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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Chlamydophila caviae (strain GPIC)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei595ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCCAV227941:G1G03-674-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CCA_00664
OrganismiChlamydophila caviae (strain GPIC)
Taxonomic identifieri227941 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
Proteomesi
  • UP000002193 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000985311 – 1043Isoleucine--tRNA ligaseAdd BLAST1043

Proteomic databases

PRIDEiQ822L8

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi227941.CCA00664

Structurei

3D structure databases

ProteinModelPortaliQ822L8
SMRiQ822L8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi49 – 59"HIGH" regionAdd BLAST11
Motifi592 – 596"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q822L8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTEGESSKE SLANREERIL DFWKTHDIFQ KSLKNREGKT LYSFYDGPPF
60 70 80 90 100
ATGLPHYGHL LAGTIKDVVG RFATMDGYYV PRRFGWDCHG VPVEYEVEKS
110 120 130 140 150
LDLTTPGAIE DFGVAKFNEE CRKIVFRYVD EWERYVHRLG RWVDFSVTWK
160 170 180 190 200
TMDASFMESV WWVFRSLYDQ GLVYEGVKVV PFSTKLGTPL SNFEAGQNYK
210 220 230 240 250
EVDDPSVVIK FALHGDPASL LVWTTTPWTL VSNMAAAVGP EITYVRVADK
260 270 280 290 300
VSGEQWILGQ GCLSRWFSDP DSYEILESFL GTALVGKSYE PPFSFFEHKR
310 320 330 340 350
AEGAYKILSG SFVEESEGTG VVHMAPAFGE ADFFVCKEHH VPIVCPVDNH
360 370 380 390 400
GCFTEEIPEY QGQYIKSCDK GIIKSLKNLG KVFYHGTVMH RYPFCWRTDT
410 420 430 440 450
PLIYKTVNSW FVSVEKIKDK MLRANQKIHW VPEHIKEGRF GKWLDGARDW
460 470 480 490 500
AISRNRYWGT PIPIWKSKEG EILVIGSVKE LEELTGEKIS DLHCHFIDQL
510 520 530 540 550
KVEKEGKTFQ RVPYVFDCWF DSGAMPYAQN HYPFENQKET EAGFPADFIA
560 570 580 590 600
EGLDQTRGWF YTLTVISSAL FDQTAFKNAI VNGIVLAEDG NKMSKRLNNY
610 620 630 640 650
PSPMGIMNTY GADALRLYLL DSVVVKAEDL RFSDKGVESI LKQILLPLTN
660 670 680 690 700
VLSFFKTYTD LYGFDADNYD KEEITYSEID RWILSNLYTV VGKVRESMSS
710 720 730 740 750
YNLNTAVNPF VTFIDDLTNW YIRRCRRRFW ESEDTPDRRA AFATLYEVLT
760 770 780 790 800
VFCRVIAPFI PFISEDIYQQ IKTEHSAESV HLCDFPHIDL AKVFPDLEQR
810 820 830 840 850
MSDAREIVGL GHSLRKEHKL KVRQPLANFY IVGPKDRLDE LTSFEQLIAE
860 870 880 890 900
ELNVKNIVFY KETPSFVKTT VKPNFRSLGR KVGEKIKDVQ RALSNLSQDQ
910 920 930 940 950
IQQLLKQQYL LLNLGFEEIT LGIDDVVISW ETDPGYVARS SSLFTVVLDC
960 970 980 990 1000
QLTEDLIVEA ISRELVNKIN TMRRNHKLHV SDRILLQIHS SEDVEKAFLH
1010 1020 1030 1040
YEDYICEETL TVQFEFKDSV EGEEWDINGH PTVIALTVAS KAN
Length:1,043
Mass (Da):119,911
Last modified:June 1, 2003 - v1
Checksum:i39606F73FF6A6635
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015925 Genomic DNA Translation: AAP05406.1
RefSeqiWP_011006621.1, NC_003361.3

Genome annotation databases

EnsemblBacteriaiAAP05406; AAP05406; CCA_00664
KEGGicca:CCA_00664

Similar proteinsi

Entry informationi

Entry nameiSYI_CHLCV
AccessioniPrimary (citable) accession number: Q822L8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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