Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q821S4 (MUDD_CHLCV) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional enzyme MurC/Ddl

Including the following 2 domains:

  1. UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
    Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase
  2. D-alanine--D-alanine ligase
    EC=6.3.2.4
    Alternative name(s):
    D-Ala-D-Ala ligase
    D-alanylalanine synthetase
Gene names
Name:murC/ddlA
Ordered Locus Names:CCA_00863
OrganismChlamydophila caviae (strain GPIC) [Complete proteome] [HAMAP]
Taxonomic identifier227941 [NCBI]
Taxonomic lineageBacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydophila ›

Protein attributes

Sequence length811 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP-Rule MF_00046

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00046

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00046

Subcellular location

Cytoplasm By similarity.

Sequence similarities

In the N-terminal section; belongs to the MurCDEF family.

In the C-terminal section; belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 811811Bifunctional enzyme MurC/Ddl HAMAP-Rule MF_00046
PRO_0000177913

Regions

Domain574 – 785212ATP-grasp
Nucleotide binding111 – 1177ATP Potential
Nucleotide binding607 – 66256ATP By similarity
Region1 – 450450UDP-N-acetylmuramate--alanine ligase HAMAP-Rule MF_00046
Region451 – 811361D-alanine--D-alanine ligase HAMAP-Rule MF_00046

Sites

Metal binding7391Magnesium or manganese 1 By similarity
Metal binding7521Magnesium or manganese 1 By similarity
Metal binding7521Magnesium or manganese 2 By similarity
Metal binding7541Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q821S4 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 096AC84D620947F2

FASTA81190,491
        10         20         30         40         50         60 
MNRKNHYHFI GIGGIGMSAL AHILLDRGYS VSGSDLNQGI TIDKLIAKGA AYLPGHKESY 

        70         80         90        100        110        120 
VPEEGTIIYG SGIAKDNVEY KEALRKQLPL VHRAELLALL MQEQTSILVS GSHGKTTVSS 

       130        140        150        160        170        180 
LITAIFQTAK KDPSYAIGGL NSQYLNGYSG SSEYFIAEAD ESDGSLKHYF PKVAVVTNLD 

       190        200        210        220        230        240 
NEHLSNFEGS KEKLAQTIEE FTRKVDDPNL CFYNGDCQEL KGRISGISYG FSQECALYIY 

       250        260        270        280        290        300 
SHRQEGWRSV FSLSFLGKDY LDIDLNLIGK HNVANAAAAI GVALTFGIDE ESIREALKSF 

       310        320        330        340        350        360 
SGVQRRMERK NTSEKFLFLE DYAHHPSEIS CTLRALRDAV GLRRIIAICQ PHRFSRLLYC 

       370        380        390        400        410        420 
LEEFFNAFQD ADEVILTDVY SAGEMPLDLP SPEKLAETIS LSSHVCCTYV PYDNVIEHLK 

       430        440        450        460        470        480 
QNIRVHDVCI SLGAGNIHTV GNALKDFEPK KLSVGVVCGG QSCEHDVSLL SARNVVQYLS 

       490        500        510        520        530        540 
PQHYDVQYFV INRQGLWSQV ANLDAGSDYN SKNYHVLSSK IAEALANLDF VLPILHGPFG 

       550        560        570        580        590        600 
EDGTLQGFLE IANKPYGGPS LLFSAISMDK IMTKRLAASV GVPVVPYQPL TLPTWKRTPE 

       610        620        630        640        650        660 
LCMRRILETF TFPMFVKTAH LGSSIGVFEV HNETELKAKI SEAFLYDTDV FIEESRLGSR 

       670        680        690        700        710        720 
EIEVSCLGDA CSCYYISEPH ERRGSKGFIG YEEKYGFNGK SSATIQYDLN LSEESKTRVK 

       730        740        750        760        770        780 
ELTERVYRVI QGKGSCRIDF FLDREGNFWL SEMNPIPGMT KSSPFLHDFA RLGWTFEQIV 

       790        800        810 
HQLIIAGLHK FDQKKKVSST FNKQCLLTAK S

« Hide

References

[1]"Genome sequence of Chlamydophila caviae (Chlamydia psittaci GPIC): examining the role of niche-specific genes in the evolution of the Chlamydiaceae."
Read T.D., Myers G.S.A., Brunham R.C., Nelson W.C., Paulsen I.T., Heidelberg J.F., Holtzapple E.K., Khouri H.M., Federova N.B., Carty H.A., Umayam L.A., Haft D.H., Peterson J.D., Beanan M.J., White O., Salzberg S.L., Hsia R.-C., McClarty G. expand/collapse author list , Rank R.G., Bavoil P.M., Fraser C.M.
Nucleic Acids Res. 31:2134-2147(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GPIC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE015925 Genomic DNA. Translation: AAP05604.1.
RefSeqNP_829726.1. NC_003361.3.

3D structure databases

ProteinModelPortalQ821S4.
ModBaseSearch...

Protein-protein interaction databases

STRING227941.CCA00863.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAP05604; AAP05604; CCA_00863.
GeneID1218548.
KEGGcca:CCA00863.
PATRIC20270857. VBIChlCav107360_0908.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
KOK01921.
K01924.
OMAREIEVSC.
ProtClustDBPRK14573.

Enzyme and pathway databases

BioCycCCAV227941:GH8L-870-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.1190.10. 1 hit.
3.40.50.20. 1 hit.
3.40.50.720. 1 hit.
3.90.190.20. 1 hit.
HAMAPMF_00046. MurC. Fused.
MF_00047. Dala_Dala_lig. Fused.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR016040. NAD(P)-bd_dom.
IPR016185. PreATP-grasp_dom.
IPR005758. UDP-N-AcMur_Ala_ligase.
[Graphical view]
PANTHERPTHR23135:SF5. PTHR23135:SF5. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
PF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMSSF53244. Mur_ligase_C. 1 hit.
SSF53623. Mur_ligase_cen. 1 hit.
SSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
TIGR01082. murC. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMUDD_CHLCV
AccessionPrimary (citable) accession number: Q821S4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: June 1, 2003
Last modified: May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents