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Q820M6

- SYI_NITEU

UniProt

Q820M6 - SYI_NITEU

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Nitrosomonas europaea (strain ATCC 19718 / NBRC 14298)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei570 – 5701Aminoacyl-adenylateUniRule annotation
Binding sitei614 – 6141ATPUniRule annotation
Metal bindingi902 – 9021ZincUniRule annotation
Metal bindingi905 – 9051ZincUniRule annotation
Metal bindingi922 – 9221ZincUniRule annotation
Metal bindingi925 – 9251ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciNEUR228410:GJNO-1172-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:NE1149
OrganismiNitrosomonas europaea (strain ATCC 19718 / NBRC 14298)
Taxonomic identifieri228410 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas
ProteomesiUP000001416: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 939939Isoleucine--tRNA ligasePRO_0000098431Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi228410.NE1149.

Structurei

3D structure databases

ProteinModelPortaliQ820M6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionAdd
BLAST
Motifi611 – 6155"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.
PhylomeDBiQ820M6.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q820M6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTIDYKKTLN LLDTSFPMRG DLARREPAML KAWQERNLYR KIRAISQGRP
60 70 80 90 100
KFILHDGPPY ANGDIHIGHA VNKILKDIII KSKTLSGFDA PYVPGWDCHG
110 120 130 140 150
LPIEHQIEKK YGKHLPADQV RKLCRAFAQE QIDRQKADFM RLGVLGDWEH
160 170 180 190 200
PYLTMNYAIE AGIIRALGKI FRNGHLYQGQ KPVNWCIDCG SALAEAEVEY
210 220 230 240 250
ENKRSPAIDV GFEIIGRKDV CHPLAGVMAA EIPAGTRIFA VIWTTTPWTL
260 270 280 290 300
PANQAVCVHP EFDYSLVSTS RGWLLLASEL TNACLARYQL EGRVVATCKG
310 320 330 340 350
LALEGLSLQH PFADRIVKVI CGRHVTLEAG TGLVHTAPAH GLDDYFIGQQ
360 370 380 390 400
YGLPSDSPVK GDGKFSEQIS LVGGMFVWKA NDVVIDTLRS SGHLLHAEEI
410 420 430 440 450
EHSYPHCWRH KTPIIFRATP QWFIGMQRQT AESQSFGESL RDLALRAVEL
460 470 480 490 500
TRFYPAWGRA RLEAMIGNRP DWCISRQRNW GVPMTFFIHK EAHTLHPRTP
510 520 530 540 550
ELLEKVAGLV EQQGIEAWFS LDATELLGEE AQHYQKLTDT LDVWFDSGTT
560 570 580 590 600
HETVLKQNIQ LRHPADLYLE GSDQHRGWFQ SSLLTGCAID GCAPYTALLT
610 620 630 640 650
HGFVVDGQGY KMSKSKGNVI APQKIADTLG ADILRLWVAS TDYSGELSIS
660 670 680 690 700
DEILKRTVET YRRIRNTLRF LLANLADFNL AADALPPAEW VEIDRYMLAY
710 720 730 740 750
TAALQNDLLG FYERYEFHQA VARLHHFCSE DLGGFYLDIL KDRLYTSMAN
760 770 780 790 800
GIPRRSAQNA LYHIVHSLVR LFAPVLSFTA EEVWQELGES AEDSVFLHTW
810 820 830 840 850
HCFPDQSEIL SDAQILIPRW QRLRELRARV LKQLEDARIQ GEIGSSLAAI
860 870 880 890 900
VEIHAAGEDF ALLDSLGDDL RFVLITSEVH LQRVDDAAGE VIRVTASPHM
910 920 930
KCERCWHYRQ DVGSVPEHSS LCSRCVSNLA GSGEYRRFA
Length:939
Mass (Da):105,892
Last modified:June 1, 2003 - v1
Checksum:i6215FB6B12C10232
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL954747 Genomic DNA. Translation: CAD85060.1.
RefSeqiNP_841206.1. NC_004757.1.

Genome annotation databases

EnsemblBacteriaiCAD85060; CAD85060; NE1149.
GeneIDi1082091.
KEGGineu:NE1149.
PATRICi22713342. VBINitEur56163_1272.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL954747 Genomic DNA. Translation: CAD85060.1 .
RefSeqi NP_841206.1. NC_004757.1.

3D structure databases

ProteinModelPortali Q820M6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 228410.NE1149.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD85060 ; CAD85060 ; NE1149 .
GeneIDi 1082091.
KEGGi neu:NE1149.
PATRICi 22713342. VBINitEur56163_1272.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.
PhylomeDBi Q820M6.

Enzyme and pathway databases

BioCyci NEUR228410:GJNO-1172-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea."
    Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L., Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A., Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J.
    J. Bacteriol. 185:2759-2773(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 19718 / NBRC 14298.

Entry informationi

Entry nameiSYI_NITEU
AccessioniPrimary (citable) accession number: Q820M6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2003
Last modified: October 29, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3