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Protein

1,4-alpha-glucan branching enzyme GlgB 2

Gene

glgB2

Organism
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei274NucleophileUniRule annotation1
Active sitei327Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

UniPathwayiUPA00164.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB 2UniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2UniRule annotation
Alpha-(1->4)-glucan branching enzyme 2UniRule annotation
Glycogen branching enzyme 2UniRule annotation
Short name:
BE 2UniRule annotation
Gene namesi
Name:glgB2UniRule annotation
Ordered Locus Names:SAV_7399
OrganismiStreptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
Taxonomic identifieri227882 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces
Proteomesi
  • UP000000428 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001887481 – 5921,4-alpha-glucan branching enzyme GlgB 2Add BLAST592

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi227882.SAV_7399.

Structurei

3D structure databases

ProteinModelPortaliQ825Q8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9C. Bacteria.
COG0296. LUCA.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDDADHNT.
OrthoDBiPOG091H08D1.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 2 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q825Q8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMTHAGVLGT RFTVWAPNAR GVRVCGNFCR WDGAAFPMRS LGSSGVWELF
60 70 80 90 100
VPGIGEGELY KFEITRPDGT HTVRADPMAR RAEVPPATAS IVTESSYAWA
110 120 130 140 150
DGAWMAARGE RPVHESPFSV YEVHLPSWRP GLTYRQLAEQ LPAYVADLGF
160 170 180 190 200
THVELLPVAE HPFGGSWGYQ VTGFYAPTAR LGTPDDFKYL VDALHRAGVG
210 220 230 240 250
VLMDWVPAHF PRDDWALAEF DGRPLYEPED PQRAAHPDWG TLEFDYGRKE
260 270 280 290 300
VRNFLVANAV YWCEEFHIDG LRVDAVASML YLDYSREEGQ WSPNEFGGRD
310 320 330 340 350
NPDAVAFLQE MNATLYRRVP GVITIAEEST AWDGVTRATH DNGLGFGLKW
360 370 380 390 400
NMGWMHDSLG YVQHEPVHRK YHHHEMTFSM VYAYSENYVL PISHDEVVHG
410 420 430 440 450
KQALVSKMPG DWWQRRANHR AYLGFMWAHP GKQLLFMGQE FAQGAEWSPE
460 470 480 490 500
HGPEWWLLDD EYHSAGDHRG MRDLVRDLNT LYRAEPALWE RDTDPSGFAW
510 520 530 540 550
VVGDAAEDNV FAFLRHAADG TPLLAVSNFS PVVRHDYRLG VPDDIPAWQE
560 570 580 590
ALNTDAARYG GSDLICPDPV KPESGEIRLT LPPLATVWLR PA
Length:592
Mass (Da):66,806
Last modified:June 1, 2003 - v1
Checksum:i3A7355E0360BCC51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000030 Genomic DNA. Translation: BAC75110.1.
RefSeqiWP_010988794.1. NC_003155.5.

Genome annotation databases

EnsemblBacteriaiBAC75110; BAC75110; SAVERM_7399.
KEGGisma:SAV_7399.
PATRICi23728994. VBIStrAve112782_7835.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000030 Genomic DNA. Translation: BAC75110.1.
RefSeqiWP_010988794.1. NC_003155.5.

3D structure databases

ProteinModelPortaliQ825Q8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi227882.SAV_7399.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC75110; BAC75110; SAVERM_7399.
KEGGisma:SAV_7399.
PATRICi23728994. VBIStrAve112782_7835.

Phylogenomic databases

eggNOGiENOG4105C9C. Bacteria.
COG0296. LUCA.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDDADHNT.
OrthoDBiPOG091H08D1.

Enzyme and pathway databases

UniPathwayiUPA00164.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 2 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLGB2_STRAW
AccessioniPrimary (citable) accession number: Q825Q8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.