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Q82571

- RDRP_I47A0

UniProt

Q82571 - RDRP_I47A0

Protein

RNA-directed RNA polymerase catalytic subunit

Gene

PB1

Organism
Influenza A virus (strain A/Fort Monmouth/1/1947 H1N1)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    RNA-dependent RNA polymerase which is responsible for replication and transcription of virus segments. Binds the promoter sequence of the encapsidated viral RNA. Displays an endonuclease activity involved in cap-stealing. Cleaves cellular pre-mRNA to generate primers for viral transcription.

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    GO - Molecular functioni

    1. nucleotide binding Source: UniProtKB-KW
    2. RNA-directed RNA polymerase activity Source: UniProtKB-KW

    GO - Biological processi

    1. suppression by virus of host RNA polymerase II activity Source: UniProtKB-KW
    2. transcription, DNA-templated Source: InterPro
    3. viral RNA genome replication Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

    Keywords - Biological processi

    Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus, Viral RNA replication

    Keywords - Ligandi

    Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA-directed RNA polymerase catalytic subunit (EC:2.7.7.48)
    Alternative name(s):
    Polymerase basic protein 1
    Short name:
    PB1
    RNA-directed RNA polymerase subunit P1
    Gene namesi
    Name:PB1
    OrganismiInfluenza A virus (strain A/Fort Monmouth/1/1947 H1N1)
    Taxonomic identifieri380282 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
    Virus hostiAves [TaxID: 8782]
    Homo sapiens (Human) [TaxID: 9606]
    Sus scrofa (Pig) [TaxID: 9823]

    Subcellular locationi

    Host nucleus Curated

    GO - Cellular componenti

    1. host cell nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 757757RNA-directed RNA polymerase catalytic subunitPRO_0000078749Add
    BLAST

    Interactioni

    Subunit structurei

    Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini286 – 483198RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni249 – 2568Promoter-binding siteBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi187 – 1959Nuclear localization signalBy similarity
    Motifi203 – 21614Nuclear localization signalBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR007099. RNA-dir_pol_NSvirus.
    IPR001407. RNA_pol_PB1_influenza.
    [Graphical view]
    PfamiPF00602. Flu_PB1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000827. RdRPol_OMV. 1 hit.
    PROSITEiPS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q82571-1 [UniParc]FASTAAdd to Basket

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    MDVNPTLLFL KVPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS    50
    ERGRWTTNTE TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP 100
    GIFENSCIET MEVVQQTRVD KLTQGRQTYD WTLNRNQPAA TALANTIEVF 150
    RSNGLTANES GRLIDFLKDV MKSMDKEEME ITTHFQRKRR VRDNVTKKMV 200
    TQRTIGKRKQ RLNKRSYLIR ALTLNTMTKD AERGKLKRRA IATPGMQIRG 250
    FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTEISF 300
    TITGDNTKWN ENQNPRMFLA MITYMTRNQP EWFRNVLSIA PIMFSNKMAR 350
    LGKGYMFESK SMKLRTQIPA EMLANIDLKY FNDSTRKKIE KIRPLLIDGT 400
    ASLSPGMMMG MFNMLSTVLG VSILNLGQKR YTKTTYWRDG LQSSDDFALI 450
    VNAPNHEGIQ AGVDRFYRTC KLLGINMSKK KSYINRTGTF EFTSFFYRYG 500
    FEANFSMELP SFGVSGINES ADMSIGVTVI KNNMINNDLG PATAQMALQL 550
    FIKDYRYTYR CHRGDTQIQT RRSFEIKKLW EQTRSKAGLL VSDGGSNLYN 600
    IRNLHIPEVC LKWELMDEDY RGRLCNPLNP FVSHKEIESV NNAVMMPAHG 650
    PAKNMEYDAV ATTHSWIPKR NRTILNTSQR GILEDEQMYQ RCCNLFEKFF 700
    PSSSYRRPVG ISSMVEAMVS RARIDARIDF ESGRIKKEEF TEIMKICSTI 750
    EELRRQK 757
    Length:757
    Mass (Da):86,671
    Last modified:November 1, 1996 - v1
    Checksum:i7E45D6DE764A7534
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti154 – 1541G → D in strain: A/Fort Monmouth/1/1947-MA.
    Natural varianti538 – 5381D → G in strain: A/Fort Monmouth/1/1947-MA.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X99037 Genomic RNA. Translation: CAA67498.1.
    X99038 Genomic RNA. Translation: CAA67499.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X99037 Genomic RNA. Translation: CAA67498.1 .
    X99038 Genomic RNA. Translation: CAA67499.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR007099. RNA-dir_pol_NSvirus.
    IPR001407. RNA_pol_PB1_influenza.
    [Graphical view ]
    Pfami PF00602. Flu_PB1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000827. RdRPol_OMV. 1 hit.
    PROSITEi PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mutations in the hemagglutinin and matrix genes of a virulent influenza virus variant, A/FM/1/47-MA, control different stages in pathogenesis."
      Smeenk C.A., Wright K.E., Burns B.F., Thaker A.J., Brown E.G.
      Virus Res. 44:79-95(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: A/Fort Monmouth/1/1947 and A/Fort Monmouth/1/1947-MA.

    Entry informationi

    Entry nameiRDRP_I47A0
    AccessioniPrimary (citable) accession number: Q82571
    Secondary accession number(s): Q82572
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3