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Protein

tRNA-specific 2-thiouridylase MnmA

Gene

mnmA

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.UniRule annotation

Catalytic activityi

A [protein]-S-sulfanyl-L-cysteine + uridine(34) in tRNA + ATP + reduced acceptor = a [protein]-L-cysteine + 2-thiouridine(34) in tRNA + AMP + diphosphate + acceptor.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Active sitei104NucleophileUniRule annotation1
Binding sitei128ATP; via amide nitrogenUniRule annotation1
Active sitei200Cysteine persulfide intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 20ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-specific 2-thiouridylase MnmAUniRule annotation (EC:2.8.1.13UniRule annotation)
Gene namesi
Name:mnmAUniRule annotation
Synonyms:trmU
Ordered Locus Names:CBU_1147
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
Proteomesi
  • UP000002671 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002445331 – 362tRNA-specific 2-thiouridylase MnmAAdd BLAST362

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi104 ↔ 200AlternateUniRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ820W2.

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei129Interaction with tRNAUniRule annotation1
Sitei345Interaction with tRNAUniRule annotation1

Protein-protein interaction databases

STRINGi227377.CBU_1147.

Structurei

3D structure databases

ProteinModelPortaliQ820W2.
SMRiQ820W2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni99 – 101Interaction with target base in tRNAUniRule annotation3
Regioni150 – 152Interaction with tRNAUniRule annotation3

Sequence similaritiesi

Belongs to the MnmA/TRMU family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCJ. Bacteria.
COG0482. LUCA.
HOGENOMiHOG000218046.
KOiK00566.
OMAiAVCTGHY.

Family and domain databases

CDDicd01998. tRNA_Me_trans. 1 hit.
Gene3Di2.30.30.280. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00144. tRNA_thiouridyl_MnmA. 1 hit.
InterProiView protein in InterPro
IPR023382. Adenine_a_hdrlase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004506. tRNA-specific_2-thiouridylase.
PANTHERiPTHR11933. PTHR11933. 1 hit.
TIGRFAMsiTIGR00420. trmU. 1 hit.

Sequencei

Sequence statusi: Complete.

Q820W2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPNFEQNQVI AVGLSGGVDS SVAALVLKEK GYEVIGLFMQ NWETDSKDPF
60 70 80 90 100
CTAEQDLSDA KAIADHIGIP LYVVNFSKAY WNHVFQHCLD EFAQGRTPNP
110 120 130 140 150
DVWCNREIKF KSLLDHAKKL GATHLATGHY ACIQNENNEY RLLKSNDSHK
160 170 180 190 200
DQSYFLHLLN QYQLANSVFP IGGYQKSEVR AIAKKRGFIN HAKKDSTGIC
210 220 230 240 250
FIGERKFKDF LNEFLLAQPG NIETSEGKII GKHDGIMFYT VGQRKGLHIG
260 270 280 290 300
GRPDAGEAPW YVVDKDVKRN VLIVVQGYEH PLLYSQELTC TNLHWIRDTE
310 320 330 340 350
PSFPLTCKAK TRCRQADQTC VVTRLDNDHC HVQFEHPQRA ITRGQSVVFY
360
LGNECLGGGI IN
Length:362
Mass (Da):40,860
Last modified:June 1, 2003 - v1
Checksum:i7834C2267F2F402C
GO

Sequence cautioni

The sequence AAO90659 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA. Translation: AAO90659.2. Different initiation.
RefSeqiNP_820145.2. NC_002971.3.
WP_032138352.1. NZ_CCYB01000037.1.

Genome annotation databases

EnsemblBacteriaiAAO90659; AAO90659; CBU_1147.
GeneIDi1209049.
KEGGicbu:CBU_1147.
PATRICifig|227377.7.peg.1140.

Similar proteinsi

Entry informationi

Entry nameiMNMA_COXBU
AccessioniPrimary (citable) accession number: Q820W2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 1, 2003
Last modified: November 22, 2017
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families