Q825Q8 (GLGB2_STRAW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB 2
EC=2.4.1.18

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2
Alpha-(1->4)-glucan branching enzyme 2
Glycogen branching enzyme 2
Short name=BE 2

Gene names

Name:	glgB2
Ordered Locus Names:	SAV_7399

Organism

Streptomyces avermitilis [Complete proteome] [HAMAP]

Taxonomic identifier

33903 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis Glycogen metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 592	592	1,4-alpha-glucan branching enzyme GlgB 2 HAMAP MF_00685		PRO_0000188748
Sites
	Active site	274	1	Nucleophile By similarity
	Active site	327	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q825Q8 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 3A7355E0360BCC51	FASTA	592	66,806
	10 20 30 40 50 60 MMTHAGVLGT RFTVWAPNAR GVRVCGNFCR WDGAAFPMRS LGSSGVWELF VPGIGEGELY 70 80 90 100 110 120 KFEITRPDGT HTVRADPMAR RAEVPPATAS IVTESSYAWA DGAWMAARGE RPVHESPFSV 130 140 150 160 170 180 YEVHLPSWRP GLTYRQLAEQ LPAYVADLGF THVELLPVAE HPFGGSWGYQ VTGFYAPTAR 190 200 210 220 230 240 LGTPDDFKYL VDALHRAGVG VLMDWVPAHF PRDDWALAEF DGRPLYEPED PQRAAHPDWG 250 260 270 280 290 300 TLEFDYGRKE VRNFLVANAV YWCEEFHIDG LRVDAVASML YLDYSREEGQ WSPNEFGGRD 310 320 330 340 350 360 NPDAVAFLQE MNATLYRRVP GVITIAEEST AWDGVTRATH DNGLGFGLKW NMGWMHDSLG 370 380 390 400 410 420 YVQHEPVHRK YHHHEMTFSM VYAYSENYVL PISHDEVVHG KQALVSKMPG DWWQRRANHR 430 440 450 460 470 480 AYLGFMWAHP GKQLLFMGQE FAQGAEWSPE HGPEWWLLDD EYHSAGDHRG MRDLVRDLNT 490 500 510 520 530 540 LYRAEPALWE RDTDPSGFAW VVGDAAEDNV FAFLRHAADG TPLLAVSNFS PVVRHDYRLG 550 560 570 580 590 VPDDIPAWQE ALNTDAARYG GSDLICPDPV KPESGEIRLT LPPLATVWLR PA

References

[1]

"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites." Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed: 11572948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

[2]

"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis." Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S. Nat. Biotechnol. 21:526-531(2003) [PubMed: 12692562] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000030 Genomic DNA. Translation: BAC75110.1.
RefSeq	NP_828575.1. NC_003155.4.
3D structure databases
ProteinModelPortal	Q825Q8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1217526.
GenomeReviews	Gene locus SAV_7399 in contig BA000030_GR.
KEGG	sma:SAV_7399.
NMPDR	fig|227882.1.peg.7401.
PATRIC	23728994. VBIStrAve112782_7835.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287139.
OMA	DGTCLYE.
ProtClustDB	PRK05402.
Enzyme and pathway databases
BioCyc	SAVE227882:SAV7399-MONOMER.
Family and domain databases
HAMAP	MF_00685. GlgB. Divergent sequence. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB2_STRAW

Accession

Primary (citable) accession number: Q825Q8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program