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Q820V5

- FABI_ENTFA

UniProt

Q820V5 - FABI_ENTFA

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Protein
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI
Gene
fabI, EF_0282
Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism By similarity.

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Enzyme regulationi

Inhibited by triclosan and its diphenyl ether analgues.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121NAD; via carbonyl oxygen By similarity
Binding sitei39 – 391NAD By similarity
Binding sitei89 – 891NAD; via carbonyl oxygen By similarity
Binding sitei92 – 921Substrate; via amide nitrogen and carbonyl oxygen By similarity
Active sitei142 – 1421Proton acceptor By similarity
Active sitei152 – 1521Proton acceptor By similarity
Binding sitei159 – 1591NAD By similarity
Sitei197 – 1971Involved in acyl-ACP binding By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 192NAD By similarity
Nucleotide bindingi61 – 622NAD By similarity
Nucleotide bindingi188 – 1925NAD By similarity

GO - Molecular functioni

  1. enoyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. fatty acid elongation Source: UniProtKB
  2. protein homotetramerization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciEFAE226185:GHI1-289-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
Short name:
ENR
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene namesi
Name:fabI
Ordered Locus Names:EF_0282
OrganismiEnterococcus faecalis (strain ATCC 700802 / V583)
Taxonomic identifieri226185 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
ProteomesiUP000001415: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 250250Enoyl-[acyl-carrier-protein] reductase [NADH] FabI
PRO_0000407977Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.

Protein-protein interaction databases

STRINGi226185.EF0282.

Structurei

3D structure databases

ProteinModelPortaliQ820V5.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0623.
KOiK00208.
OMAiCFATIKE.
OrthoDBiEOG6HF644.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.

Sequencei

Sequence statusi: Complete.

Q820V5-1 [UniParc]FASTAAdd to Basket

« Hide

MFLQNKNVVV MGVANKKSIA WGCAKALKDQ GANVIYTYQN ERMKKQVVKL    50
ADENDLLVEC DVASDASIQA AFETIKNEVG TIDGLVHAIA FAKKEELSGN 100
VSDITRDGFL LAQDISSYSL LAVTHYAKPL LNPGSGIVTL TYLGSERAIP 150
NYNMMGIAKA SLETAVKYLA FELAADKIRV NGISAGAIKT LAVTGVKDYD 200
QLISISNERT PDKTGVTIEE VGNTCAFLVS DLASGVVGDI IYVDKGVHLT 250
Length:250
Mass (Da):26,767
Last modified:June 1, 2003 - v1
Checksum:iA0A4DA491D22F78E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016830 Genomic DNA. Translation: AAO80145.1.
RefSeqiNP_814074.1. NC_004668.1.

Genome annotation databases

EnsemblBacteriaiAAO80145; AAO80145; EF_0282.
GeneIDi1199200.
KEGGiefa:EF0282.
PATRICi21851013. VBIEntFae7065_0260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016830 Genomic DNA. Translation: AAO80145.1 .
RefSeqi NP_814074.1. NC_004668.1.

3D structure databases

ProteinModelPortali Q820V5.
ModBasei Search...

Protein-protein interaction databases

STRINGi 226185.EF0282.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO80145 ; AAO80145 ; EF_0282 .
GeneIDi 1199200.
KEGGi efa:EF0282.
PATRICi 21851013. VBIEntFae7065_0260.

Phylogenomic databases

eggNOGi COG0623.
KOi K00208.
OMAi CFATIKE.
OrthoDBi EOG6HF644.

Enzyme and pathway databases

UniPathwayi UPA00094 .
BioCyci EFAE226185:GHI1-289-MONOMER.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR014358. Enoyl-ACP_Rdtase_NADH.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PIRSFi PIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSi PR00081. GDHRDH.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700802 / V583.
  2. "Mechanism and inhibition of saFabI, the enoyl reductase from Staphylococcus aureus."
    Xu H., Sullivan T.J., Sekiguchi J., Kirikae T., Ojima I., Stratton C.F., Mao W., Rock F.L., Alley M.R., Johnson F., Walker S.G., Tonge P.J.
    Biochemistry 47:4228-4236(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.

Entry informationi

Entry nameiFABI_ENTFA
AccessioniPrimary (citable) accession number: Q820V5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: June 1, 2003
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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