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Protein

Folate synthesis bifunctional protein

Gene

folKP

Organism
Chlamydophila caviae (strain GPIC)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 6-hydroxymethyl-7,8-dihydropterin = AMP + 6-hydroxymethyl-7,8-dihydropterin diphosphate.
6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.

Cofactori

Mg2+By similarity

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. 7,8-dihydroneopterin aldolase (folB)
  4. Folate synthesis bifunctional protein (folKP)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Folate synthesis bifunctional protein (folKP)
  2. no protein annotated in this organism
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi186MagnesiumBy similarity1
Binding sitei2266-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei2666-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei2866-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei3566-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei3926-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Folate biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00077; UER00155.
UPA00077; UER00156.

Names & Taxonomyi

Protein namesi
Recommended name:
Folate synthesis bifunctional protein
Including the following 2 domains:
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase (EC:2.7.6.3)
Short name:
HPPK
Alternative name(s):
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase
7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase
Short name:
PPPK
Dihydropteroate synthase (EC:2.5.1.15)
Short name:
DHPS
Alternative name(s):
Dihydropteroate pyrophosphorylase
Gene namesi
Name:folKP
Ordered Locus Names:CCA_00999
OrganismiChlamydophila caviae (strain GPIC)
Taxonomic identifieri227941 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
Proteomesi
  • UP000002193 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001682381 – 447Folate synthesis bifunctional proteinAdd BLAST447

Interactioni

Protein-protein interaction databases

STRINGi227941.CCA00999.

Structurei

3D structure databases

ProteinModelPortaliQ821E2.
SMRiQ821E2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini179 – 438Pterin-bindingPROSITE-ProRule annotationAdd BLAST260

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 165HPPKAdd BLAST165
Regioni181 – 447DHPSAdd BLAST267
Regioni426 – 4286-hydroxymethyl-7,8-dihydropterin diphosphate bindingBy similarity3

Sequence similaritiesi

In the C-terminal section; belongs to the DHPS family.Curated
In the N-terminal section; belongs to the HPPK family.Curated
Contains 1 pterin-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105EEI. Bacteria.
COG0294. LUCA.
COG0801. LUCA.
KOiK13941.
OMAiKTHVMAI.
OrthoDBiPOG091H00BJ.

Family and domain databases

CDDicd00739. DHPS. 1 hit.
cd00483. HPPK. 1 hit.
Gene3Di3.20.20.20. 1 hit.
3.30.70.560. 1 hit.
InterProiIPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000550. Hppk.
IPR000489. Pterin-binding_dom.
[Graphical view]
PfamiPF01288. HPPK. 1 hit.
PF00809. Pterin_bind. 1 hit.
[Graphical view]
SUPFAMiSSF51717. SSF51717. 1 hit.
SSF55083. SSF55083. 1 hit.
TIGRFAMsiTIGR01496. DHPS. 1 hit.
TIGR01498. folK. 1 hit.
PROSITEiPS00792. DHPS_1. 1 hit.
PS00793. DHPS_2. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q821E2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTAQFICLS LGSNLGNRFE IFRKAFALLK ELEIEDLQSS IILETKALLL
60 70 80 90 100
PGSPKEWDLP FFNSVLIGKT TLSPKQLLSG VKQIERRLGR DVNALPWSPR
110 120 130 140 150
VLDIDILLYG DENHQQEDVK IPHERITERP FLLSLIASLC PTRIFHKPGS
160 170 180 190 200
EYHLKSFGEI AHLLPCPQEM ILNSFSPNTL LMGVVNVTDN SISDEGLYLE
210 220 230 240 250
ASKAVAHAEK LFAQGASVID FGGQATNPKV QQLLNVEQEW SRLEPVLKLL
260 270 280 290 300
AEKWAGRRQY PDISLDTFYP EIIKRALEIY PIRWINDVSG GSKEMAEIAR
310 320 330 340 350
DANLLLVINH SCSLPPRPDK TLAFTTCASD QLLSWGEKQI EAFVALGLRQ
360 370 380 390 400
DQIIFDPGIG FGTTQIQALN VLHKMKKFRK LGCATLVGHS RKSCFSLLGK
410 420 430 440
YDAKDRDWET VSLSVLLQQQ GVNYLRVHDV EANQRVLSAA AWSGVHV
Length:447
Mass (Da):50,077
Last modified:June 1, 2003 - v1
Checksum:iDA22B1F8B954599D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015925 Genomic DNA. Translation: AAP05738.1.
RefSeqiWP_011006951.1. NC_003361.3.

Genome annotation databases

EnsemblBacteriaiAAP05738; AAP05738; CCA_00999.
KEGGicca:CCA_00999.
PATRICi20271153. VBIChlCav107360_1048.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015925 Genomic DNA. Translation: AAP05738.1.
RefSeqiWP_011006951.1. NC_003361.3.

3D structure databases

ProteinModelPortaliQ821E2.
SMRiQ821E2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi227941.CCA00999.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP05738; AAP05738; CCA_00999.
KEGGicca:CCA_00999.
PATRICi20271153. VBIChlCav107360_1048.

Phylogenomic databases

eggNOGiENOG4105EEI. Bacteria.
COG0294. LUCA.
COG0801. LUCA.
KOiK13941.
OMAiKTHVMAI.
OrthoDBiPOG091H00BJ.

Enzyme and pathway databases

UniPathwayiUPA00077; UER00155.
UPA00077; UER00156.

Family and domain databases

CDDicd00739. DHPS. 1 hit.
cd00483. HPPK. 1 hit.
Gene3Di3.20.20.20. 1 hit.
3.30.70.560. 1 hit.
InterProiIPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000550. Hppk.
IPR000489. Pterin-binding_dom.
[Graphical view]
PfamiPF01288. HPPK. 1 hit.
PF00809. Pterin_bind. 1 hit.
[Graphical view]
SUPFAMiSSF51717. SSF51717. 1 hit.
SSF55083. SSF55083. 1 hit.
TIGRFAMsiTIGR01496. DHPS. 1 hit.
TIGR01498. folK. 1 hit.
PROSITEiPS00792. DHPS_1. 1 hit.
PS00793. DHPS_2. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFOLKP_CHLCV
AccessioniPrimary (citable) accession number: Q821E2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: June 1, 2003
Last modified: November 30, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.