Reviewed, UniProtKB/Swiss-Prot Q822B0 (MURB_CHLCV)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylenolpyruvoylglucosamine reductase
    EC=1.1.1.158
Alternative name(s):
    UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	CCA_00773

Organism

Chlamydophila caviae [Complete proteome] [HAMAP]

Taxonomic identifier

83557 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydophila

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000179194
Regions
	Domain	26 – 191	166	FAD-binding PCMH-type
Sites
	Active site	170	1	By similarity
	Active site	218	1	Proton donor By similarity
	Active site	287	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q822B0-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 4AE7B30C1EC4EF46	FASTA	296	32,713
	10 20 30 40 50 60 MKESTVIHFP FSVRRGVWLS KYSTFRIGGP ANYFKEVHST SEAQRVIQFL YSHNYPFIIV 70 80 90 100 110 120 GKGSNCLFDD QGFDGFVLYN SIQGKEFLSD TTIKAYSGMS FSFLGKTLAS SGYSGLEFAV 130 140 150 160 170 180 GIPGSVGGAI FMNAGTGKQD TASVVESVEV INSKGEILSY RNEELEFGYR KSRFQNHNEF 190 200 210 220 230 240 ILSATFRISK NSSSLQIAKD LLESRLLSQP YQQPSVGCIF RNPPGNCAGK LIDEAGLKGV 250 260 270 280 290 SLGGAQISLK HANFIVNTGR ATSHEVKELI RMVQDKLRSQ GISLEEEIRI IPYRLP

References

[1]

"Genome sequence of Chlamydophila caviae (Chlamydia psittaci GPIC): examining the role of niche-specific genes in the evolution of the Chlamydiaceae." Read T.D., Myers G.S.A., Brunham R.C., Nelson W.C., Paulsen I.T., Heidelberg J.F., Holtzapple E.K., Khouri H.M., Federova N.B., Carty H.A., Umayam L.A., Haft D.H., Peterson J.D., Beanan M.J., White O., Salzberg S.L., Hsia R.-C., McClarty G. , Rank R.G., Bavoil P.M., Fraser C.M. Nucleic Acids Res. 31:2134-2147(2003) [PubMed: 12682364] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GPIC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015925 Genomic DNA. Translation: AAP05514.1.
RefSeq	NP_829636.1.
3D structure databases
HSSP	HSSP built from PDB template 1HSK based on UniProtKB Q93G02.
ModBase	Search...
Genome annotation databases
GeneID	1218458.
GenomeReviews	Gene locus CCA_00773 in contig AE015925_GR.
KEGG	cca:CCA00773.
TIGR	CCA_00773.
Phylogenomic databases
HOGENOM	Q822B0.
OMA	QPIREKT.
Enzyme and pathway databases
BioCyc	CCAV227941:CCA_00773-MON.
BRENDA	1.1.1.158. 304519.
Family and domain databases
HAMAP	MF_00037. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00179. murB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_CHLCV

Accession

Primary (citable) accession number: Q822B0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)