Reviewed, UniProtKB/Swiss-Prot Q823J9 (AMPA_CHLCV)

Names and origin

Protein names

Recommended name:
    Probable cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
      Short name=LAP
    Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	CCA_00411

Organism

Chlamydophila caviae [Complete proteome] [HAMAP]

Taxonomic identifier

83557 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydophila

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181
Cofactor	Binds 2 manganese ions per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_0000165737
Sites
	Active site	275	1	Potential
	Active site	349	1	Potential
	Metal binding	263	1	Manganese 2 By similarity
	Metal binding	268	1	Manganese 1 By similarity
	Metal binding	268	1	Manganese 2 By similarity
	Metal binding	286	1	Manganese 2 By similarity
	Metal binding	345	1	Manganese 1 By similarity
	Metal binding	347	1	Manganese 1 By similarity
	Metal binding	347	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q823J9-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: A5D7B39C5A887208	FASTA	499	54,363
	10 20 30 40 50 60 MVLFHSQASC SKRIEADAII LPFWKLKDKV KCAASIAKEY ESLYQVALDN FSAKSREVEL 70 80 90 100 110 120 IYSCGQGKEK RIVLLGLGKN EELSSQEVLE AYAKATRLLR KAKCTTVNVV LPTISELRIS 130 140 150 160 170 180 IEDFLTNLTS GILSLNYNYP KYTKESSNDP LLTKVNVLGI VPKIADRIFR KEENIFEGVY 190 200 210 220 230 240 LTRDLVNGNA DEVTPQKLAN IAKGMAKEFP SMDVKILNKD AILKEKMGLL AAVAKGSAVD 250 260 270 280 290 300 PCFIVLTYQG KPKSKDHTVL IGKGVTFDSG GLDLKPGKAM LTMKEDMAGA ATVLGILSGI 310 320 330 340 350 360 AALELPVNVT AIVPATENAI DAAAYKMGDV YTGMSGLSVE IGSTDAEGRL ILADAITYAL 370 380 390 400 410 420 KYCNPTRIID FATLTGAMVV SLGEDVAGFF SNNDVLAQDL SEASAETSEA LWRLPLVEKY 430 440 450 460 470 480 NKALHSDIAD MKNIGSNRAG AITAALFLKR FLEDQPVAWA HLDIAGTAYR EKDEDLYPKY 490 ASGFGVRCLI YYIEKFLSK

References

[1]

"Genome sequence of Chlamydophila caviae (Chlamydia psittaci GPIC): examining the role of niche-specific genes in the evolution of the Chlamydiaceae." Read T.D., Myers G.S.A., Brunham R.C., Nelson W.C., Paulsen I.T., Heidelberg J.F., Holtzapple E.K., Khouri H.M., Federova N.B., Carty H.A., Umayam L.A., Haft D.H., Peterson J.D., Beanan M.J., White O., Salzberg S.L., Hsia R.-C., McClarty G. , Rank R.G., Bavoil P.M., Fraser C.M. Nucleic Acids Res. 31:2134-2147(2003) [PubMed: 12682364] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GPIC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015925 Genomic DNA. Translation: AAP05157.1.
RefSeq	NP_829279.1.
3D structure databases
HSSP	HSSP built from PDB template 1BPN based on UniProtKB P00727.
ModBase	Search...
Protein family/group databases
MEROPS	M17.003.
Genome annotation databases
GeneID	1218096.
GenomeReviews	Gene locus CCA_00411 in contig AE015925_GR.
KEGG	cca:CCA00411.
TIGR	CCA_00411.
Phylogenomic databases
HOGENOM	Q823J9.
OMA	ASEFESK.
Enzyme and pathway databases
BioCyc	CCAV227941:CCA_00411-MON.
BRENDA	3.4.11.1. 304519.
Family and domain databases
HAMAP	MF_00181. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_CHLCV

Accession

Primary (citable) accession number: Q823J9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)