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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Chlamydophila caviae (strain GPIC)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei184SubstrateUniRule annotation1
Active sitei185Proton donor/acceptorUniRule annotation1
Active sitei315UniRule annotation1
Binding sitei316SubstrateUniRule annotation1
Sitei328Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processTricarboxylic acid cycle

Enzyme and pathway databases

BioCyciCCAV227941:G1G03-758-MONOMER
UniPathwayiUPA00223; UER01007

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotation
Ordered Locus Names:CCA_00748
OrganismiChlamydophila caviae (strain GPIC)
Taxonomic identifieri227941 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
Proteomesi
  • UP000002193 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001612661 – 460Fumarate hydratase class IIAdd BLAST460

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi227941.CCA00748

Structurei

3D structure databases

ProteinModelPortaliQ822D5
SMRiQ822D5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni95 – 97Substrate bindingUniRule annotation3
Regioni126 – 129Substrate binding (B site)UniRule annotation4
Regioni136 – 138Substrate bindingUniRule annotation3
Regioni321 – 323Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q Bacteria
COG0114 LUCA
KOiK01679
OMAiVSFTDNC
OrthoDBiPOG091H01XG

Family and domain databases

CDDicd01362 Fumarase_classII, 1 hit
Gene3Di1.10.275.101 hit
HAMAPiMF_00743 FumaraseC, 1 hit
InterProiView protein in InterPro
IPR005677 Fum_hydII
IPR024083 Fumarase/histidase_N
IPR018951 Fumarase_C_C
IPR020557 Fumarate_lyase_CS
IPR000362 Fumarate_lyase_fam
IPR022761 Fumarate_lyase_N
IPR008948 L-Aspartase-like
PANTHERiPTHR11444 PTHR11444, 1 hit
PfamiView protein in Pfam
PF10415 FumaraseC_C, 1 hit
PF00206 Lyase_1, 1 hit
PRINTSiPR00149 FUMRATELYASE
SUPFAMiSSF48557 SSF48557, 1 hit
TIGRFAMsiTIGR00979 fumC_II, 1 hit
PROSITEiView protein in PROSITE
PS00163 FUMARATE_LYASES, 1 hit

Sequencei

Sequence statusi: Complete.

Q822D5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQENDSLGI VEVPEDKLYG AQTARSQKYF SWAPEVMPKE VIRALVLIKQ
60 70 80 90 100
CAAKANHDLG FLDSKYCDMI VSAASEILEG GFEEHFPLKV WQTGSGTQSN
110 120 130 140 150
MNVNEVIANL AIQRHGGVVG SKTPIHPNDH VNKSQSSNDV FPTAMHIAAV
160 170 180 190 200
INLKKKLIPA MDHLQRALDA KVAEFRDCVK IGRTHLMDAV PMTLGQEFSG
210 220 230 240 250
YSNQIRQSLE RVAFSLTHMY ELAIGGTAVG TGLNVPNGFI DKVIHYLRQE
260 270 280 290 300
TGEPFVAASN YFSALSNHDT LVNAHGVLAT LACALTKIAT DLSFLGSGPR
310 320 330 340 350
CGLGELLFPE NEPGSSIMPG KINPTQCEAL QMVCAQVIGN NQAVIMGGSR
360 370 380 390 400
GNFELNVMKP VIIYNFLQSV DILAGGMQAF ADYFVSGLRV NKPRLKEYLD
410 420 430 440 450
NSLMLVTALT PVLGYDKCSK MALKAFHDNI NLKEACIQMG YLSAEEFDRL
460
VVPESMVGKF
Length:460
Mass (Da):50,259
Last modified:June 1, 2003 - v1
Checksum:i8F68DE2F0046AFDA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015925 Genomic DNA Translation: AAP05489.1
RefSeqiWP_011006703.1, NC_003361.3

Genome annotation databases

EnsemblBacteriaiAAP05489; AAP05489; CCA_00748
KEGGicca:CCA_00748

Similar proteinsi

Entry informationi

Entry nameiFUMC_CHLCV
AccessioniPrimary (citable) accession number: Q822D5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: February 28, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome