Q820Z0 (TRMD_TROW8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	TW307

Organism

Tropheryma whipplei (strain TW08/27) (Whipple's bacillus) [Complete proteome] [HAMAP]

Taxonomic identifier

218496 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Tropheryma

Protein attributes

Sequence length	234 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 234	234	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000060489
Regions
	Region	134 – 139	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	110	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q820Z0 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 69BD8D4C945D03F9	FASTA	234	26,461
	10 20 30 40 50 60 MFVMRCDFVS IFPEYFDVLD ISLIGKARRN GLLDLRVHNL REYSEAGRVD SSPYGGGPGM 70 80 90 100 110 120 VMSAEPWARA IEHIATGESL VVFPSPSGQP YSHDLAQSLS SEMHIVFCCG RYEGIDNRIY 130 140 150 160 170 180 EWTATRLRSS GISIGDYVLN GGEIAALVIL EGFVRFIPGV LGNPESLVEE SYQYNLLEYP 190 200 210 220 230 VYTKPAVWRG LEVPDILLSG NHDLIREWRY KKQLEITQKT RPDLYSTHIY ETDS

References

[1]

"Sequencing and analysis of the genome of the Whipple's disease bacterium Tropheryma whipplei." Bentley S.D., Maiwald M., Murphy L.D., Pallen M.J., Yeats C.A., Dover L.G., Norbertczak H.T., Besra G.S., Quail M.A., Harris D.E., von Herbay A., Goble A., Rutter S., Squares R., Squares S., Barrell B.G., Parkhill J., Relman D.A. Lancet 361:637-644(2003) [PubMed: 12606174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW08/27.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX251411 Genomic DNA. Translation: CAD66981.1.
RefSeq	NP_789243.1. NC_004551.1.
3D structure databases
ProteinModelPortal	Q820Z0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q820Z0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1064596.
GenomeReviews	Gene locus TW307 in contig BX072543_GR.
KEGG	tws:TW307.
PATRIC	23999071. VBITroWhi42739_0371.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0336.
HOGENOM	HBG285805.
OMA	VCGRFEG.
PhylomeDB	Q820Z0.
ProtClustDB	PRK00026.
Enzyme and pathway databases
BioCyc	TWHI218496:TW0288-MONOMER.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. TrmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_TROW8

Accession

Primary (citable) accession number: Q820Z0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program