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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Chlamydophila caviae (strain GPIC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei44Substrate; via amide nitrogenUniRule annotation1
Binding sitei118ATPUniRule annotation1
Binding sitei166ATPUniRule annotation1
Binding sitei193Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:CCA_00204
OrganismiChlamydophila caviae (strain GPIC)
Taxonomic identifieri227941 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
Proteomesi
  • UP000002193 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000215021 – 262Hydroxyethylthiazole kinaseAdd BLAST262

Proteomic databases

PRIDEiQ824E8.

Interactioni

Protein-protein interaction databases

STRINGi227941.CCA00204.

Structurei

3D structure databases

ProteinModelPortaliQ824E8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4U. Bacteria.
COG2145. LUCA.
KOiK00878.
OMAiSPVMAHA.
OrthoDBiPOG091H004S.

Family and domain databases

CDDicd01170. THZ_kinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase. 1 hit.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.

Sequencei

Sequence statusi: Complete.

Q824E8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEQMYEALQ RLRKEKPVIL NITNYVSMDF LANCFLAIGA SPIMSVSDLE
60 70 80 90 100
LEELIGLSSA VYLNIGTLDH LFIQRSYRAV DIAIRQNKPI IFDPVGSGTT
110 120 130 140 150
KIRTEVSHHL LAHSTIVRGN ASKILSFGDL SIKTRGVDSA NTTHDAKETA
160 170 180 190 200
IALANECLCG CAIAVTGAVD FITDGKRSAT IELGDPLMSH VTGMGCSLTG
210 220 230 240 250
VLAAFRSVID DSFEAARLGV EYFSLCGMLA RERCEGPGLF KAYFLDELYA
260
ADFDRMRRYY EQ
Length:262
Mass (Da):28,765
Last modified:June 1, 2003 - v1
Checksum:iAD350E5415267F53
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015925 Genomic DNA. Translation: AAP04955.1.
RefSeqiWP_011006174.1. NC_003361.3.

Genome annotation databases

EnsemblBacteriaiAAP04955; AAP04955; CCA_00204.
KEGGicca:CCA_00204.
PATRICi20269430. VBIChlCav107360_0214.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015925 Genomic DNA. Translation: AAP04955.1.
RefSeqiWP_011006174.1. NC_003361.3.

3D structure databases

ProteinModelPortaliQ824E8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi227941.CCA00204.

Proteomic databases

PRIDEiQ824E8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP04955; AAP04955; CCA_00204.
KEGGicca:CCA_00204.
PATRICi20269430. VBIChlCav107360_0214.

Phylogenomic databases

eggNOGiENOG4105C4U. Bacteria.
COG2145. LUCA.
KOiK00878.
OMAiSPVMAHA.
OrthoDBiPOG091H004S.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.

Family and domain databases

CDDicd01170. THZ_kinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase. 1 hit.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHIM_CHLCV
AccessioniPrimary (citable) accession number: Q824E8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.