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Q822L8

- SYI_CHLCV

UniProt

Q822L8 - SYI_CHLCV

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Chlamydophila caviae (strain GPIC)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei595 – 5951ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCCAV227941:GH8L-690-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CCA_00664
OrganismiChlamydophila caviae (strain GPIC)
Taxonomic identifieri227941 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydophila
ProteomesiUP000002193: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10431043Isoleucine--tRNA ligasePRO_0000098531Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi227941.CCA00664.

Structurei

3D structure databases

ProteinModelPortaliQ822L8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi49 – 5911"HIGH" regionAdd
BLAST
Motifi592 – 5965"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiENRDWFE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q822L8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNTEGESSKE SLANREERIL DFWKTHDIFQ KSLKNREGKT LYSFYDGPPF
60 70 80 90 100
ATGLPHYGHL LAGTIKDVVG RFATMDGYYV PRRFGWDCHG VPVEYEVEKS
110 120 130 140 150
LDLTTPGAIE DFGVAKFNEE CRKIVFRYVD EWERYVHRLG RWVDFSVTWK
160 170 180 190 200
TMDASFMESV WWVFRSLYDQ GLVYEGVKVV PFSTKLGTPL SNFEAGQNYK
210 220 230 240 250
EVDDPSVVIK FALHGDPASL LVWTTTPWTL VSNMAAAVGP EITYVRVADK
260 270 280 290 300
VSGEQWILGQ GCLSRWFSDP DSYEILESFL GTALVGKSYE PPFSFFEHKR
310 320 330 340 350
AEGAYKILSG SFVEESEGTG VVHMAPAFGE ADFFVCKEHH VPIVCPVDNH
360 370 380 390 400
GCFTEEIPEY QGQYIKSCDK GIIKSLKNLG KVFYHGTVMH RYPFCWRTDT
410 420 430 440 450
PLIYKTVNSW FVSVEKIKDK MLRANQKIHW VPEHIKEGRF GKWLDGARDW
460 470 480 490 500
AISRNRYWGT PIPIWKSKEG EILVIGSVKE LEELTGEKIS DLHCHFIDQL
510 520 530 540 550
KVEKEGKTFQ RVPYVFDCWF DSGAMPYAQN HYPFENQKET EAGFPADFIA
560 570 580 590 600
EGLDQTRGWF YTLTVISSAL FDQTAFKNAI VNGIVLAEDG NKMSKRLNNY
610 620 630 640 650
PSPMGIMNTY GADALRLYLL DSVVVKAEDL RFSDKGVESI LKQILLPLTN
660 670 680 690 700
VLSFFKTYTD LYGFDADNYD KEEITYSEID RWILSNLYTV VGKVRESMSS
710 720 730 740 750
YNLNTAVNPF VTFIDDLTNW YIRRCRRRFW ESEDTPDRRA AFATLYEVLT
760 770 780 790 800
VFCRVIAPFI PFISEDIYQQ IKTEHSAESV HLCDFPHIDL AKVFPDLEQR
810 820 830 840 850
MSDAREIVGL GHSLRKEHKL KVRQPLANFY IVGPKDRLDE LTSFEQLIAE
860 870 880 890 900
ELNVKNIVFY KETPSFVKTT VKPNFRSLGR KVGEKIKDVQ RALSNLSQDQ
910 920 930 940 950
IQQLLKQQYL LLNLGFEEIT LGIDDVVISW ETDPGYVARS SSLFTVVLDC
960 970 980 990 1000
QLTEDLIVEA ISRELVNKIN TMRRNHKLHV SDRILLQIHS SEDVEKAFLH
1010 1020 1030 1040
YEDYICEETL TVQFEFKDSV EGEEWDINGH PTVIALTVAS KAN
Length:1,043
Mass (Da):119,911
Last modified:June 1, 2003 - v1
Checksum:i39606F73FF6A6635
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015925 Genomic DNA. Translation: AAP05406.1.
RefSeqiNP_829528.1. NC_003361.3.

Genome annotation databases

EnsemblBacteriaiAAP05406; AAP05406; CCA_00664.
GeneIDi1218349.
KEGGicca:CCA00664.
PATRICi20270441. VBIChlCav107360_0707.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015925 Genomic DNA. Translation: AAP05406.1 .
RefSeqi NP_829528.1. NC_003361.3.

3D structure databases

ProteinModelPortali Q822L8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 227941.CCA00664.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAP05406 ; AAP05406 ; CCA_00664 .
GeneIDi 1218349.
KEGGi cca:CCA00664.
PATRICi 20270441. VBIChlCav107360_0707.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi ENRDWFE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CCAV227941:GH8L-690-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Chlamydophila caviae (Chlamydia psittaci GPIC): examining the role of niche-specific genes in the evolution of the Chlamydiaceae."
    Read T.D., Myers G.S.A., Brunham R.C., Nelson W.C., Paulsen I.T., Heidelberg J.F., Holtzapple E.K., Khouri H.M., Federova N.B., Carty H.A., Umayam L.A., Haft D.H., Peterson J.D., Beanan M.J., White O., Salzberg S.L., Hsia R.-C., McClarty G.
    , Rank R.G., Bavoil P.M., Fraser C.M.
    Nucleic Acids Res. 31:2134-2147(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GPIC.

Entry informationi

Entry nameiSYI_CHLCV
AccessioniPrimary (citable) accession number: Q822L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2003
Last modified: October 29, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3