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Q825U9

- DEF3_STRAW

UniProt

Q825U9 - DEF3_STRAW

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Protein

Peptide deformylase 3

Gene

def3

Organism
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.UniRule annotation

Catalytic activityi

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe(2+) ion.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi135 – 1351IronUniRule annotation
Metal bindingi177 – 1771IronUniRule annotation
Active sitei178 – 1781UniRule annotation
Metal bindingi181 – 1811IronUniRule annotation

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. peptide deformylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciSAVE227882:GJU1-7446-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide deformylase 3UniRule annotation (EC:3.5.1.88UniRule annotation)
Short name:
PDF 3UniRule annotation
Alternative name(s):
Polypeptide deformylase 3UniRule annotation
Gene namesi
Name:def3UniRule annotation
Ordered Locus Names:SAV_7349
OrganismiStreptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680)
Taxonomic identifieri227882 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces
ProteomesiUP000000428: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 224224Peptide deformylase 3PRO_0000082851Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi227882.SAV_7349.

Structurei

3D structure databases

ProteinModelPortaliQ825U9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the polypeptide deformylase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0242.
HOGENOMiHOG000243508.
KOiK01462.
OMAiGWQARIV.
OrthoDBiEOG664CMF.

Family and domain databases

Gene3Di3.90.45.10. 1 hit.
HAMAPiMF_00163. Pep_deformylase.
InterProiIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
PANTHERiPTHR10458. PTHR10458. 1 hit.
PfamiPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFiPIRSF004749. Pep_def. 1 hit.
PRINTSiPR01576. PDEFORMYLASE.
SUPFAMiSSF56420. SSF56420. 1 hit.

Sequencei

Sequence statusi: Complete.

Q825U9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASESDRTVP DTRSASRPLA DRVEELLATG GPLPIVAAGD PVLRRGAEPY
60 70 80 90 100
DGQLGPGLLA RFVEALRLTM HAAPGVGLAA PQVGVGLRIA VIEDPAPVPE
110 120 130 140 150
EVGAVRGRVP QPFRVLVNPS YEAVGSDRAA FFEGCLSVPG WQAVVARPAR
160 170 180 190 200
VRLTALDEHG RAVDEEFTGW PARIVQHETD HLDGMLYLDR AELRSLSSNE
210 220
AMALRWSQPT PERAAAALGF ALPD
Length:224
Mass (Da):23,892
Last modified:June 1, 2003 - v1
Checksum:iD0ED0E6FB0806A23
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000030 Genomic DNA. Translation: BAC75060.1.
RefSeqiNP_828525.1. NC_003155.4.
WP_010988744.1. NC_003155.4.

Genome annotation databases

EnsemblBacteriaiBAC75060; BAC75060; SAV_7349.
GeneIDi1211719.
KEGGisma:SAV_7349.
PATRICi23728890. VBIStrAve112782_7783.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000030 Genomic DNA. Translation: BAC75060.1 .
RefSeqi NP_828525.1. NC_003155.4.
WP_010988744.1. NC_003155.4.

3D structure databases

ProteinModelPortali Q825U9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 227882.SAV_7349.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC75060 ; BAC75060 ; SAV_7349 .
GeneIDi 1211719.
KEGGi sma:SAV_7349.
PATRICi 23728890. VBIStrAve112782_7783.

Phylogenomic databases

eggNOGi COG0242.
HOGENOMi HOG000243508.
KOi K01462.
OMAi GWQARIV.
OrthoDBi EOG664CMF.

Enzyme and pathway databases

BioCyci SAVE227882:GJU1-7446-MONOMER.

Family and domain databases

Gene3Di 3.90.45.10. 1 hit.
HAMAPi MF_00163. Pep_deformylase.
InterProi IPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view ]
PANTHERi PTHR10458. PTHR10458. 1 hit.
Pfami PF01327. Pep_deformylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF004749. Pep_def. 1 hit.
PRINTSi PR01576. PDEFORMYLASE.
SUPFAMi SSF56420. SSF56420. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites."
    Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M.
    Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.
  2. "Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis."
    Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S.
    Nat. Biotechnol. 21:526-531(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.

Entry informationi

Entry nameiDEF3_STRAW
AccessioniPrimary (citable) accession number: Q825U9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: November 26, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3