Reviewed, UniProtKB/Swiss-Prot Q821A5 (RLMG_SHIFL)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase G
    EC=2.1.1.52
Alternative name(s):
    rRNA (guanine-N(2)-)-methyltransferase rlmG
    23S rRNA m2G1835 methyltransferase

Gene names

Name:	rlmG
Ordered Locus Names:	SF3124, S3331

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(2)-methylguanine. HAMAP MF_01859
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA methylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366524

Sequences

	Sequence		Length	Mass (Da)
	Q821A5-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 01FB17B8FA75E903	FASTA	378	42,267
	10 20 30 40 50 60 MSHLDNGFRS LTLQRFPATD DVNPLQAWEA ADEYLLQQLD DTEILGPVLI LNDAFGALSC 70 80 90 100 110 120 ALAEHKPYSI GDSYISELAT RENLRLNGID ESSVKFLDST ADYPQQPGVV LIKVPKTLAL 130 140 150 160 170 180 LEQQLRALRK VVTSDTRIIA GAKARDIHTS TLELFEKVLG PTTTTLAWKK ARLINCTFNE 190 200 210 220 230 240 PPLADAPQTV SWKLEGTDWT IHNHANVFSR TGLDIGARFF MQHLPENLEG EIVDLGCGNG 250 260 270 280 290 300 VIGLTLLDKN PQAKVVFVDE SPMAVASSRL NVETNMPEAL DRSEFMINNA LSGVEPFRFN 310 320 330 340 350 360 AVLCNPPFHQ QHALTDNVAW EMFHYARRCL KINGELYIVA NRHLDYFHKL KKIFGNCTTI 370 ATNNKFVVLK AVKLGRRR

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005674 Genomic DNA. Translation: AAN44596.1. AE014073 Genomic DNA. Translation: AAP18409.1.
RefSeq	NP_708889.1. NP_838598.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1026843. 1078756.
GenomeReviews	Gene locus SF3124 in contig AE005674_GR. Gene locus S3331 in contig AE014073_GR.
KEGG	sfl:SF3124. sfx:S3331.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q821A5.
OMA	NRHLGYH.
Enzyme and pathway databases
BioCyc	SFLE198214:AAN44596.1-MON.
Family and domain databases
HAMAP	MF_01859. [Tree]
InterPro	IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_SHIFL

Accession

Primary (citable) accession number: Q821A5
Secondary accession number(s): Q7BZU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)