SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q822L8

- SYI_CHLCV

UniProt

Q822L8 - SYI_CHLCV

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Isoleucine--tRNA ligase
Gene
ileS, CCA_00664
Organism
Chlamydophila caviae (strain GPIC)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei595 – 5951ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCCAV227941:GH8L-690-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:CCA_00664
OrganismiChlamydophila caviae (strain GPIC)
Taxonomic identifieri227941 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydophila
ProteomesiUP000002193: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10431043Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098531Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi227941.CCA00664.

Structurei

3D structure databases

ProteinModelPortaliQ822L8.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi49 – 5911"HIGH" regionUniRule annotation
Add
BLAST
Motifi592 – 5965"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiENRDWFE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q822L8-1 [UniParc]FASTAAdd to Basket

« Hide

MNTEGESSKE SLANREERIL DFWKTHDIFQ KSLKNREGKT LYSFYDGPPF     50
ATGLPHYGHL LAGTIKDVVG RFATMDGYYV PRRFGWDCHG VPVEYEVEKS 100
LDLTTPGAIE DFGVAKFNEE CRKIVFRYVD EWERYVHRLG RWVDFSVTWK 150
TMDASFMESV WWVFRSLYDQ GLVYEGVKVV PFSTKLGTPL SNFEAGQNYK 200
EVDDPSVVIK FALHGDPASL LVWTTTPWTL VSNMAAAVGP EITYVRVADK 250
VSGEQWILGQ GCLSRWFSDP DSYEILESFL GTALVGKSYE PPFSFFEHKR 300
AEGAYKILSG SFVEESEGTG VVHMAPAFGE ADFFVCKEHH VPIVCPVDNH 350
GCFTEEIPEY QGQYIKSCDK GIIKSLKNLG KVFYHGTVMH RYPFCWRTDT 400
PLIYKTVNSW FVSVEKIKDK MLRANQKIHW VPEHIKEGRF GKWLDGARDW 450
AISRNRYWGT PIPIWKSKEG EILVIGSVKE LEELTGEKIS DLHCHFIDQL 500
KVEKEGKTFQ RVPYVFDCWF DSGAMPYAQN HYPFENQKET EAGFPADFIA 550
EGLDQTRGWF YTLTVISSAL FDQTAFKNAI VNGIVLAEDG NKMSKRLNNY 600
PSPMGIMNTY GADALRLYLL DSVVVKAEDL RFSDKGVESI LKQILLPLTN 650
VLSFFKTYTD LYGFDADNYD KEEITYSEID RWILSNLYTV VGKVRESMSS 700
YNLNTAVNPF VTFIDDLTNW YIRRCRRRFW ESEDTPDRRA AFATLYEVLT 750
VFCRVIAPFI PFISEDIYQQ IKTEHSAESV HLCDFPHIDL AKVFPDLEQR 800
MSDAREIVGL GHSLRKEHKL KVRQPLANFY IVGPKDRLDE LTSFEQLIAE 850
ELNVKNIVFY KETPSFVKTT VKPNFRSLGR KVGEKIKDVQ RALSNLSQDQ 900
IQQLLKQQYL LLNLGFEEIT LGIDDVVISW ETDPGYVARS SSLFTVVLDC 950
QLTEDLIVEA ISRELVNKIN TMRRNHKLHV SDRILLQIHS SEDVEKAFLH 1000
YEDYICEETL TVQFEFKDSV EGEEWDINGH PTVIALTVAS KAN 1043
Length:1,043
Mass (Da):119,911
Last modified:June 1, 2003 - v1
Checksum:i39606F73FF6A6635
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015925 Genomic DNA. Translation: AAP05406.1.
RefSeqiNP_829528.1. NC_003361.3.
WP_011006621.1. NC_003361.3.

Genome annotation databases

EnsemblBacteriaiAAP05406; AAP05406; CCA_00664.
GeneIDi1218349.
KEGGicca:CCA00664.
PATRICi20270441. VBIChlCav107360_0707.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015925 Genomic DNA. Translation: AAP05406.1 .
RefSeqi NP_829528.1. NC_003361.3.
WP_011006621.1. NC_003361.3.

3D structure databases

ProteinModelPortali Q822L8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 227941.CCA00664.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAP05406 ; AAP05406 ; CCA_00664 .
GeneIDi 1218349.
KEGGi cca:CCA00664.
PATRICi 20270441. VBIChlCav107360_0707.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi ENRDWFE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CCAV227941:GH8L-690-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Chlamydophila caviae (Chlamydia psittaci GPIC): examining the role of niche-specific genes in the evolution of the Chlamydiaceae."
    Read T.D., Myers G.S.A., Brunham R.C., Nelson W.C., Paulsen I.T., Heidelberg J.F., Holtzapple E.K., Khouri H.M., Federova N.B., Carty H.A., Umayam L.A., Haft D.H., Peterson J.D., Beanan M.J., White O., Salzberg S.L., Hsia R.-C., McClarty G.
    , Rank R.G., Bavoil P.M., Fraser C.M.
    Nucleic Acids Res. 31:2134-2147(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GPIC.

Entry informationi

Entry nameiSYI_CHLCV
AccessioniPrimary (citable) accession number: Q822L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2003
Last modified: September 3, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi