Reviewed, UniProtKB/Swiss-Prot Q822D5 (FUMC_CHLCV)

Names and origin

Protein names

Recommended name:
    Fumarate hydratase class II
      Short name=Fumarase C
    EC=4.2.1.2

Gene names

Name:	fumC
Ordered Locus Names:	CCA_00748

Organism

Chlamydophila caviae [Complete proteome] [HAMAP]

Taxonomic identifier

83557 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydophila

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle. HAMAP MF_00743
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 460	460	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161266
Regions
	Region	126 – 129	4	B site By similarity
	Region	136 – 138	3	Substrate binding By similarity
Sites
	Binding site	97	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q822D5-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 8F68DE2F0046AFDA	FASTA	460	50,259
	10 20 30 40 50 60 MRQENDSLGI VEVPEDKLYG AQTARSQKYF SWAPEVMPKE VIRALVLIKQ CAAKANHDLG 70 80 90 100 110 120 FLDSKYCDMI VSAASEILEG GFEEHFPLKV WQTGSGTQSN MNVNEVIANL AIQRHGGVVG 130 140 150 160 170 180 SKTPIHPNDH VNKSQSSNDV FPTAMHIAAV INLKKKLIPA MDHLQRALDA KVAEFRDCVK 190 200 210 220 230 240 IGRTHLMDAV PMTLGQEFSG YSNQIRQSLE RVAFSLTHMY ELAIGGTAVG TGLNVPNGFI 250 260 270 280 290 300 DKVIHYLRQE TGEPFVAASN YFSALSNHDT LVNAHGVLAT LACALTKIAT DLSFLGSGPR 310 320 330 340 350 360 CGLGELLFPE NEPGSSIMPG KINPTQCEAL QMVCAQVIGN NQAVIMGGSR GNFELNVMKP 370 380 390 400 410 420 VIIYNFLQSV DILAGGMQAF ADYFVSGLRV NKPRLKEYLD NSLMLVTALT PVLGYDKCSK 430 440 450 460 MALKAFHDNI NLKEACIQMG YLSAEEFDRL VVPESMVGKF

References

[1]

"Genome sequence of Chlamydophila caviae (Chlamydia psittaci GPIC): examining the role of niche-specific genes in the evolution of the Chlamydiaceae." Read T.D., Myers G.S.A., Brunham R.C., Nelson W.C., Paulsen I.T., Heidelberg J.F., Holtzapple E.K., Khouri H.M., Federova N.B., Carty H.A., Umayam L.A., Haft D.H., Peterson J.D., Beanan M.J., White O., Salzberg S.L., Hsia R.-C., McClarty G. , Rank R.G., Bavoil P.M., Fraser C.M. Nucleic Acids Res. 31:2134-2147(2003) [PubMed: 12682364] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GPIC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015925 Genomic DNA. Translation: AAP05489.1.
RefSeq	NP_829611.1.
3D structure databases
HSSP	HSSP built from PDB template 1FUQ based on UniProtKB P05042.
ModBase	Search...
Genome annotation databases
GeneID	1218433.
GenomeReviews	Gene locus CCA_00748 in contig AE015925_GR.
KEGG	cca:CCA00748.
TIGR	CCA_00748.
Phylogenomic databases
HOGENOM	Q822D5.
OMA	GSQGHFE.
Enzyme and pathway databases
BioCyc	CCAV227941:CCA_00748-MON.
BRENDA	4.2.1.2. 304519.
Family and domain databases
HAMAP	MF_00743. [Tree]
InterPro	IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00979. fumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_CHLCV

Accession

Primary (citable) accession number: Q822D5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)