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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Nitrosomonas europaea (strain ATCC 19718 / NBRC 14298)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei570 – 5701Aminoacyl-adenylateUniRule annotation
Binding sitei614 – 6141ATPUniRule annotation
Metal bindingi902 – 9021ZincUniRule annotation
Metal bindingi905 – 9051ZincUniRule annotation
Metal bindingi922 – 9221ZincUniRule annotation
Metal bindingi925 – 9251ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciNEUR228410:GJNO-1172-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:NE1149
OrganismiNitrosomonas europaea (strain ATCC 19718 / NBRC 14298)
Taxonomic identifieri228410 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas
ProteomesiUP000001416 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 939939Isoleucine--tRNA ligasePRO_0000098431Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi228410.NE1149.

Structurei

3D structure databases

ProteinModelPortaliQ820M6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionAdd
BLAST
Motifi611 – 6155"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.
PhylomeDBiQ820M6.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q820M6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIDYKKTLN LLDTSFPMRG DLARREPAML KAWQERNLYR KIRAISQGRP
60 70 80 90 100
KFILHDGPPY ANGDIHIGHA VNKILKDIII KSKTLSGFDA PYVPGWDCHG
110 120 130 140 150
LPIEHQIEKK YGKHLPADQV RKLCRAFAQE QIDRQKADFM RLGVLGDWEH
160 170 180 190 200
PYLTMNYAIE AGIIRALGKI FRNGHLYQGQ KPVNWCIDCG SALAEAEVEY
210 220 230 240 250
ENKRSPAIDV GFEIIGRKDV CHPLAGVMAA EIPAGTRIFA VIWTTTPWTL
260 270 280 290 300
PANQAVCVHP EFDYSLVSTS RGWLLLASEL TNACLARYQL EGRVVATCKG
310 320 330 340 350
LALEGLSLQH PFADRIVKVI CGRHVTLEAG TGLVHTAPAH GLDDYFIGQQ
360 370 380 390 400
YGLPSDSPVK GDGKFSEQIS LVGGMFVWKA NDVVIDTLRS SGHLLHAEEI
410 420 430 440 450
EHSYPHCWRH KTPIIFRATP QWFIGMQRQT AESQSFGESL RDLALRAVEL
460 470 480 490 500
TRFYPAWGRA RLEAMIGNRP DWCISRQRNW GVPMTFFIHK EAHTLHPRTP
510 520 530 540 550
ELLEKVAGLV EQQGIEAWFS LDATELLGEE AQHYQKLTDT LDVWFDSGTT
560 570 580 590 600
HETVLKQNIQ LRHPADLYLE GSDQHRGWFQ SSLLTGCAID GCAPYTALLT
610 620 630 640 650
HGFVVDGQGY KMSKSKGNVI APQKIADTLG ADILRLWVAS TDYSGELSIS
660 670 680 690 700
DEILKRTVET YRRIRNTLRF LLANLADFNL AADALPPAEW VEIDRYMLAY
710 720 730 740 750
TAALQNDLLG FYERYEFHQA VARLHHFCSE DLGGFYLDIL KDRLYTSMAN
760 770 780 790 800
GIPRRSAQNA LYHIVHSLVR LFAPVLSFTA EEVWQELGES AEDSVFLHTW
810 820 830 840 850
HCFPDQSEIL SDAQILIPRW QRLRELRARV LKQLEDARIQ GEIGSSLAAI
860 870 880 890 900
VEIHAAGEDF ALLDSLGDDL RFVLITSEVH LQRVDDAAGE VIRVTASPHM
910 920 930
KCERCWHYRQ DVGSVPEHSS LCSRCVSNLA GSGEYRRFA
Length:939
Mass (Da):105,892
Last modified:June 1, 2003 - v1
Checksum:i6215FB6B12C10232
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL954747 Genomic DNA. Translation: CAD85060.1.
RefSeqiNP_841206.1. NC_004757.1.
WP_011111740.1. NC_004757.1.

Genome annotation databases

EnsemblBacteriaiCAD85060; CAD85060; NE1149.
KEGGineu:NE1149.
PATRICi22713342. VBINitEur56163_1272.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL954747 Genomic DNA. Translation: CAD85060.1.
RefSeqiNP_841206.1. NC_004757.1.
WP_011111740.1. NC_004757.1.

3D structure databases

ProteinModelPortaliQ820M6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi228410.NE1149.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD85060; CAD85060; NE1149.
KEGGineu:NE1149.
PATRICi22713342. VBINitEur56163_1272.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.
PhylomeDBiQ820M6.

Enzyme and pathway databases

BioCyciNEUR228410:GJNO-1172-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the ammonia-oxidizing bacterium and obligate chemolithoautotroph Nitrosomonas europaea."
    Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L., Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A., Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J.
    J. Bacteriol. 185:2759-2773(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 19718 / NBRC 14298.

Entry informationi

Entry nameiSYI_NITEU
AccessioniPrimary (citable) accession number: Q820M6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2003
Last modified: May 27, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.