD HPPYTSPLFV 610 620 FHGSSGIRIA RNHYDKGLNT SVVTD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bacterial glycosidases for the production of universal red blood cells." Liu Q.P., Sulzenbacher G., Yuan H., Bennett E.P., Pietz G., Saunders K., Spence J., Nudelman E., Levery S.B., White T., Neveu J.M., Lane W.S., Bourne Y., Olsson M.L., Henrissat B., Clausen H. Nat. Biotechnol. 25:454-464(2007) [PubMed: 17401360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.
[2]	"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites." Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed: 11572948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.
[3]	"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis." Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. Nat. Biotechnol. 21:526-531(2003) [PubMed: 12692562] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.
[4]	"Identification of a GH110 subfamily of alpha1,3-galactosidases: novel enzymes for removal of the alpha3Gal xenotransplantation antigen." Liu Q.P., Yuan H., Bennett E.P., Levery S.B., Nudelman E., Spence J., Pietz G., Saunders K., White T., Olsson M.L., Henrissat B., Sulzenbacher G., Clausen H. J. Biol. Chem. 283:8545-8554(2008) [PubMed: 18227066] [Abstract] Cited for: ENZYME ACTIVITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM109953 Genomic DNA. Translation: CAJ33349.1. BA000030 Genomic DNA. Translation: BAC74979.1.
RefSeq	NP_828444.1. NC_003155.4.
3D structure databases
ProteinModelPortal	Q826C5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1217418.
GenomeReviews	Gene locus SAV_7268 in contig BA000030_GR.
KEGG	sma:SAV_7268.
NMPDR	fig|227882.1.peg.7270.
PATRIC	23728724. VBIStrAve112782_7700.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LKLRFMH.
ProtClustDB	CLSK823424.
Enzyme and pathway databases
BioCyc	SAVE227882:SAV7268-MONOMER.
Family and domain databases
InterPro	IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 3 hits.
SMART	SM00710. PbH1. 5 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLAA_STRAW

Accession

Primary (citable) accession number: Q826C5
Secondary accession number(s): A4Q8G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program