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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Chlamydophila caviae (strain GPIC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathway:igluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei76 – 761SubstrateUniRule annotation
Metal bindingi225 – 2251ManganeseUniRule annotation
Metal bindingi245 – 2451Manganese; via tele nitrogenUniRule annotation
Binding sitei267 – 2671SubstrateUniRule annotation
Active sitei269 – 2691UniRule annotation
Metal bindingi292 – 2921ManganeseUniRule annotation
Binding sitei383 – 3831GTPUniRule annotation
Binding sitei414 – 4141GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi268 – 2736GTPUniRule annotation
Nucleotide bindingi508 – 5114GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCCAV227941:GH8L-954-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Synonyms:pckA
Ordered Locus Names:CCA_00916
OrganismiChlamydophila caviae (strain GPIC)
Taxonomic identifieri227941 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydophila
ProteomesiUP000002193 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 600600Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103596Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi227941.CCA00916.

Structurei

3D structure databases

ProteinModelPortaliQ821M4.
SMRiQ821M4. Positions 11-600.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni216 – 2183Substrate bindingUniRule annotation
Regioni381 – 3833Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q821M4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSAWSSEIQ HEGLKQWIQE VAELVTPDDI RICNGSDSEY AEVYGIMEKS
60 70 80 90 100
GVAIPLNPDV HPNCFLVRSS PEDVARVEQF TFICTTKKED AGPTNNWRDP
110 120 130 140 150
QEMRQELQGL FRGCMRGRTL YVIPFCMGPL NSPFSLIGVE LTDSPYVVCS
160 170 180 190 200
MKIMTRMGAE VLKSLGTTGS FHKCLHSVGA PLSPGEKDVA WPCNPKNMRI
210 220 230 240 250
VHFQDDSSVM SFGSGYGGNA LLGKKSVALR LASYIARSQG WLAEHMLIIG
260 270 280 290 300
VTNPEGQKKY FAASFPSACG KTNLAMLMPK IPGWEVECIG DDIAWIRPGP
310 320 330 340 350
DGRLYAVNPE FGFFGVAPGT SETTNPNALA TCKTNSVFTN VALTPDGNVW
360 370 380 390 400
WEGLTSQPPE GLIDWRGNPW QPGGAPAAHP NSRFTAPVKQ CPVLDPQWNS
410 420 430 440 450
PEGVPIEAII FGGRRSETIP LVYEALSWQH GVTIGASMSS ATTAAIVGEQ
460 470 480 490 500
GKLRHDPFAM LPFCGYNMAH YFDHWLSFAS NTSLKLPKIY GVNWFRKDKD
510 520 530 540 550
GNFIWPGFSD NLRVIEWIFR RTNGEESIAK KTPIGYLPEE SSLNLEGLNL
560 570 580 590 600
SSQALQDLLS VDVSGWLKEV ANVREYCKIF GSDLPQTITD ELFRIERELK
Length:600
Mass (Da):66,286
Last modified:June 1, 2003 - v1
Checksum:i5094CFC0E9691932
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015925 Genomic DNA. Translation: AAP05655.1.
RefSeqiWP_011006869.1. NC_003361.3.

Genome annotation databases

EnsemblBacteriaiAAP05655; AAP05655; CCA_00916.
KEGGicca:CCA00916.
PATRICi20270975. VBIChlCav107360_0961.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015925 Genomic DNA. Translation: AAP05655.1.
RefSeqiWP_011006869.1. NC_003361.3.

3D structure databases

ProteinModelPortaliQ821M4.
SMRiQ821M4. Positions 11-600.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi227941.CCA00916.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP05655; AAP05655; CCA_00916.
KEGGicca:CCA00916.
PATRICi20270975. VBIChlCav107360_0961.

Phylogenomic databases

eggNOGiCOG1274.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciCCAV227941:GH8L-954-MONOMER.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Chlamydophila caviae (Chlamydia psittaci GPIC): examining the role of niche-specific genes in the evolution of the Chlamydiaceae."
    Read T.D., Myers G.S.A., Brunham R.C., Nelson W.C., Paulsen I.T., Heidelberg J.F., Holtzapple E.K., Khouri H.M., Federova N.B., Carty H.A., Umayam L.A., Haft D.H., Peterson J.D., Beanan M.J., White O., Salzberg S.L., Hsia R.-C., McClarty G.
    , Rank R.G., Bavoil P.M., Fraser C.M.
    Nucleic Acids Res. 31:2134-2147(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GPIC.

Entry informationi

Entry nameiPCKG_CHLCV
AccessioniPrimary (citable) accession number: Q821M4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: June 1, 2003
Last modified: July 22, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.