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Q825Q8 (GLGB2_STRAW) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB 2

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2
Alpha-(1->4)-glucan branching enzyme 2
Glycogen branching enzyme 2
Short name=BE 2
Gene names
Name:glgB2
Ordered Locus Names:SAV_7399
OrganismStreptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680) [Complete proteome] [HAMAP]
Taxonomic identifier227882 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length592 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5925921,4-alpha-glucan branching enzyme GlgB 2 HAMAP-Rule MF_00685
PRO_0000188748

Sites

Active site2741Nucleophile By similarity
Active site3271Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q825Q8 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 3A7355E0360BCC51

FASTA59266,806
        10         20         30         40         50         60 
MMTHAGVLGT RFTVWAPNAR GVRVCGNFCR WDGAAFPMRS LGSSGVWELF VPGIGEGELY 

        70         80         90        100        110        120 
KFEITRPDGT HTVRADPMAR RAEVPPATAS IVTESSYAWA DGAWMAARGE RPVHESPFSV 

       130        140        150        160        170        180 
YEVHLPSWRP GLTYRQLAEQ LPAYVADLGF THVELLPVAE HPFGGSWGYQ VTGFYAPTAR 

       190        200        210        220        230        240 
LGTPDDFKYL VDALHRAGVG VLMDWVPAHF PRDDWALAEF DGRPLYEPED PQRAAHPDWG 

       250        260        270        280        290        300 
TLEFDYGRKE VRNFLVANAV YWCEEFHIDG LRVDAVASML YLDYSREEGQ WSPNEFGGRD 

       310        320        330        340        350        360 
NPDAVAFLQE MNATLYRRVP GVITIAEEST AWDGVTRATH DNGLGFGLKW NMGWMHDSLG 

       370        380        390        400        410        420 
YVQHEPVHRK YHHHEMTFSM VYAYSENYVL PISHDEVVHG KQALVSKMPG DWWQRRANHR 

       430        440        450        460        470        480 
AYLGFMWAHP GKQLLFMGQE FAQGAEWSPE HGPEWWLLDD EYHSAGDHRG MRDLVRDLNT 

       490        500        510        520        530        540 
LYRAEPALWE RDTDPSGFAW VVGDAAEDNV FAFLRHAADG TPLLAVSNFS PVVRHDYRLG 

       550        560        570        580        590 
VPDDIPAWQE ALNTDAARYG GSDLICPDPV KPESGEIRLT LPPLATVWLR PA 

« Hide

References

[1]"Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites."
Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M.
Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.
[2]"Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis."
Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S.
Nat. Biotechnol. 21:526-531(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000030 Genomic DNA. Translation: BAC75110.1.
RefSeqNP_828575.1. NC_003155.4.

3D structure databases

ProteinModelPortalQ825Q8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING227882.SAV_7399.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC75110; BAC75110; SAV_7399.
GeneID1217526.
KEGGsma:SAV_7399.
PATRIC23728994. VBIStrAve112782_7835.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAISEGRHE.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycSAVE227882:GJU1-7496-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB2_STRAW
AccessionPrimary (citable) accession number: Q825Q8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: June 1, 2003
Last modified: June 11, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries