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Protein

Folate synthesis bifunctional protein

Gene

folKP

Organism
Chlamydophila caviae (strain GPIC)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine = AMP + (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate.
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit. Magnesium is required for activity, even if it interacts primarily with the substrate.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi186 – 1861MagnesiumBy similarity
Binding sitei194 – 1941SubstrateBy similarity
Binding sitei266 – 2661SubstrateBy similarity
Binding sitei286 – 2861SubstrateBy similarity
Binding sitei356 – 3561SubstrateBy similarity
Binding sitei392 – 3921SubstrateBy similarity
Binding sitei426 – 4261SubstrateBy similarity
Binding sitei428 – 4281SubstrateBy similarity

GO - Molecular functioni

  1. 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. dihydropteroate synthase activity Source: UniProtKB-EC
  4. kinase activity Source: UniProtKB-KW
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. folic acid biosynthetic process Source: UniProtKB-KW
  2. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Folate biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCCAV227941:GH8L-1039-MONOMER.
UniPathwayiUPA00077; UER00155.
UPA00077; UER00156.

Names & Taxonomyi

Protein namesi
Recommended name:
Folate synthesis bifunctional protein
Including the following 2 domains:
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (EC:2.7.6.3)
Alternative name(s):
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
Short name:
PPPK
7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase
Short name:
HPPK
Dihydropteroate synthase (EC:2.5.1.15)
Short name:
DHPS
Alternative name(s):
Dihydropteroate pyrophosphorylase
Gene namesi
Name:folKP
Ordered Locus Names:CCA_00999
OrganismiChlamydophila caviae (strain GPIC)
Taxonomic identifieri227941 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydophila
ProteomesiUP000002193: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 447447Folate synthesis bifunctional proteinPRO_0000168238Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi227941.CCA00999.

Structurei

3D structure databases

ProteinModelPortaliQ821E2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini179 – 438260Pterin-bindingPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 165165HPPKAdd
BLAST
Regioni226 – 2272Substrate bindingBy similarity

Sequence similaritiesi

In the N-terminal section; belongs to the HPPK family.Curated
Contains 1 pterin-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0294.
KOiK13941.
OMAiFVNGACE.
OrthoDBiEOG67T5P5.

Family and domain databases

Gene3Di3.20.20.20. 1 hit.
3.30.70.560. 1 hit.
InterProiIPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000550. Hppk.
IPR000489. Pterin-binding.
[Graphical view]
PfamiPF01288. HPPK. 1 hit.
PF00809. Pterin_bind. 1 hit.
[Graphical view]
SUPFAMiSSF51717. SSF51717. 1 hit.
SSF55083. SSF55083. 1 hit.
TIGRFAMsiTIGR01496. DHPS. 1 hit.
TIGR01498. folK. 1 hit.
PROSITEiPS00792. DHPS_1. 1 hit.
PS00793. DHPS_2. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q821E2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTAQFICLS LGSNLGNRFE IFRKAFALLK ELEIEDLQSS IILETKALLL
60 70 80 90 100
PGSPKEWDLP FFNSVLIGKT TLSPKQLLSG VKQIERRLGR DVNALPWSPR
110 120 130 140 150
VLDIDILLYG DENHQQEDVK IPHERITERP FLLSLIASLC PTRIFHKPGS
160 170 180 190 200
EYHLKSFGEI AHLLPCPQEM ILNSFSPNTL LMGVVNVTDN SISDEGLYLE
210 220 230 240 250
ASKAVAHAEK LFAQGASVID FGGQATNPKV QQLLNVEQEW SRLEPVLKLL
260 270 280 290 300
AEKWAGRRQY PDISLDTFYP EIIKRALEIY PIRWINDVSG GSKEMAEIAR
310 320 330 340 350
DANLLLVINH SCSLPPRPDK TLAFTTCASD QLLSWGEKQI EAFVALGLRQ
360 370 380 390 400
DQIIFDPGIG FGTTQIQALN VLHKMKKFRK LGCATLVGHS RKSCFSLLGK
410 420 430 440
YDAKDRDWET VSLSVLLQQQ GVNYLRVHDV EANQRVLSAA AWSGVHV
Length:447
Mass (Da):50,077
Last modified:June 1, 2003 - v1
Checksum:iDA22B1F8B954599D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015925 Genomic DNA. Translation: AAP05738.1.
RefSeqiNP_829860.1. NC_003361.3.

Genome annotation databases

EnsemblBacteriaiAAP05738; AAP05738; CCA_00999.
GeneIDi1218687.
KEGGicca:CCA00999.
PATRICi20271153. VBIChlCav107360_1048.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015925 Genomic DNA. Translation: AAP05738.1.
RefSeqiNP_829860.1. NC_003361.3.

3D structure databases

ProteinModelPortaliQ821E2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi227941.CCA00999.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP05738; AAP05738; CCA_00999.
GeneIDi1218687.
KEGGicca:CCA00999.
PATRICi20271153. VBIChlCav107360_1048.

Phylogenomic databases

eggNOGiCOG0294.
KOiK13941.
OMAiFVNGACE.
OrthoDBiEOG67T5P5.

Enzyme and pathway databases

UniPathwayiUPA00077; UER00155.
UPA00077; UER00156.
BioCyciCCAV227941:GH8L-1039-MONOMER.

Family and domain databases

Gene3Di3.20.20.20. 1 hit.
3.30.70.560. 1 hit.
InterProiIPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000550. Hppk.
IPR000489. Pterin-binding.
[Graphical view]
PfamiPF01288. HPPK. 1 hit.
PF00809. Pterin_bind. 1 hit.
[Graphical view]
SUPFAMiSSF51717. SSF51717. 1 hit.
SSF55083. SSF55083. 1 hit.
TIGRFAMsiTIGR01496. DHPS. 1 hit.
TIGR01498. folK. 1 hit.
PROSITEiPS00792. DHPS_1. 1 hit.
PS00793. DHPS_2. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Chlamydophila caviae (Chlamydia psittaci GPIC): examining the role of niche-specific genes in the evolution of the Chlamydiaceae."
    Read T.D., Myers G.S.A., Brunham R.C., Nelson W.C., Paulsen I.T., Heidelberg J.F., Holtzapple E.K., Khouri H.M., Federova N.B., Carty H.A., Umayam L.A., Haft D.H., Peterson J.D., Beanan M.J., White O., Salzberg S.L., Hsia R.-C., McClarty G.
    , Rank R.G., Bavoil P.M., Fraser C.M.
    Nucleic Acids Res. 31:2134-2147(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GPIC.

Entry informationi

Entry nameiFOLKP_CHLCV
AccessioniPrimary (citable) accession number: Q821E2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: June 1, 2003
Last modified: February 4, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.