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Q822L8

- SYI_CHLCV

UniProt

Q822L8 - SYI_CHLCV

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Chlamydophila caviae (strain GPIC)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei595 – 5951ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCCAV227941:GH8L-690-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:CCA_00664
    OrganismiChlamydophila caviae (strain GPIC)
    Taxonomic identifieri227941 [NCBI]
    Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydophila
    ProteomesiUP000002193: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10431043Isoleucine--tRNA ligasePRO_0000098531Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi227941.CCA00664.

    Structurei

    3D structure databases

    ProteinModelPortaliQ822L8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi49 – 5911"HIGH" regionAdd
    BLAST
    Motifi592 – 5965"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiENRDWFE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q822L8-1 [UniParc]FASTAAdd to Basket

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    MNTEGESSKE SLANREERIL DFWKTHDIFQ KSLKNREGKT LYSFYDGPPF     50
    ATGLPHYGHL LAGTIKDVVG RFATMDGYYV PRRFGWDCHG VPVEYEVEKS 100
    LDLTTPGAIE DFGVAKFNEE CRKIVFRYVD EWERYVHRLG RWVDFSVTWK 150
    TMDASFMESV WWVFRSLYDQ GLVYEGVKVV PFSTKLGTPL SNFEAGQNYK 200
    EVDDPSVVIK FALHGDPASL LVWTTTPWTL VSNMAAAVGP EITYVRVADK 250
    VSGEQWILGQ GCLSRWFSDP DSYEILESFL GTALVGKSYE PPFSFFEHKR 300
    AEGAYKILSG SFVEESEGTG VVHMAPAFGE ADFFVCKEHH VPIVCPVDNH 350
    GCFTEEIPEY QGQYIKSCDK GIIKSLKNLG KVFYHGTVMH RYPFCWRTDT 400
    PLIYKTVNSW FVSVEKIKDK MLRANQKIHW VPEHIKEGRF GKWLDGARDW 450
    AISRNRYWGT PIPIWKSKEG EILVIGSVKE LEELTGEKIS DLHCHFIDQL 500
    KVEKEGKTFQ RVPYVFDCWF DSGAMPYAQN HYPFENQKET EAGFPADFIA 550
    EGLDQTRGWF YTLTVISSAL FDQTAFKNAI VNGIVLAEDG NKMSKRLNNY 600
    PSPMGIMNTY GADALRLYLL DSVVVKAEDL RFSDKGVESI LKQILLPLTN 650
    VLSFFKTYTD LYGFDADNYD KEEITYSEID RWILSNLYTV VGKVRESMSS 700
    YNLNTAVNPF VTFIDDLTNW YIRRCRRRFW ESEDTPDRRA AFATLYEVLT 750
    VFCRVIAPFI PFISEDIYQQ IKTEHSAESV HLCDFPHIDL AKVFPDLEQR 800
    MSDAREIVGL GHSLRKEHKL KVRQPLANFY IVGPKDRLDE LTSFEQLIAE 850
    ELNVKNIVFY KETPSFVKTT VKPNFRSLGR KVGEKIKDVQ RALSNLSQDQ 900
    IQQLLKQQYL LLNLGFEEIT LGIDDVVISW ETDPGYVARS SSLFTVVLDC 950
    QLTEDLIVEA ISRELVNKIN TMRRNHKLHV SDRILLQIHS SEDVEKAFLH 1000
    YEDYICEETL TVQFEFKDSV EGEEWDINGH PTVIALTVAS KAN 1043
    Length:1,043
    Mass (Da):119,911
    Last modified:June 1, 2003 - v1
    Checksum:i39606F73FF6A6635
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015925 Genomic DNA. Translation: AAP05406.1.
    RefSeqiNP_829528.1. NC_003361.3.
    WP_011006621.1. NC_003361.3.

    Genome annotation databases

    EnsemblBacteriaiAAP05406; AAP05406; CCA_00664.
    GeneIDi1218349.
    KEGGicca:CCA00664.
    PATRICi20270441. VBIChlCav107360_0707.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015925 Genomic DNA. Translation: AAP05406.1 .
    RefSeqi NP_829528.1. NC_003361.3.
    WP_011006621.1. NC_003361.3.

    3D structure databases

    ProteinModelPortali Q822L8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 227941.CCA00664.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAP05406 ; AAP05406 ; CCA_00664 .
    GeneIDi 1218349.
    KEGGi cca:CCA00664.
    PATRICi 20270441. VBIChlCav107360_0707.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi ENRDWFE.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CCAV227941:GH8L-690-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Chlamydophila caviae (Chlamydia psittaci GPIC): examining the role of niche-specific genes in the evolution of the Chlamydiaceae."
      Read T.D., Myers G.S.A., Brunham R.C., Nelson W.C., Paulsen I.T., Heidelberg J.F., Holtzapple E.K., Khouri H.M., Federova N.B., Carty H.A., Umayam L.A., Haft D.H., Peterson J.D., Beanan M.J., White O., Salzberg S.L., Hsia R.-C., McClarty G.
      , Rank R.G., Bavoil P.M., Fraser C.M.
      Nucleic Acids Res. 31:2134-2147(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GPIC.

    Entry informationi

    Entry nameiSYI_CHLCV
    AccessioniPrimary (citable) accession number: Q822L8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3